Nitrosubstituted Aromatic Compounds as Nitrogen Source for Bacteria

Bruhn, Clemens; Lenke, Hiltrud; Knackmuss, Hans‐Joachim

doi:10.1128/aem.53.1.208-210.1987

Cited by 170 publications

(53 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2B). This agrees with other published reports on nitrotoluene degradation [10, 16,30]. We observed a faster rate of degradation of 3-NT (70 pmol h-'1 under anaerobic conditions as compared to the aerobic conditions, but the amount of 3-AT accumulated in the medium was less than the estimated conversion of 3-NT (Fig.…”

Section: Biodegradation Of 3-nitrotoluenesupporting

confidence: 93%

See 1 more Smart Citation

A novel pathway for the biodegradation of 3-nitrotoluene in Pseudomonas putida

Ali-Sadat

Mohan

Walia

1995

FEMS Microbiology Ecology

View full text Add to dashboard Cite

3‐Nitrotoluene was degraded when incubated with the resting cells of Pseudomonas putida OU83. Most of the 3‐nitrotoluene (70%) was metabolized via reduction of the nitro group to form 3‐aminotoluene (3‐AT). A minor portion (30%) was degraded through a novel pathway involving oxidation of 3‐NT to form 3‐nitrophenol through a series of intermediary metabolites: 3‐nitrobenzyl alcohol, 3‐nitrobenzaldehyde and 3‐nitrobenzoic acid. Degradation of 3‐nitrophenol occurred with the formation of a transient intermediary metabolite, hydroxynitroquinone, which was further degraded with the near stoichiometric release of nitrite into the medium. 3‐Nitrotoluene‐induced cells showed increased oxygen consumption with 3‐nitrotoluene, 3‐nitrobenzaldehyde, 3‐nitrobenzoate, and 3‐nitrophenol as substrates in comparison to uninduced cells. Cell extracts prepared from strain OU83 contained benzylalcohol dehydrogenase and benzaldehyde dehydrogenase activities. The experimental evidence suggests a novel pathway for the degradation of 3‐NT in which C‐1 elimination is catalyzed by a cofactor‐independent deformylase, rather than a decarboxylase or dioxygenase.

show abstract

Section: Biodegradation Of 3-nitrotoluenesupporting

confidence: 93%

“…Nitroaromatics are toxic to algae, fish, and nitroamines. When bound to organic matter, such as higher animals [3-61; the mechanism of toxicity is humic acid, nitroamines are immobilized and diffireportedly through the uncoupling of oxidative phos-cult to degrade [10, 16,17]. Recent investigations have phorylation [5,7].…”

Section: Introductionmentioning

confidence: 99%

A novel pathway for the biodegradation of 3-nitrotoluene in Pseudomonas putida

Ali-Sadat

Mohan

Walia

1995

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…Nitroaromatic compounds are widely distributed in the environment because of their extensive use as pesticides, pharmaceuticals, dyes, plastics, explosives and solvents (Munnecke 1976;Hallas and Alexander 1983;Bruhn et al 1987;Stevens et al 1991;Spain 1995).These compounds may then enter industrial waste streams where they have deleterious consequences. Nitroaromatics and products of their incomplete degradation have relatively high acute toxicity, and some may be potential carcinogens (Chapman and Hopper 1968;Bruhn et al 1987). Nitrophenols can also build up in the soil as a result of hydrolysis of several organophosphorous insecticides, such as parathion or methylparathion, or from the use of other nitrophenolic herbicides (Munnecke 1976;Stevens et al1991;Spain1995).…”

Section: Introductionmentioning

confidence: 99%

Degradation of 4-nitrocatechol by Burkholderia cepacia: a plasmid-encoded novel pathway

2000

View full text Add to dashboard Cite

Pseudomonas cepacia RKJ200 (now described as Burkholderia cepacia) has been shown to utilize p‐nitrophenol (PNP) as sole carbon and energy source. The present work demonstrates that RKJ200 utilizes 4‐nitrocatechol (NC) as the sole source of carbon, nitrogen and energy, and is degraded with concomitant release of nitrite ions. Several lines of evidence, including thin layer chromatography, gas chromatography, 1H‐nuclear magnetic resonance, gas chromatography–mass spectrometry, spectral analyses and quantification of intermediates by high performance liquid chromatography, have shown that NC is degraded via 1,2,4‐benzenetriol (BT) and hydroquinone (HQ) formation. Studies carried out on a PNP– derivative and a PNP+ transconjugant also demonstrate that the genes for the NC degradative pathway reside on the plasmid present in RKJ200; the same plasmid had earlier been shown to encode genes for PNP degradation, which is also degraded via HQ formation. It is likely, therefore, that the same sets of genes encode the further metabolism of HQ in NC and PNP degradation.

show abstract

“…p-Nitrophenol (PNP), a priority environmental pollutant [1], occurs in industrial e¥uents [2] posing esthetic and health problems [3]. Pure cultures of aerobic soil bacteria readily metabolize PNP with removal of the nitro group as nitrite [4^8].…”

Section: Introductionmentioning

confidence: 99%

High affinity p-nitrophenol oxidation by Bacillus sphaericus JS905

Kadiyala¹

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

Bacillus sphaericus JS905, isolated from an agricultural soil by selective enrichment, transforms p‐nitrophenol (PNP) releasing only nitrite in stoichiometric amounts. The kinetics of PNP oxidation by this strain were successfully measured using an extant respirometric assay. Washed PNP‐grown cells displayed very high substrate affinity (Ks) values of 0.003 and 0.023 mg PNP l−1, and low specific growth rate (μmax) values of 0.021 and 0.019 h−1 at 30 and 37°C, respectively, at initial substrate concentration of 0.70 and 1.40 mg PNP l−1. Substrate inhibition was only evident at substrate concentration of 2.80 mg PNP l−1; the measured kinetic parameters were 0.055 and 0.043 h−1. Temperature had a small impact on the measured values for μmax and Ks. Surprisingly, values for the endogenous decay coefficient (0.015 and 0.020 h−1 at 30 and 37°C, respectively) were of the same size as the specific growth rate. This indicates that net growth of B. sphaericus JS905 on PNP when used as a sole substrate is very small. As a result, the advantage of the high affinity PNP removal by this strain must probably depend on its growth on other primary substrates. The O2/PNP stoichiometry indicates the consumption of 3 mol of molecular oxygen for the oxidation of each mol of PNP.

show abstract

Nitrosubstituted Aromatic Compounds as Nitrogen Source for Bacteria

Cited by 170 publications

References 18 publications

A novel pathway for the biodegradation of 3-nitrotoluene in Pseudomonas putida

A novel pathway for the biodegradation of 3-nitrotoluene in Pseudomonas putida

Degradation of 4-nitrocatechol by Burkholderia cepacia: a plasmid-encoded novel pathway

High affinity p-nitrophenol oxidation by Bacillus sphaericus JS905

Contact Info

Product

Resources

About