NMR-Based Metabonomic Study Reveals Intervention Effects of Polydatin on Potassium Oxonate-Induced Hyperuricemia in Rats

Abstract

<jats:p>Previous studies have disclosed the antihyperuricemic effect of polydatin, a natural precursor of resveratrol; however, the mechanisms of action still remain elusive. The present study was undertaken to evaluate the therapeutic effects and the underlying mechanisms of polydatin on potassium oxonate-induced hyperuricemia in rats through metabonomic technology from a holistic view. Nuclear magnetic resonance (NMR) spectroscopy was applied to capture the metabolic changes in sera and urine collected from rats induced by hyperuricemia and polydatin treatment. With multivariate data analysis, significant metabolic perturbations were observed in hyperuricemic rats compared with the healthy controls. A total of eleven and six metabolites were identified as differential metabolites related to hyperuricemia in serum and urine of rats, respectively. The proposed pathways primarily included branched-chain amino acid (BCAA) metabolism, glycolysis, the tricarboxylic acid cycle, synthesis and degradation of ketone bodies, purine metabolism, and intestinal microflora metabolism. Additionally, some metabolites indicated the risk of renal injury induced by hyperuricemia. Polydatin significantly lowered the levels of serum uric acid, creatinine, and blood urea nitrogen and alleviated the abnormal metabolic status in hyperuricemic rats by partially restoring the balance of the perturbed metabolic pathways. Our findings shed light on the understanding of the pathophysiological process of hyperuricemia and provided a reference for revealing the metabolic mechanism produced by polydatin in the treatment of hyperuricemia.</jats:p>

Polydatin Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting Ferroptosis

“…Polydatin (PD, C 20 H 22 O 8 ) is a natural active ingredient extracted from the dried roots of the perennial herb Polygonum cuspidatum Sieb. et Zucc., which might play a potential therapeutic role in various kidney disorders, such as AKI [7][8][9][10][11][12][13][14][15][16], diabetic nephropathy [17][18][19][20][21][22][23][24], lupus nephritis [25], and hyperuricemia [26][27][28], and has been used to treat patients with irritable bowel syndrome in Europe [29]; moreover, PD has been shown to exhibit mechanisms to antioxidant stress, anti-inflammation, and antifibrosis characteristics; improvement of mitochondrial damage; and regulation of autophagy [9,30,31]. Resveratrol, an aglycone of PD, has been verified to ameliorate myocardial and liver damage caused by iron overload [32][33][34]; moreover, it has been confirmed that resveratrol can reduce iron load in hemodialysis patients in clinical studies [35].…”

Carvacrol Alleviates Hyperuricemia-Induced Oxidative Stress and Inflammation by Modulating the NLRP3/NF-κB Pathwayt

“… 61 Here, we demonstrate the renal safety of carvacrol that was not previously observed with other natural compounds, such as polydatin, which decreases the serum uric acid level but increases the serum creatinine level, thereby indicating abnormal metabolic status in hyperuricemic rats. 62 In addition, carvacrol exhibited a relative safety profile because no impairment was observed in the kidneys. The serum creatinine and urea levels were also normal and similar to previous findings.…”

The Interaction Between Dietary Fructose and Gut Microbiota in Hyperuricemia and Gout

“…For example, the reduction of Bacteroides and Bifidobacteria found in the HUA rat model can be subsequently restored by supplementing with tuna oligopeptides (TMOP). Furthermore, after transplanting the fecal microbiota of TMOP-treated mice, the phenotypes of hyperuricemia and renal inflammation were attenuated, indicating that the beneficial effects of TMOP on HUA are at least partially mediated by intestinal microbes ( 163 ). In addition, prebiotics can also reduce chronic inflammation in the host by regulating the intestinal microbiota, thereby alleviating hyperuricemia.…”