Nkx2.2 antisense RNA overexpression enhanced oligodendrocytic differentiation

Tochitani, Shiro; Hayashizaki, Yoshihide

doi:10.1016/j.bbrc.2008.05.127

Cited by 86 publications

(73 citation statements)

References 25 publications

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“…Although little information is available regarding lncRNAs in the regulation of astrocyte differentiation, forced expression of either NKX2.2 or Nkx2.2AS, an antisense ncRNA to Nkx2.2, can enhance induction of differentiation along the oligodendrocytic lineage [5]. NKX2.2 is known to be one of the TFs that direct NSCs into the oligodendrocytic lineage.…”

Section: (A) Long Non-coding Rnasmentioning

confidence: 99%

“…Meanwhile, the major effects of the core networks on their downstream gene expression through epigenetic mechanisms are now being analysed by many researchers, and noncoding RNAs (ncRNAs) are emerging as epigenetic players in embryogenesis and in developmental processes [4]. So far, most efforts aiming to understand ncRNA functions in pluripotency and neural differentiation have focused on the mouse as a model system [4][5][6][7][8]. Recent studies of human and mouse ESCs and iPSCs indicate that long ncRNAs (lncRNAs) are integral members of the ESC self-renewal regulatory circuit [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Epigenetic setting and reprogramming for neural cell fate determination and differentiation

Imamura

Uesaka

Nakashima

2014

Phil. Trans. R. Soc. B

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In the mammalian brain, epigenetic mechanisms are clearly involved in the regulation of self-renewal of neural stem cells and the derivation of their descendants, i.e. neurons, astrocytes and oligodendrocytes, according to the developmental timing and the microenvironment, the 'niche'. Interestingly, local epigenetic changes occur, concomitantly with genome-wide level changes, at a set of gene promoter regions for either down-or upregulation of the gene. In addition, intergenic regions also sensitize the availability of epigenetic modifiers, which affects gene expression through a relatively long-range chromatinic interaction with the transcription regulatory machineries including non-coding RNA (ncRNA) such as promoter-associated ncRNA and enhancer ncRNA. We show that such an epigenetic landscape in a neural cell is statically but flexibly formed together with a variable combination of generally and locally acting nuclear molecules including master transcription factors and cell-cycle regulators. We also discuss the possibility that revealing the epigenetic regulation by the local DNA -RNA -protein assemblies would promote methodological innovations, e.g. neural cell reprogramming, engineering and transplantation, to manipulate neuronal and glial cell fates for the purpose of medical use of these cells.

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Section: (A) Long Non-coding Rnasmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epigenetic setting and reprogramming for neural cell fate determination and differentiation

Imamura

Uesaka

Nakashima

2014

Phil. Trans. R. Soc. B

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“…Aberrant transcription of Nkx2.2as in Neural Stem Cell (NSC) can induce the oligodendrocyte differentiation by Nkx2.2 upregulation, indicating that Nkx2.2as regulates NSC differentiation by increasing the expression of Nkx2.2 (Tochitani and Hayashizaki, 2008).…”

Section: Regulation Of Lncrna Expression In the Nervous Systemmentioning

confidence: 99%

Long Non-coding RNAs: key players in brain and central nervous system development

Lv¹,

Liu²,

Liu³

et al. 2014

cmb

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CanadaComputational Molecular Biology (Online, ISSN 1927-5587) ), indexed by international database ProQuest, is an open access, peer reviewed journal publishing original research papers of general interest involving in the computational biology at the molecular level.The Journal is publishing all the latest and outstanding research articles, letters, methods, and reviews in all areas of Computational Molecular Biology, covering new discoveries in molecular biology, from genes to genomes, using statistical, mathematical, and computational methods as well as new development of computational methods and databases in molecular and genome biology.The papers published in the journal are expected to be of interests to computational scientists, biologists, and teachers/students/researchers engaged in biology, as well as are appropriate for R & D personnel and general readers interested in computational technology and biology.Computational Molecular Biology is published independently by BioPublisher. It is committed to publishing and disseminating significant original achievements in the related research fields of molecular biology. Vol.4, No.5, 1-13 (doi: 10.5376/cmb.2014.04.0005) Abstract Regulatory long non-coding RNAs have been emerged as a major contribution of cognitive evolution in mammalian central nervous system and brain tissues. Though proteins have relatively conserved during evolution, the lncRNAs have evolved rapidly to cope with essential and widespread cellular regulation, partly by directing generic protein function. Long non-coding RNAs, highly yet specifically expressed in mammalian brain, provide tissue-and neuronal activity-specific epigenetic and transcriptional regulation. lncRNAs have been documented to be essential for brain development and be involved in brain related diseases. We suggest that lncRNAs are important to modulate diverse central nervous system processes and are the major factor that is important to the brain development, which may be employed to develop novel diagnostic and therapeutic strategies to treat brain related diseases. Moreover, animal models with altered lncRNA expressions and high-throughput approaches would help to understand the mechanisms of lncRNAs in brain development and the etiology of lncRNA-driven human neurological diseases.

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“…lncRNAs have been shown to modulate gene transcription via different mechanisms. For example, some cis-antisense lncRNAs complementary to protein-coding transcripts regulate expression of the coding genes (Feng et al, 2006;Kotake et al, 2010;Pasmant et al, 2007;Tochitani and Hayashizaki, 2008;Yu et al, 2008); some lncRNAs modulate transcription factors by acting as co-regulators (Feng et al, 2006;Nguyen et al, 2001;Shamovsky et al, 2006;Wang et al, 2008;Willingham et al, 2005;Yang et al, 2001). Moreover, some studies have shown that lncRNAs are involved in epigenetic regulations such as chromatin and histone modification, and X-chromosome inactivation (Denisenko et al, 1998;Mancini-Dinardo et al, 2006;Mazo et al, 2007;Rinn et al, 2007;Sanchez-Elsner et al, 2006;Tsai et al, 2010;Wutz et al, 2002).…”

Section: Lncrnasmentioning

confidence: 99%

“…5C). Ectopic expression of Nkx2.2as in cultured NSCs induces oligodendrocyte differentiation through an upregulation of the Nkx2.2 mRNA level, suggesting that Nkx2.2as regulates NSC differentiation and promotes gliogenesis by modulating protein coding gene Nkx2.2 expression (Tochitani and Hayashizaki, 2008).…”

Section: Cell Fate Determination Regulated By Lncrnasmentioning

confidence: 99%