Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4

Hsieh, Chun-Yen; Huang, Yihao; Yeh, Hui-Hsuan; Hong, Pei-Yu; Hsiao, Chiaolong; Hsieh, Lu‐Sheng

doi:10.3390/catal11111263

Cited by 12 publications

(19 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resulting naringenin chalcone is converted to (2 S )-naringenin by the enzyme chalcone isomerase (CHI). In literature, it has been reported that (2 S )-naringenin could be obtained from phenylalanine ammonia-lyase (PAL) using 4CL, CHS, and CHI enzymes [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Design and Assembly of a Biofactory for (2S)-Naringenin Production in Escherichia coli: Effects of Oxygen Transfer on Yield and Gene Expression

et al. 2023

View full text Add to dashboard Cite

The molecule (2S)-naringenin is a scaffold molecule with several nutraceutical properties. Currently, (2S)-naringenin is obtained through chemical synthesis and plant isolation. However, these methods have several drawbacks. Thus, heterologous biosynthesis has emerged as a viable alternative to its production. Recently, (2S)-naringenin production studies in Escherichia coli have used different tools to increase its yield up to 588 mg/L. In this study, we designed and assembled a bio-factory for (2S)-naringenin production. Firstly, we used several parametrized algorithms to identify the shortest pathway for producing (2S)-naringenin in E. coli, selecting the genes phenylalanine ammonia lipase (pal), 4-coumarate: CoA ligase (4cl), chalcone synthase (chs), and chalcone isomerase (chi) for the biosynthetic pathway. Then, we evaluated the effect of oxygen transfer on the production of (2S)-naringenin at flask (50 mL) and bench (4 L culture) scales. At the flask scale, the agitation rate varied between 50 rpm and 250 rpm. At the bench scale, the dissolved oxygen was kept constant at 5% DO (dissolved oxygen) and 40% DO, obtaining the highest (2S)-naringenin titer (3.11 ± 0.14 g/L). Using genome-scale modeling, gene expression analysis (RT-qPCR) of oxygen-sensitive genes was obtained.

show abstract

Section: Resultsmentioning

confidence: 99%

Design and Assembly of a Biofactory for (2S)-Naringenin Production in Escherichia coli: Effects of Oxygen Transfer on Yield and Gene Expression

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The enzyme antioxidant system is the first line of defense in plants that neutralizes ROS; these enzymes include SOD, CAT, APX, and glutathione peroxidase (GPX) [67,68]. There are other enzymes that are not classified as antioxidants; however, they are indirectly involved, and one of them is PAL, which catalyzes the first reaction of the phenylpropanoid pathway, compounds with high antioxidant capacities [69]. Nonenzymatic antioxidants, whether hydrophilic (phenols, GSH, and ascorbic acid) or lipophilic (carotenoids), are the second line of defense and are responsible for inhibiting the production of damage caused by the oxidative reaction [67,70].…”

Section: Enzymatic Activity Hydrophilic Antioxidants and Antioxidant ...mentioning

confidence: 99%

The Biostimulant Effect of Hydroalcoholic Extracts of Sargassum spp. in Tomato Seedlings under Salt Stress

Sariñana-Aldaco

Benavides-Mendoza

Robledo‐Olivo

et al. 2022

Plants

View full text Add to dashboard Cite

Currently, the use of biostimulants in agriculture is a tool for mitigating certain environmental stresses. Brown algae extracts have become one of the most important categories of biostimulants in agriculture, and are derived from the different uses and positive results obtained under optimal and stressful conditions. This study aimed to examine the efficacy of a foliar application of a hydroalcoholic extract of Sargassum spp. and two controls (a commercial product based on Ascophyllum nodosum and distilled water) with regard to growth, the antioxidant system, and the expression of defense genes in tomato seedlings grown in nonsaline (0 mM NaCl) and saline (100 mM NaCl) conditions. In general, the results show that the Sargassum extract increased the growth of the seedlings at the end of the experiment (7.80%) compared to the control; however, under saline conditions, it did not modify the growth. The Sargassum extract increased the diameter of the stem at the end of the experiment in unstressed conditions by 14.85% compared to its control and in stressful conditions by 16.04% compared to its control. Regarding the accumulation of total fresh biomass under unstressed conditions, the Sargassum extract increased it by 19.25% compared to its control, and the accumulation of total dry biomass increased it by 18.11% compared to its control. Under saline conditions, the total of fresh and dry biomass did not change. Enzymatic and nonenzymatic antioxidants increased with NaCl stress and the application of algal products (Sargassum and A. nodosum), which was positively related to the expression of the defense genes evaluated. Our results indicate that the use of the hydroalcoholic extract of Sargassum spp. modulated different physiological, metabolic, and molecular processes in tomato seedlings, with possible synergistic effects that increased tolerance to salinity.

show abstract

“…Bambusa oldhamii , green bamboo, is a perennial plant in the tropics and subtropics, and bamboo shoot is an economic vegetable in far-eastern Asia [ 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Bamboo is one of the fast-growth timber plants, which are controlled by plant hormones, especially cytokinins [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

Cloning of Three Cytokinin Oxidase/Dehydrogenase Genes in Bambusa oldhamii

Hsieh¹,

Hsieh

2023

CIMB

Self Cite

View full text Add to dashboard Cite

Cytokinin oxidase/dehydrogenase (CKX) catalyzes the irreversible breakdown of active cytokinins, which are a class of plant hormones that regulate cell division. According to conserved sequences of CKX genes from monocotyledons, PCR primers were designed to synthesize a probe for screening a bamboo genomic library. Cloned results of three genes encoding cytokinin oxidase were named as follows: BoCKX1, BoCKX2, and BoCKX3. In comparing the exon-intron structures among the above three genes, there are three exons and two introns in BoCKX1 and BoCKX3 genes, whereas BoCKX2 contains four exons and three introns. The amino acid sequence of BoCKX2 protein shares 78% and 79% identity with BoCKX1 and BoCKX3 proteins, respectively. BoCKX1 and BoCKX3 genes are particularly closely related given that the amino acid and nucleotide sequence identities are more than 90%. These three BoCKX proteins carried putative signal peptide sequences typical of secretion pathway, and a GHS-motif was found at N-terminal flavin adenine dinucleotide (FAD) binding domain, suggesting that BoCKX proteins might covalently conjugate with an FAD cofactor through a predicted histidine residue.

show abstract

Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4

Cited by 12 publications

References 52 publications

Design and Assembly of a Biofactory for (2S)-Naringenin Production in Escherichia coli: Effects of Oxygen Transfer on Yield and Gene Expression

Design and Assembly of a Biofactory for (2S)-Naringenin Production in Escherichia coli: Effects of Oxygen Transfer on Yield and Gene Expression

The Biostimulant Effect of Hydroalcoholic Extracts of Sargassum spp. in Tomato Seedlings under Salt Stress

Cloning of Three Cytokinin Oxidase/Dehydrogenase Genes in Bambusa oldhamii

Contact Info

Product

Resources

About