Nitrosative stress in the brain: Autoantibodies to nitrotyrosine in the liquor as a potential marker

Онуфриев, М. В.

doi:10.1134/s1819712410030116

Cited by 3 publications

(2 citation statements)

References 97 publications

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“…During the remitting phase, peroxynitrite participates in neuron and oligodendrocyte damage associated with inflammatory processes. Moreover, in the chronic phase of the disease, peroxynitrite contributes to self-perpetuating mechanisms responsible for its progression [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

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The Level of Isoprostanes as a Non-invasive Marker for in vivo Lipid Peroxidation in Secondary Progressive Multiple Sclerosis

Miller¹,

Mrowicka

Saluk-Juszczak

et al. 2011

Neurochem Res

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Oxidative stress leads to lipid peroxidation and may contribute to the pathogenesis of lesions in multiple sclerosis (MS), an autoimmune disease characterized by inflammatory as well as degenerative phenomena. Isoprostanes are prostaglandin-like compounds which are formed by free radical catalysed peroxidation of arachidonic acid esterified in membrane phospholipids. They are a new class of sensitive specific markers for in vivo lipid peroxidation. In this study 26 patients (15 females and 11 males; mean age 48.2 ± 15.2 year; mean disease duration 10.0 ± 6.5 year) with secondary progressive MS (SPMS) and 12 healthy controls were enrolled. In patients with multiple sclerosis the lipid peroxidation as the level of urine isoprostanes and the level of thiobarbituric acid reactive species (TBARS) in plasma were estimated. Moreover, we estimated the total antioxidative status (TAS) in plasma. It was found that the urine isoprostanes level was over 6-fold elevated in patients with SPMS than in control (P < 0.001). In SPMS patients TBARS level was also statistically higher than in controls (P < 0.01). However, we did not observed any difference of TAS level in serum between SPMS patients and controls (P > 0.05). In patients with SPMS the lipid peroxidation and oxidative stress measured as the increased level of isoprostanes was observed. Thus, we suggest that the level of isoprostanes may be used as non-invasive marker for a determination of oxidative stress what in turn, together with clinical symptoms, may determine an specific antioxidative therapy in SPMS patients.

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Section: Discussionmentioning

confidence: 99%

“…Then, the elevated level of 8-epi-prostaglandin (PG)-F2α reflects free radicals-induced lipid peroxidation and oxidative stress. The source of 8-epi-prostaglandin (PG)-F2α may be oxidized arachidonic acid derived not only from neuron membranes but also from membrane of different cells including erythrocytes [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

The Level of Isoprostanes as a Non-invasive Marker for in vivo Lipid Peroxidation in Secondary Progressive Multiple Sclerosis

Miller¹,

Mrowicka

Saluk-Juszczak

et al. 2011

Neurochem Res

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Enzyme immunoassay for detection of protein-bound nitrotyrosine in brain tissue and cerebrospinal fluid: Methodological issues

et al. 2012

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Action of Gaseous Nitric Oxide on Some Physical and Chemical Parameters of Human Blood Samples

Мартусевич¹,

Соловьева²,

Peretyagin³

et al. 2014

JBiSE

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We studied the metabolic changes induced by gaseous nitric oxide in whole blood samples in vitro. Blood samples were collected from healthy donors (Nizhny Novgorod station of blood transfusion). We carried out the direct bubbling of blood samples (n = 14) with gaseous flow with NO in a special appliance. We modeled standard conditions using the apparatus "Plazon" (concentration NO 800 mcg/l). Middle power of gas flow was used. The blood sparging time was 2 min, and exposition time lasted 3 min. Every blood sample volume was 5 ml. All the parameters were controlled before and after blood processing with NO. We tested lactate dehydrogenase activity in direct and reverse reactions spectrometrically by G. A. Kochetov's method. Aldehyde dehydrogenase activity was examined by B. M. Kershnhots's and E. V. Serkina's methods, superoxide dismutase-by T. V. Sirota's technology. Total protein level was examined by modified Louri's method. The concentration of lactate was tested with the automatic analyzer "SuperGL Ambulance". The indices of acidbase balance and blood gases partial pressure were estimated with special analyzer "ABL-77". Additional control of energy metabolism changes was accomplished with derivative parameters, such as coefficient of energy reaction balance and coefficient of substrate provision. Different changes of blood physical and chemical parameters are induced by NO-processing which was fixed in our experiments. There is an inhibition of erythrocytes energy metabolism, decreasing of plasma antioxidant reserves, moderate ionic disorders and of acid-base misbalance in blood samples in vitro. Besides, according to the indirect signs, the used regimen of NO-processing mainly affected erythrocytes, and stipulated methemoglobin formation. These data testify that the used dose of gaseous nitric oxide is too high for investigated human blood. In our opinion, registered negative effects of free NO may be eliminated by bound nitric oxide use (first of all in its natural form-dinitrosyliron complexes).A. K. Martusevich et al. 676

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Nitrosative stress in the brain: Autoantibodies to nitrotyrosine in the liquor as a potential marker

Cited by 3 publications

References 97 publications

The Level of Isoprostanes as a Non-invasive Marker for in vivo Lipid Peroxidation in Secondary Progressive Multiple Sclerosis

The Level of Isoprostanes as a Non-invasive Marker for in vivo Lipid Peroxidation in Secondary Progressive Multiple Sclerosis

Enzyme immunoassay for detection of protein-bound nitrotyrosine in brain tissue and cerebrospinal fluid: Methodological issues

Action of Gaseous Nitric Oxide on Some Physical and Chemical Parameters of Human Blood Samples

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