NLIP and HAD-like Domains of Pah1 and Lipin 1 Phosphatidate Phosphatases Are Essential for Their Catalytic Activities

Hsu, Wei‐Hsin; Huang, Yihao; Chen, Pin‐Ru; Hsieh, Lu‐Sheng

doi:10.3390/molecules26185470

Cited by 11 publications

(9 citation statements)

References 53 publications

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“…Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed according to the methods published before [48,49]. Following electrophoresis, proteins on a gel were stained by Coomassie Brilliant Blue R-250 (CBR) and de-stained by 20% methanol.…”

Section: Biochemical Properties Analysismentioning

confidence: 99%

Production of Trans-Cinnamic Acid by Immobilization of the Bambusa oldhamii BoPAL1 and BoPAL2 Phenylalanine Ammonia-Lyases on Electrospun Nanofibers

Hong

Huang

Lim

et al. 2021

IJMS

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Phenylalanine ammonia-lyase (PAL) catalyzes the nonoxidative deamination of phenylalanine to yield trans-cinnamic acid and ammonia. Recombinant Bambusa oldhamii BoPAL1/2 proteins were immobilized onto electrospun nanofibers by dextran polyaldehyde as a cross-linking agent. A central composite design (CCD)-response surface methodology (RSM) was utilized to optimize the electrospinning parameters. Escherichia coli expressed eBoPAL2 exhibited the highest catalytic efficiency among four enzymes. The optimum conditions for fabricating nanofibers were determined as follows: flow rate of 0.10 mL/h, voltage of 13.8 kV, and distance of 13 cm. The response surface models were used to obtain the smaller the fiber diameters as well as the highest PAL activity in the enzyme immobilization. Compared with free BoPALs, immobilized BoPALs can be reused for at least 6 consecutive cycles. The remained activity of the immobilized BoPAL proteins after storage at 4 °C for 30 days were between 75 and 83%. In addition, the tolerance against denaturants of the immobilized BoPAL proteins were significantly enhanced. As a result, the dextran polyaldehyde natural cross-linking agent can effectively replace traditional chemical cross-linking agents for the immobilization of the BoPAL enzymes. The PAL/nylon 6/polyvinyl alcohol (PVA)/chitosan (CS) nanofibers made are extremely stable and are practical for industrial applications in the future.

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Section: Biochemical Properties Analysismentioning

confidence: 99%

Production of Trans-Cinnamic Acid by Immobilization of the Bambusa oldhamii BoPAL1 and BoPAL2 Phenylalanine Ammonia-Lyases on Electrospun Nanofibers

Hong

Huang

Lim

et al. 2021

IJMS

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“…The annotated gene TraesCS5B02G029100 encodes phosphatidate phosphatase, an enzyme that catalyzes the dephosphorylation of phosphatidate to produce diacylglycerol. This enzyme plays a crucial role in regulating phospholipid and triacylglycerol metabolism [104].…”

Section: Association With Twmentioning

confidence: 99%

Genome-Wide Association Study for Yield and Yield-Related Traits in Chinese Spring Wheat

Tian,

Liu,

Cui

et al. 2023

Agronomy

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Wheat (Triticum aestivum L.) is one of the important grain crops that fulfill global food security requirements. Understanding the genetic basis of wheat yield and related traits is crucial for increasing yield through marker-assisted selection (MAS). In this study, a phenotypic analysis was conducted on the yield and related traits of 192 Chinese spring wheat genotypes in six field environments. Based on the 90K wheat SNP iSelect assay, a genome-wide association study (GWAS) identified 84 stable and significantly associated signals at 50 loci for 8 out of the 10 analyzed traits. These traits included grain yield (1), plant height (6), spike length (21), productive spikelet rate (12), kernel number per spikelet (1), kernel number per main spike (2), thousand kernel weight (5), and test weight (2). Seventy-one stable SNP markers were mapped to annotated genes, with 51 of them located in the coding sequences (CDSs) of 47 explanatory genes. Haplotype analysis revealed three blocks on chromosome 5A and two blocks on chromosome 5D associated with plant height (PH). Varieties with different haplotypes at these loci displayed a significant difference in plant height. The performance of traits was improved by increasing the number of superior alleles for productive spikelet rate and spike length. These results provided prospective alleles for controlling yield and yield composition in wheat breeding. These alleles could be used for marker-assisted selection to improve wheat yield.

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“…E. coli cells that expressed His 6 -tagged BoPAL proteins were freshly collected or resuspended from −20 • C freezer, disrupting by sonication using a Branson cell disruptor [32,49,50]. Proteins were purified from cell lysates by affinity chromatography with Nuvia TM Ni-NTA resin.…”

Section: Preparations Of Bopal4 Enzymesmentioning

confidence: 99%

Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4

et al. 2021

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Phenylalanine ammonia-lyase (PAL) links the plant primary and secondary metabolisms, and its product, trans-cinnamic acid, is derived into thousands of diverse phenylpropanoids. Bambusa oldhamii BoPAL4 has broad substrate specificity using L-phenylalanine, L-tyrosine, and L-3,4-dihydroxy phenylalanine (L-DOPA) as substrates to yield trans-cinnamic acid, p-coumaric acid, and caffeic acid, respectively. The optimum reaction pH of BoPAL4 for three substrates was measured at 9.0, 8.5, and 9.0, respectively. The optimum reaction temperatures of BoPAL4 for three substrates were obtained at 50, 60, and 40 °C, respectively. The Km values of BoPAL4 for three substrates were 2084, 98, and 956 μM, respectively. The kcat values of BoPAL4 for three substrates were 1.44, 0.18, and 0.06 σ-1, respectively. The major substrate specificity site mutant, BoPAL4-H123F, showed better affinity toward L-phenylalanine by decreasing its Km value to 640 μM and increasing its kcat value to 1.87 s-1. In comparison to wild-type BoPAL4, the specific activities of BoPAL4-H123F using L-tyrosine and L-DOPA as substrates retained 5.4% and 17.8% residual activities. Therefore, L-phenylalanine, L-tyrosine, and L-DOPA are bona fide substrates for BoPAL4.

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NLIP and HAD-like Domains of Pah1 and Lipin 1 Phosphatidate Phosphatases Are Essential for Their Catalytic Activities

Cited by 11 publications

References 53 publications

Production of Trans-Cinnamic Acid by Immobilization of the Bambusa oldhamii BoPAL1 and BoPAL2 Phenylalanine Ammonia-Lyases on Electrospun Nanofibers

Production of Trans-Cinnamic Acid by Immobilization of the Bambusa oldhamii BoPAL1 and BoPAL2 Phenylalanine Ammonia-Lyases on Electrospun Nanofibers

Genome-Wide Association Study for Yield and Yield-Related Traits in Chinese Spring Wheat

Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4

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