1995
DOI: 10.1111/j.1574-6941.1995.tb00140.x
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A novel pathway for the biodegradation of 3-nitrotoluene in Pseudomonas putida

Abstract: 3‐Nitrotoluene was degraded when incubated with the resting cells of Pseudomonas putida OU83. Most of the 3‐nitrotoluene (70%) was metabolized via reduction of the nitro group to form 3‐aminotoluene (3‐AT). A minor portion (30%) was degraded through a novel pathway involving oxidation of 3‐NT to form 3‐nitrophenol through a series of intermediary metabolites: 3‐nitrobenzyl alcohol, 3‐nitrobenzaldehyde and 3‐nitrobenzoic acid. Degradation of 3‐nitrophenol occurred with the formation of a transient intermediary … Show more

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Cited by 20 publications
(5 citation statements)
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“…Corresponding observations for the TOL-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase have been reported by Delgado et al (7). Recent investigations have demonstrated reductive as well as oxidative initial attack during 3-NT metabolism in Pseudomonas putida OU83 (1). While 70% of the substrate was reduced to 3-aminotoluene, a minor part (30%) was converted, via 3-nitrobenzylalcohol, 3-nitrobenzaldehyde, and 3-nitrobenzoic acid, to 3-nitrophenol.…”
supporting
confidence: 70%
“…Corresponding observations for the TOL-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase have been reported by Delgado et al (7). Recent investigations have demonstrated reductive as well as oxidative initial attack during 3-NT metabolism in Pseudomonas putida OU83 (1). While 70% of the substrate was reduced to 3-aminotoluene, a minor part (30%) was converted, via 3-nitrobenzylalcohol, 3-nitrobenzaldehyde, and 3-nitrobenzoic acid, to 3-nitrophenol.…”
supporting
confidence: 70%
“…Since there are various ways by which enzymes might become available for recruitment, more than one novel pathway might be patched together from promiscuous enzymes in a single bacterium. Indeed, Pseudmonas putida OU83 transforms 3-nitrotoluene via two routes25; 70% is reduced to 3-aminotoluene, and 30% is converted to 3-nitrophenol, from which the nitro group is removed by an unidentified process.…”
Section: Recruitment Of Promiscuous Enzymes To Serve New Functionsmentioning
confidence: 99%
“…Furthermore, the solvent tolerance of P. putida DOT-T1E might be exploited to enlarge the spectrum of solvents applicable for in situ product extraction and for the production of a broader range of organic compounds in two-liquid-phase bioreactor setups (11). m-Nitrotoluene was not oxidized in P. putida strains DOT-T1E and PpS81, yet the synthesis of oxidative enzymes involved in the mineralization of m-nitrotoluene via m-nitrobenzyl alcohol, m-nitrobenzaldehyde, and mnitrobenzoic acid has been reported for P. putida strain OU83 upon induction with m-nitrotoluene (1,50). Interestingly, mtoluidine accumulated as the main product, apparently without significant inhibition of the m-nitrotoluene hydroxylation.…”
Section: Discussionmentioning
confidence: 99%
“…Their electrophilic character makes them susceptible to reduction by all kinds of microbial systems (41). Numerous bacterial strains, such as those of Pseudomonas species (1,43) and E. coli (13), provide enzymes that are able to transform nitro groups of aromatic compounds under aerobic conditions. This indicates that biocatalysis of nitroaromatics might be complicated by influences of the enzymatic background activity of the host strain (52).…”
mentioning
confidence: 99%