NMR-based metabolomics revealed metabolic changes in energy production for viral replication and immunological response in rock bream (Oplegnathus fasciatus) tissues during rock bream iridovirus (RBIV) infection

Kim, Ahran; Yoon, Dahye; Kim, Seonghye; Ma, Seohee; Lee, Wonho; Kim, Do-Hyung; Kim, Heui‐Soo; Cha, Hee‐Jae; Choi, Yung Hyun; Kim, Suhkmann

doi:10.1016/j.aquaculture.2021.737451

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…The metabolites were identified and quantified based on chemical shift and the Biological Magnetic Resonance Data Bank in addition to other literature references (Lee et al, 2022). Data sets were conducted in a multivariate statistical analysis using MetaboAnalyst 5.0 software (Cui et al, 2022).…”

Section: Nmr Data Processing and Analysesmentioning

confidence: 99%

NMR-based metabolic analysis of Bacillus velezensis DZ11 applied to low-salt fermented coarse fish involved in the formation of flavor precursors

Zhou

Xiao-yan

et al. 2023

Food Sci. Technol

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Microbial fermentation can be responsible for flavor production and increasing the nutritional quality of fermented fish foods. The study aimed to isolate non-halophilic bacteria possessing proteolysis and lipolysis properties as starter cultures for low-salt content fermentation of coarse fish. The strains with high enzyme activities were screened and multiple growth characteristics were analyzed. The content of amino acid nitrogen and trichloroacetic acid (TCA)-soluble peptides in fermented fish was also investigated under different fermentation conditions. Nuclear magnetic resonance (NMR) metabolomics analysis was utilized to monitor the metabolite profile of fermented fish paste. The isolate was identified as Bacillus velezensis DZ11. The strain DZ11 exhibited obvious hydrolysis properties of lipids and proteins. During the fermentation of fish paste, a total of 40 differential metabolites were identified, of which multiple metabolites changed significantly, including nutrient-related substances and flavor precursors. Salt concentration and strain DZ11 had important effects on the differential metabolites. B. velezensis DZ11 under longer fermentation time promoted the formation of flavor precursors and potentially increased the nutritional substances of low-salt fermented fish.

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Section: Nmr Data Processing and Analysesmentioning

confidence: 99%

NMR-based metabolic analysis of Bacillus velezensis DZ11 applied to low-salt fermented coarse fish involved in the formation of flavor precursors

Zhou

Xiao-yan

et al. 2023

Food Sci. Technol

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“…The metabolome of the article profiling revealed several key protective metabolites against VHSV infection, including the activation of the immune system and protein synthesis, and suppression of ATP synthesis and the antioxidant system ( 16 ). The metabolic profiles of rock bream in response to rock bream iridovirus (RBIV) infection were systematically studied, revealing an elaborated mechanism of the host-pathogen interaction ( 17 ). These studies unfolded key metabolites and pathways against infectious diseases, which provided a foundational understanding of infection mechanisms and improved protection strategies of fish against viral diseases.…”

Section: Introductionmentioning

confidence: 99%

Untargeted LC-MS metabolomics reveals the metabolic responses in olive flounder subjected to hirame rhabdovirus infection

Gu,

Pan,

Wang

et al. 2023

Front. Immunol.

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Hirame novirhabdovirus (HIRRV), which mainly infects the olive flounder (Paralichthys olivaceus), is considered to be one of the most serious viral pathogens threatening the global fish culture industry. However, little is known about the mechanism of host-pathogen interactions at the metabolomic level. In this study, in order to explore the metabolic response of olive flounder to HIRRV infection, liquid chromatography mass spectrometry (LC-MS) was used to detect the changes of endogenous compounds of the olive flounder after HIRRV infection. A total of 954 unique masses were obtained, including 495 metabolites and 459 lipids. Among them, 7 and 173 qualified differential metabolites were identified at 2 days and 7 days post-infection, respectively. Distinct metabolic profiles were observed along with viral infection. At the early stage of infection, only a few metabolites were perturbed. Among them, the level of inosine and carnosine were increased and the potential antiviral ability of these two metabolites was further confirmed by exogenous addition experiment. At the late stage of HIRRV infection, the metabolic profiles changed remarkably. The changes in amino acids and nucleotides especially the 7-methylguanine also accelerated the amplification of viral particles. And the down-regulation of glutathione (GSH) implied an elevated level of ROS (reactive oxygen species) that attenuated the immune system of flounders. HIRRV also induced the accumulation of purine and reduction of pyrimidine, and elevated LPC and LPE levels. The unbalanced purine/pyrimidine and altered lipid profile may be beneficial for the replication and infection of HIRRV at the late stage of infection. These findings provide new insights into the pathogenic mechanism of HIRRV infection in olive flounder.

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Metabolomics in rare minnow (Gobiocypris rarus) after infection by attenuated and virulent grass carp reovirus genotype Ⅱ

Zhou

et al. 2023

Fish & Shellfish Immunology

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NMR-based metabolomics revealed metabolic changes in energy production for viral replication and immunological response in rock bream (Oplegnathus fasciatus) tissues during rock bream iridovirus (RBIV) infection

Cited by 3 publications

References 54 publications

NMR-based metabolic analysis of Bacillus velezensis DZ11 applied to low-salt fermented coarse fish involved in the formation of flavor precursors

NMR-based metabolic analysis of Bacillus velezensis DZ11 applied to low-salt fermented coarse fish involved in the formation of flavor precursors

Untargeted LC-MS metabolomics reveals the metabolic responses in olive flounder subjected to hirame rhabdovirus infection

Metabolomics in rare minnow (Gobiocypris rarus) after infection by attenuated and virulent grass carp reovirus genotype Ⅱ

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