Metabolomics in rare minnow (Gobiocypris rarus) after infection by attenuated and virulent grass carp reovirus genotype Ⅱ

Transcriptomics in Rare Minnow (Gobiocypris rarus) towards Attenuated and Virulent Grass Carp Reovirus Genotype II Infection

“…In this study, after virulent DY197 infection, the mRNA expression levels of tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), tumor necrosis factor superfamily member 10 (TNFSF10), caspase-7, caspase-8, BAX genes were significantly upregulated in the liver, and the mRNA expression levels of caspase7 and BAX genes were significantly upregulated in the spleen; while the mRNA expression levels of these genes were slightly upregulated after attenuated QJ205 infection in both liver and spleen. In addition, the steroid synthesis pathway associated with membrane formation was suppressed in both spleen and liver after the virulent DY197 infection, which was consistent with the virulent DY197 infection that caused severe cell necrosis and tissue damage observed by histological examination [27]. Interestingly, the steroid synthesis pathway was activated in the spleen of attenuated QJ205 infected rare minnow, which may indicate that the spleen had passed the peak of antiviral response and began to synthesize steroids to restore membrane formation at 5 dpi.…”

“…The infection of QJ205 caused slightly muscular hemorrhage symptoms and 5% mortality in rare minnow, associated with low virus copy numbers and no obvious pathological changes in the spleen and liver. In contrast, DY197 infection led to severe muscular hemorrhage symptoms and 95% mortality in rare minnow, as well as approximately 100-fold virus copy numbers of those infected with attenuated QJ205 and severe cell necrosis in the spleen and liver [27]. To further dissect the virulence-specific and tissue-specific molecular mechanism, in the present study, we performed comparative transcriptome analysis in the spleen and liver of rare minnow after virulent and attenuated isolate infection.…”