NMR-Based Metabolomic Profiling of Mungbean Infected with Mungbean Yellow Mosaic India Virus

Maravi, Devendra Kumar; Kumar, Sanjeev; Sahoo, Lingaraj

doi:10.1007/s12010-022-04074-5

Cited by 7 publications

(12 citation statements)

References 36 publications

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“…To extract metabolites from the AWF and LWA, 1 mL AWF were snap frozen using liquid nitrogen, lyophilized, and dried. The AWF sample was then resuspended in an equal volume of aqueous methanol solution (80% v/v) and vortexed for 20 seconds (Maravi et al, 2022a). The sample was centrifuged at 12000 x g for 30 minutes at 4 °C, and the supernatant was transferred to a fresh tube and evaporated using a SpeedVac concentrator (Eppendorf AG) at room temperature for 9 hours.…”

Section: Methodsmentioning

confidence: 99%

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Dhobale,

Sahoo

2023

Preprint

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Plant viruses exert control over the host metabolism to enhance their survival, but the specific sites where altered metabolites take effect remain enigmatic. This study focuses on the apoplastic region of symptomatic mungbean leaves infected with mungbean yellow mosaic India virus (MYMIV) to gain new insights into metabolite changes during infection. Leveraging NMR-based metabolome fingerprinting, we identified several stress-associated metabolites. Notably, proline and 2-Hydroxybutyrate were found to be up-regulated in the apoplast region, while down-regulated in the cytosol. Additionally, aspartate was found to be upregulated in the cytosolic region but absent in the apoplast. Importantly, our molecular analysis of the apoplast suggests the presence of MYMIV genomic components. Moreover, we characterized two distinct populations of extracellular vesicles (EVs) using ultracentrifugation, transmission electron microscopy, dynamic light scattering, and fluorometric assays. The data revealed alterations in the size and count of EVs, suggesting their potential role in facilitating the transport of viral components for long-distance cell-to-cell movement during infection. These findings provide valuable insights into the apoplast's role and the significance of EVs in MYMIV infection, contributing to a better understanding of virus-host interactions and potentially informing new virus defense strategies.

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Section: Methodsmentioning

confidence: 99%

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Dhobale,

Sahoo

2023

Preprint

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“…76 This method can also distinguish compounds with identical masses, including those with different isotopomer distributions, 77 and compounds that exhibit similar elution behavior in conventional chromatography. For instance, the isomers 3-Oand 5-O-caffeoylquinic acid, which are challenging to Saccharides and glycosylic portion stachyose, 34,35 rhamnose in rutin, 36 pinitol, 37 rhamnose in flavonoid, 38 raffinose, 36 gentisic acid glucoside, 36 neochlorogenic acid (3-O-caffeoylquinic acid), 33,35,38,39,43,63,64 4-O-caffeoylquinic acid, 38,63 chlorogenic acid (5-O-caffeoylquinic acid), 33,35,36,38,41,43,56,57,63,65 trans-caffeoyl derivatives, 50 trans-feruloyl derivatives, 50 trans-caftaric acid, 50 trans-fertaric acid, 50 caffeoyl esters of polyhydroxy compounds, 54 p-coumaric acid, 58 5-O-feruloyl quinic acid, 35,43 trans-cinnamic acid 50 Furano lactones limonin, 59,66 limonin glucoside 59,66,67 Glucosinolates neoglucobrassicin, 28 progoitrin, 28,30 gluconapin, 48 sinigrin 51 Alkaloids synephrine, 59,66,67 nicotine, 38 vindoline, 57 …”

Section: Potentials Of 1 H Nmr In Plant Researchmentioning

confidence: 99%

“…The levels of organic acids in the tricarboxylic acid cycle tended to be elevated after fungal pathogen infections. 36,38,58,63,109 As such, fungi have been observed to produce organic acids from sugars in stored fruits. 115 after microbial infection, leading to the accumulation of fumaric, 30 succinic, 30 and malic acid.…”

Section: Historical Overview Of the Application Of 1 H Nmr-based Plantmentioning

confidence: 99%

“…Amino acid variations upon viral infection are dependent on the host plant species and type of virus. For instance, the level of asparagine decreased in the early infection of vanilla with cymbidium mosaic virus, whereas it increased in the later stages of the disease, as in the case of mung bean infected with yellow mosaic virus . Asparagine, glutamine, and proline are stress amino acids known to increase in response to insect pathogens. , Proline, a recognized radical scavenger, contributed to maintaining cellular redox homeostasis .…”

Section: An Invaluable Tool In the Assessment Of Constitutive And Ind...mentioning

confidence: 99%

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Twenty Years of ¹H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress

Mascellani Bergo,

Leiss,

Havlik

2024

J. Agric. Food Chem.

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Metabolomics has become an important tool in elucidating the complex relationship between a plant genotype and phenotype. For over 20 years, nuclear magnetic resonance (NMR) spectroscopy has been known for its robustness, quantitative capabilities, simplicity, and cost-efficiency. 1 H NMR is the method of choice for analyzing a broad range of relatively abundant metabolites, which can be used for both capturing the plant chemical profile at one point in time and understanding the pathways that underpin plant defense. This systematic Review explores how 1 H NMR-based plant metabolomics has contributed to understanding the role of various compounds in plant responses to biotic stress, focusing on both primary and secondary metabolites. It clarifies the challenges and advantages of using 1 H NMR in plant metabolomics, interprets common trends observed, and suggests guidelines for method development and establishing standard procedures.

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“…The YMV infection modified the vital metabolic processes. Differences in the accumulation of γ-amino butyric acid (GABA), sucrose, alanine, proline, tryptophan, phenylalanine, citrates, pyruvate, and ascorbate were observed [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomic Profiling of Horse Gram (Macrotyloma uniflorum (Lam.) Verdc.) Genotypes for Horse Gram Yellow Mosaic Virus Resistance

et al. 2023

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Horse gram [Macrotyloma uniflorum Lam. (Verdc.)] is an under-utilized legume grown in India. It is a good source of protein, carbohydrates, dietary fiber, minerals, and vitamins. We screened 252 horse gram germplasm accessions for horse gram yellow mosaic virus resistance using the percent disease index and scaling techniques. The percentage values of highly resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible were 0.34, 13.89, 38.89, 46.43, and 0.34, respectively. Repetitive trials confirmed the host-plant resistance levels, and yield loss was assessed. The present disease index ranged from 1.2 to 72.0 and 1.2 to 73.0 during the kharif and rabi seasons of 2018, respectively. The maximum percent yield loss was noticed in the HS (75.0 –89.4), while HR possessed the minimum (1.2–2.0). The methanolic leaf extracts of highly resistant and highly susceptible genotypes with essential controls were subjected to gas chromatography–mass spectrometry analysis. Differential accumulation of metabolites was noticed, and a total of 81 metabolites representing 26 functional groups were identified. Both highly resistant and susceptible genotypes harbored eight unique classes, while ten biomolecules were common. The hierarchical cluster analysis indicated a distinct metabolite profile. Fold change in the common metabolites revealed an enhanced accumulation of sugars, alkanes, and carboxylic acids in the highly resistant genotype. The principal component analysis plots explained 93.7% of the variation. The metabolite profile showed a significant accumulation of three anti-viral (octadecanoic acid, diphenyl sulfone, and 2-Aminooxazole), one insecticidal (9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol), one antifeedant (cucurbitacin B), and six metabolites with unknown biological function in the highly resistant genotype.

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NMR-Based Metabolomic Profiling of Mungbean Infected with Mungbean Yellow Mosaic India Virus

Cited by 7 publications

References 36 publications

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Twenty Years of ¹H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress

Comparative Metabolomic Profiling of Horse Gram (Macrotyloma uniflorum (Lam.) Verdc.) Genotypes for Horse Gram Yellow Mosaic Virus Resistance

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NMR-Based Metabolomic Profiling of Mungbean Infected with Mungbean Yellow Mosaic India Virus

Cited by 7 publications

References 36 publications

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Twenty Years of 1H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress

Comparative Metabolomic Profiling of Horse Gram (Macrotyloma uniflorum (Lam.) Verdc.) Genotypes for Horse Gram Yellow Mosaic Virus Resistance

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Twenty Years of ¹H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress