NMR Studies of Molecules Oriented in the Nematic Phase of Liquid Crystals

Diehl, P.; Khetrapal, C. L.

doi:10.1007/978-3-662-12600-4_1

Cited by 60 publications

(40 citation statements)

References 120 publications

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“…The theory underlying dipolar and other anisotropic interactions has been described in several places, including in the early liquid crystal applications [15][16][17][18][19][20][21][22][23][24][25] as well as in more recent reviews that deal specifically with biomolecular applications. [26][27][28][29][30][31][32] Here, we provide a brief description of the basic underpinnings of the methodology, with an emphasis on its application to nucleic acids.…”

Section: Residual Dipolar Couplingsmentioning

confidence: 99%

Review NMR studies of RNA dynamics and structural plasticity using NMR residual dipolar couplings

et al. 2007

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An increasing number of RNAs are being discovered that perform their functions by undergoing large changes in conformation in response to a variety of cellular signals, including recognition of proteins and small molecular targets, changes in temperature, and RNA synthesis itself. The measurement of NMR residual dipolar couplings (RDCs) in partially aligned systems is providing new insights into the structural plasticity of RNA through combined characterization of large‐amplitude collective helix motions and local flexibility in noncanonical regions over a wide window of biologically relevant timescales (<milliseconds). Here, we review RDC methodology for studying RNA structural dynamics and survey what has been learnt thus far from application of these methods. Future methodological challenges are also identified. © 2007 Wiley Periodicals, Inc. Biopolymers 86: 384–402, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: Residual Dipolar Couplingsmentioning

confidence: 99%

Review NMR studies of RNA dynamics and structural plasticity using NMR residual dipolar couplings

et al. 2007

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“…There are a variety of NMR techniques, which provide these informations. Proton-proton dipolar couplings have been one of the major sources of information for the study of small molecules up to about ten spins oriented in liquid crystalline media [3]. However, for larger molecules and for the liquid crystals themselves the method has been of very limited use.…”

Section: Introductionmentioning

confidence: 99%

Ordering in nematic liquid crystals from NMR cross-polarization studies

Ramanathan

Sinha

2003

Pramana - J Phys

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Abstract. The measurement of dipolar couplings between nuclei is a convenient way of obtatining directly liquid crystalline ordering through NMR since the coupling is dependent on the average orientation of the dipolar vector in the magnetic field which also aligns the liquid crystal. However, measurement of the dipolar coupling between a pair of selected nuclei is beset with problems that require special solutions. In this article the use of cross polarization for measuring dipolar couplings in liquid crystals is illustrated. Transient oscillations observed during cross polarization provide the dipolar couplings between essentially isolated nearest neighbour spins which can be extracted for several sites simultaneously by employing two-dimensional NMR techniques. The use of the method for obtaining heteronuclear dipolar couplings and hence the order parameters of liquid crystals is presented. Several modifications to the basic experiment are considered and their utility illustrated. A method for obtaining proton-proton dipolar couplings, by utilizing cross polarization from the dipolar reservoir, is also presented.

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“…The proton spectra give information about the relative proton positions via the interproton dipolar couplings (10,11). These cubane derivatives have C3 symmetry and consequently each requires one order parameter to describe its preferred alignment in the magnetic field of the nmr spectrometer.…”

Section: Introductionmentioning

confidence: 99%

The structure of cubane mono and 1,4-dicarboxylic acids in lyotropic liquid crystalline solution

Tracey¹

1984

Can. J. Chem.

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ALAN S. TRACEY. Can. J. Chem. 62, 101 (1984). Proton nmr studies of cubane mono and 1,4-dicarboxylic acids in lyotropic liquid crystalline solution show that these molecules are distorted from the cubane structure. The protons of cubane dicarboxylate which are vicinal to their respective carboxylate group are displaced towards those groups along the C 3 axis resulting in an elongation relative to a cube. In cubane monocarboxylate there is also a displacement of vicinal protons toward the carboxyl group, but this is accompanied by a displacement of the protons one carbon further removed, away from the carboxyl groups. The shape of this latter compound is comparable to that of a section of a cone with the narrow end of the cone defining the proton positions adjacent to the carboxyl group while the wide end of the cone defines the positions of the adjacent set of protons. The remaining proton of course sits on the C 3 axis which determines the centre of symmetry of the cone. ALAN S. TRACEY. Can. J. Chem. 62, 101 (1984). Les Ctudes en rmn du proton des acides mono et dicarboxyliques-1.4 du cubane dans un cristal liquide lyotrope rkvklent que ces molCcules sont dtformCes par rapport a la structure du cubane. Les protons du dicarboxylate de cubane qui sont vicinaux avec leur groupe carboxylate respectif se dCplacent vers les groupes le long de I'axe C , provoquant une elongation par rapport au cubane. Dans le monocarboxylate de cubane, il y a tgalement un deplacement des protons vicinaux vers le groupe carboxyle; mais ce dCplacement des protons vicinaux s'accompagne aussi d'un dCplacement des protons qui sont sur la position suivante par rapport aux groupes carboxyles. La forme de ce dernier composC est comparable a celle d'une section d'un cane avec I'extrCmitC Ctroite du cbne dkfinissant la position du proton adjacent au groupe carboxyle tandis que I'extrtmitC plus large du cbne dCfinit les positions des ensembles de protons adjacents. Le proton restant se situe naturellement sur l'axe C 3 qui dktermine le centre de symCtrie du cbne.[ (cu-

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NMR Studies of Molecules Oriented in the Nematic Phase of Liquid Crystals

Cited by 60 publications

References 120 publications

Review NMR studies of RNA dynamics and structural plasticity using NMR residual dipolar couplings

Review NMR studies of RNA dynamics and structural plasticity using NMR residual dipolar couplings

Ordering in nematic liquid crystals from NMR cross-polarization studies

The structure of cubane mono and 1,4-dicarboxylic acids in lyotropic liquid crystalline solution

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