NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research

Zhang, Limin; Hatzakis, Emmanuel; Patterson, Andrew D.

doi:10.1007/s40495-016-0067-9

Cited by 16 publications

(20 citation statements)

References 93 publications

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“…In addition, NMR is a very useful technique for the structure elucidation of novel and/or unexpected compounds including those with identical masses and/or different isotopomer distributions [12,113]. Moreover, NMR-based methods are highly reproducible, noninvasive, nondestructive, and require minimal sample preparation as the sample does not get in physical contact with the device as in MS [114]. Another major strength in NMR spectroscopy lies in the direct proportionality between the NMR spectrum signals and the corresponding real molar levels of the detected metabolites, making absolute quantification of all detected metabolites possible without the need for calibration curves of individual analytes, posing it as a powerful tool in quality control purposes of drug extracts [115].…”

Section: Nuclear Magnetic Resonance (Nmr)mentioning

confidence: 99%

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Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

et al. 2020

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Plant-derived natural products have long been considered a valuable source of lead compounds for drug development. Natural extracts are usually composed of hundreds to thousands of metabolites, whereby the bioactivity of natural extracts can be represented by synergism between several metabolites. However, isolating every single compound from a natural extract is not always possible due to the complex chemistry and presence of most secondary metabolites at very low levels. Metabolomics has emerged in recent years as an indispensable tool for the analysis of thousands of metabolites from crude natural extracts, leading to a paradigm shift in natural products drug research. Analytical methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) are used to comprehensively annotate the constituents of plant natural products for screening, drug discovery as well as for quality control purposes such as those required for phytomedicine. In this review, the current advancements in plant sample preparation, sample measurements, and data analysis are presented alongside a few case studies of the successful applications of these processes in plant natural product drug discovery.

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Section: Nuclear Magnetic Resonance (Nmr)mentioning

confidence: 99%

“…Indeed, as described below, LC-online-NMR has been proposed as a route toward improving coverage of the metabolome. However, despite the advocacy of this approach, relatively few examples of its utility have been published to date [106,114].…”

Section: Nmrshiftdbmentioning

confidence: 99%

Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

et al. 2020

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“…Metabolomics, along with other ''Omics" platforms such as genomics, transcriptomics and proteomics, can complete the systemic view of biology in different research areas. 16 Metabolomics is defined as an informative analytical tool for metabolites measurement in both quantitative and qualitative conditions. 17 Fig.…”

Section: Metabolomicsmentioning

confidence: 99%

“…3 Low sensitivity and detection of limited mass range are regarded as the limitation of NMR. The general process of NMR-based metabolomics includes the following steps 16 Experimental design Sample preparation NMR raw data Fig. 2.…”

Section: Nmr (Nuclear Magnetic Resonance)mentioning

confidence: 99%

Metabolomics-based studies in the field ofLeishmania/leishmaniasis

Atan

Koushki

Ahmadi

et al. 2018

Alexandria Journal of Medicine

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a b s t r a c tLeishmania is a neglected protozoan parasite which creates some problems for public health with different clinical infections in different countries around. Due to the lack of an effective drug without side effects and the emergence drug resistance, there is an urgent need to introduce the novel drug targets and new drugs and vaccines to control leishmaniasis during recent years, metabolomics and other ''Omics" platforms has become an important approach to comprehensive knowledge of the Leishmania parasites biology. The study of metabolite profiles can open the insights for discovering novel therapeutic targets in this infection in both of the parasites and human host. In addition, specifying the metabolomics profile changes among promastigotes, amastigotes and during metacyclogenesis can pay the way for achieving parasite survival parameters and the host-parasite interaction. The previous studies in this field have been extracted from the databases, literature and their detailed major concepts. The present review highlights the role of metabolomics approach in the field of Leishmania research. Also, several important metabolite signatures introduced in various aspect of leishmania parasite such as drug resistance and parasite biology which would be useful in the field of biomarker and drug discovery process. Finally, metabolomics plays a potential role in introducing metabolic pathways related to Leishmania parasite and its treatment design. Ó 2018 Alexandria University Faculty of Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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“…Metabolomik bietet auch einen preisgünstigen und produktiven Weg zur Entdeckung neuer Zielstrukturen für die Wirkstoffsuche und zur Vorhersage und Verfolgung individueller Reaktionen auf eine medikamentöse Behandlung (Pharmakometabolomik) . Für solche Fragestellungen sind Tiermodelle die bevorzugte Wahl, vor allem für erste explorative Untersuchungen.…”

Section: Biomedizinische Und Biochemische Signifikanzunclassified

Hochdurchsatz‐Metabolomik mit 1D‐NMR

et al. 2018

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Metabolomik befasst sich mit der Gesamtheit aller Metabolite, dem Metabolom. Als eine der “Omics”‐Wissenschaften hat sie Querbeziehungen zur Biologie, Physiologie, Pathologie und Medizin; andererseits sind Metabolite chemische Verbindungen, kleine organische Moleküle oder anorganische Ionen. Ihre korrekte Identifizierung und Quantifizierung in komplexen biologischen Matrizen erfordert daher eine solide chemische Grundlage. Im Vergleich beispielsweise zu DNA unterliegen Metabolite sehr viel stärker der Oxidation oder dem enzymatischen Abbau: Wir können große Teile eines Mammut‐Genoms aus einer kleinen Probe rekonstruieren, sind aber nicht in der Lage, dasselbe mit seinem Metabolom zu tun, das innerhalb weniger Stunden nach dem Tod des Tieres wahrscheinlich weitgehend abgebaut war. Erforderlich sind daher Standardverfahren, gute chemische Fertigkeiten der Probenvorbereitung für die Lagerung und anschließende Analyse, genaue analytische Protokolle, eine umfangreiche Kenntnis der Chemometrie, erweiterte statistische Verfahren und fundiertes Wissen über mindestens eine der beiden Metabolomik‐typischen Verfahren, MS oder NMR. All diese Fertigkeiten besitzen traditionell die Chemiker. Entsprechend betrachten wir die Metabolomik aus chemischer Perspektive und beschränken uns auf NMR. Der Analytiker findet Argumente für und gegen NMR, jedoch bietet das Verfahren eine besondere ganzheitliche Perspektive, die für künftige Anwendungen als eine bevölkerungsweite Screeningtechnik für Reihenuntersuchungen sprechen.

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NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research

Cited by 16 publications

References 93 publications

Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

Metabolomics-based studies in the field ofLeishmania/leishmaniasis

Hochdurchsatz‐Metabolomik mit 1D‐NMR

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