Nitrogenous Compounds from the Antarctic Fungus Pseudogymnoascus sp. HSX2#-11

Abstract

The species Pseudogymnoascus is known as a psychrophilic pathogenic fungus which is ubiquitously distributed in Antarctica. While the studies of its secondary metabolites are infrequent. Systematic research of the metabolites of the Antarctic fungus Pseudogymnoascus sp. HSX2#-11 led to the isolation of one new pyridine derivative, 4-(2-methoxycarbonyl-ethyl)-pyridine-2-carboxylic acid methyl ester (1), together with one pyrimidine, thymine (2), and eight diketopiperazines, cyclo-(dehydroAla-l-Val) (3), cyclo-(dehydroAla-l-Ile) (4), cyclo-(dehydroAla-l-Leu) (5), cyclo-(dehydroAla-l-Phe) (6), cyclo-(l-Val-l-Phe) (7), cyclo-(l-Leu-l-Phe) (8), cyclo-(l-Trp-l-Ile) (9) and cyclo-(l-Trp-l-Phe) (10). The structures of these compounds were established by extensive spectroscopic investigation, as well as by detailed comparison with literature data. This is the first report to discover pyridine, pyrimidine and diketopiperazines from the genus of Pseudogymnoascus.

Changes in grassland soil types lead to different characteristics of bacterial and fungal communities in Northwest Liaoning, China

“…The reason may be the degradation of the sampled grassland soils. Pseudogymnoascus belongs to cold-tolerant fungi, which are more suitable for survival in alpine environments ( Shi et al, 2021 ), which explains why he relative abundance of Pseudogymnoascus was the highest in temperate steppe-sandy soil.…”

“…The reason may be the degradation of the sampled grassland soils. Pseudogymnoascus belongs to cold-tolerant fungi, which are more suitable for survival in alpine environments (Shi et al, 2021), which explains why he relative abundance of Pseudogymnoascus was the highest in temperate steppe-sandy soil. Meanwhile, the more connections in the soil microbial community network, the higher its stability and the stronger the ability to inhibit the invasion of pathogens (Shi et al, 2016;Wei et al, 2018).…”

Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus

“…Fungi from the genus Pseudogymnoascus exhibit interesting capabilities in their specialized metabolism [15][16][17][18][19][20][21]. To gain a deeper understanding of Pseudogymnoascus' metabolic potential, molecular tools enabling its genetic manipulation are essential.…”

“…From a biotechnological perspective, numerous studies have demonstrated that extracts derived from diverse strains of Pseudogymnoascus harbor bioactive metabolites with antibacterial, antifungal, trypanocidal, herbicidal, and antitumoral properties [9][10][11][12][13][14]. Furthermore, chemical analyses of Pseudogymnoascus strains have revealed that members of this fungal genus produce different classes of compounds, such as sesquiterpenes [15], macrolides [16], polyketides [17,18], tetrapeptides [19], and various nitrogenous compounds [20,21]. This underscores the potential of Pseudogymnoascus as a prolific source of specialized metabolites.…”