No effect of 50 Hz magnetic field observed in a pilot study on pentylenetetrazol-induced seizures and mortality in mice

Keskil, I. S.; Keskil, Zuhal Aktuna; Canseven, Ayşe G.; Seyhan, Nesrin

doi:10.1016/s0920-1211(00)00193-5

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…Although Klauenberg et al (27) and Ossenkopp et al (28) showed the shortening effect of MF on the seizure duration, Keskil et al (29) found no relation between them. In another study (30), using a similar method to that in our present study, it was shown that the seizure latency decreased but the seizure duration increased.…”

Section: Discussionmentioning

confidence: 96%

Investigation of the effects of magnetic field exposure on febrile seizure latency, seizure duration, and electroencephalographic recordings in a rat febrile convulsion model

Demi̇r¹,

Gültürk²,

Çançalar³

et al. 2014

Turk J Med Sci

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This study covers the design, synthesis, characterization, and cytotoxic activity of a series of uracil C-Mannich bases. Among them, one hybrid compound (1), a molecular combination of the potential antimetabolite substituted uracil and nitrogen mustard, having potential alkylating capability, was prepared as a Mannich base. The other compound was synthesized with the replacement of chlorines in the ethyl chains with hydroxyl groups for testing for anticancer activity. Some of Mannich bases having several amino groups with different pKa values were also synthesized and investigated in terms of cytotoxic activity. Their chemical structures were confirmed by means of their UV, IR, 1 H-NMR, 13 C-NMR, and MS data. Compounds 6 with diethylamine and 8 with piperazine are reported for the first time in the literature and compounds 1, 4, and 5 containing nitrogen mustard, pyrolidine, and diethanolamine, respectively, as amine function are reported for first time with detailed spectral data herein. Morpholine, piperidine, and dimethylamine were used in Mannich reactions for the synthesis of compounds 2, 3 and 7. We assessed their biological activities using MTT assays on 3 human cell lines: HeLa (cervix adenocarcinoma), MCF7 (breast adenocarcinoma), and A431 (skin epidermoid carcinoma). While compounds 2-8 have the potential to deaminate, forming ortho-quinone methides, which would be capable of alkylating cellular thiols, compound 1 has the potential to give aziridinium ion for nucleophilic alkylation. Our results are discussed in terms of the significance of these compounds in pharmaceutical use.

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Section: Discussionmentioning

confidence: 96%

Investigation of the effects of magnetic field exposure on febrile seizure latency, seizure duration, and electroencephalographic recordings in a rat febrile convulsion model

Demi̇r¹,

Gültürk²,

Çançalar³

et al. 2014

Turk J Med Sci

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“…at a dose of 60 mg/kg in 0.1 ml (Ossenkopp & Cain 1991, Lö sher & Fiedler 1996. This is the submaximal dose, between the 50% effective dose (ED 50 ) of 33 mg/kg and the median effective lethal dose (LD 50 ) of 75 mg/kg (Keskil et al 2001).…”

Section: Exposure Proceduresmentioning

confidence: 99%

“…Since MF has been discussed as having a therapeutic potential (Anninos et al 1991), we recently investigated the impact of 50 Hz, 0.2 mT MF exposure in mice and showed that it had no significant effect on the PTZ-induced seizure latency, total seizure duration or mortality compared with controls (Keskil et al 2001). In the present study, we investigated whether MF exposure has different effects when applied before versus after PTZ injection.…”

Section: Introductionmentioning

confidence: 99%

Pentylenetetrazol-induced seizures are not altered by pre- or post-drug exposure to a 50 Hz magnetic field

Canseven

Keskil

et al. 2007

International Journal of Radiation Biology

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“…In experimental mice, myoclonic seizure (MCL), including twitches and straub tail reaction, clonic seizure (CL), tonic flexion (TF), and tonic extension (TE), were observed after PTZ administration (3 -5). Among these behaviors, MCL and CL observed in mice are mainly used as a model of human absence seizure in the field of behavioral pharmacology (6,7). On the other hand, the nucleus raphe magnus and the pontine reticular formation play an important role in the expression of MCL and CL, respectively (8,9).…”

Section: Introductionmentioning

confidence: 99%

Effects of Antiepileptics on Behavioral and Electroencephalographic Seizure Induced by Pentetrazol in Mice

et al. 2010

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Abstract. The present study was undertaken to investigate changes of the electroencephalogram (EEG) induced by pentetrazol (PTZ) in comparison with behavioral seizures in mice. Under pentobarbital anesthesia, mice were fixed to a stereotaxic apparatus, and electrodes were implanted into the frontal and occipital cortex. Behavioral and EEG changes were observed for 30 min following PTZ administration. After PTZ administration, mice showed myoclonic seizure (MCL) and clonic seizure (CL) in order. At the same time, spiking activity and spike-wave discharge in the cortex were observed. Phenobarbital, sodium valproate, diazepam, ethosuximide, and gabapentin caused a dose-dependent shortening of the duration of MCL and CL. In addition, they shortened the duration of spiking activity and spike-wave discharge dose-dependently. Moreover, phenytoin significantly inhibited the duration of spiking activity. It can be concluded that PTZinduced spiking activity and spike-wave discharge serve as useful indices to assess the potential of antiepileptic activity in absence and MCLs in humans. Moreover, it is supposed that employing an index of EEG activity in addition to that of behavioral activity is desirable for objectivity.

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No effect of 50 Hz magnetic field observed in a pilot study on pentylenetetrazol-induced seizures and mortality in mice

Cited by 16 publications

References 39 publications

Investigation of the effects of magnetic field exposure on febrile seizure latency, seizure duration, and electroencephalographic recordings in a rat febrile convulsion model

Investigation of the effects of magnetic field exposure on febrile seizure latency, seizure duration, and electroencephalographic recordings in a rat febrile convulsion model

Pentylenetetrazol-induced seizures are not altered by pre- or post-drug exposure to a 50 Hz magnetic field

Effects of Antiepileptics on Behavioral and Electroencephalographic Seizure Induced by Pentetrazol in Mice

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