Nitrogen removal performance and functional genes distribution patterns in solid-phase denitrification sub-surface constructed wetland with micro aeration

Sun, Haimeng; Yang, Zhongchen; Wei, Caijie; Wu, Wei‐Zhong

doi:10.1016/j.biortech.2018.04.078

Cited by 46 publications

(16 citation statements)

References 42 publications

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“…Results also exhibited the slight increase of TN concentration in T2 and T3 on the seventh day. It might be due to the shortage of carbon source required by microbes which inhibited denitrification during the continuous purification of aquaculture wastewater [31]. However, the maximum removal rates of NH 4 + -N and TN in T2 treatment were improved compared with that in T1 and T3 treatments,…”

Section: Effects Of Microbial Nanospheres On Nh 4 + -N and Tn Removalsmentioning

confidence: 98%

Purification Effect of the Aquaculture Wastewater and Sediment by Microbial Nanospheres with Different Material Ratios and Dosing Methods

et al. 2020

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Nanospheres were prepared by different materials of nano-bamboo charcoal powder, zeolite powder, and aquaculture pond sediment in different ratios. It was then fermented with effective microorganisms (EM) active calcium liquid to synthesize the bioactive microbial nanospheres. These nanospheres were used to compare the purification effect of ammonium nitrogen (NH 4 + -N), total nitrogen (TN), and total phosphorus (TP) pollutants in aquaculture wastewater. The indoor simulation experiment was also conducted to compare the different dosage methods (one-time dosing without aeration, multiple dosing without aeration, and multiple dosing with aeration) of microbial nanospheres on the removal of organic matter (OM) and effect of the biodegradability (G value) in aquaculture sediment. The results obtained indicated that the purification effect was most remarkable when the mass ratio of nano-bamboo charcoal powder: zeolite powder: pond sediment was 10%: 15%: 75%, in which the maximum removal rate of NH 4 + -N, TN, and TP reached up to 84.86%, 52.15%, and 50.35%, respectively. Under the same microbial nanospheres amount, the effect of one-time addition on the removing of OM in sediment was not as effective as that of multiple dosing. After the 20th day, the removal rate of OM reached 25.99% in multiple dosing treatment and it was 35.58% higher than one-time dosing treatment. The OM content in sediment was reduced by 32.38% under the multiple dosing with aeration treatment. Multiple dosing of microbial nanospheres with aeration increased the G value of sediment about 337.0%. In situ experiment further indicated that the microbial nanospheres dosage with aeration had a good sediment bio-remediation effect, which is applicable to solve the problem of endogenous pollution in aquaculture ponds.leads to the ecological degradation and the serious diseases [3]. Therefore, repairing the eutrophic aquaculture environment requires not only the reduction of nitrogen, phosphorus, and organic matter in the aquaculture water, but also the removal of the sediment mud. In the aspect of aquaculture water purification, the leading technologies include biological filter treatment [4], artificial floating bed cultivation [5], artificial wetland [6], and so on. Due to the shortage of freshwater resources, the high density of breeding and a large amount of bait in the aquaculture industry as well as the present aquaculture water treatments still have problems, such as single treatment method adoption, high cost, and difficult operation [7]. In recent years, a new type of circulating water pond model is applied to reduce the nitrogen and phosphorus content in the aquaculture water, mainly relying on aquatic plants and microbes function [8]. Many studies [9][10][11] have found the composite microbial agents composed of various microbes could make the control effects of aquaculture water quality better. The EM population (containing more than 80 kinds of microbes, with photosynthetic bacteria, lactic acid bacteria, yeast, and actinomycetes as repr...

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Section: Effects Of Microbial Nanospheres On Nh 4 + -N and Tn Removalsmentioning

confidence: 98%

Purification Effect of the Aquaculture Wastewater and Sediment by Microbial Nanospheres with Different Material Ratios and Dosing Methods

et al. 2020

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“…The drawback of the biodegradable polymer-based denitrification is the relative high cost in media when compared with liquid carbon [4]. On the other hand, residual DOC (dissolved organic carbon) in effluent commonly appeared in solid-phase denitrifying reactors [3,7,8], which indicated that the entirety of the carbon source was not optimally used since part of degrading bacteria have an incapable ability in denitrification [9]. Moreover, the residual organic substances were considered to support DNRA (dissimilatory nitrate reduction to ammonium) over denitrification [10], as well as the SRB (sulfate reduced to sulfide) process [3], especially in marine environment conditions.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17

et al. 2020

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An aerobic denitrification strain, Pseudomonas balearica RAD-17, was identified and showed efficient inorganic nitrogen removal ability. The average NO3−-N, NO2−-N, and total ammonium nitrogen (TAN) removal rate (>95% removal efficiency) in a batch test was 6.22 mg/(L∙h), 6.30 mg/(L∙h), and 1.56 mg/(L∙h), respectively. Meanwhile, optimal incubate conditions were obtained through single factor experiments. For nitrogen removal pathways, the transcriptional results proved that respiratory nitrate reductases encoded by napA, which was primarily performed in aerobic denitrification and cell assimilation, were conducted by gluS and gluD genes for ammonium metabolism. In addition, adding the strain RAD-17 into actual wastewater showed obvious higher denitrification performance than in the no inoculum group (84.22% vs. 22.54%), and the maximum cell abundance achieved 28.5 ± 4.5% in a ratio of total cell numbers. Overall, the efficient nitrogen removal performance plus strong environmental fitness makes the strain RAD-17 a potential alternative for RAS (recirculating aquaculture system) effluent treatment.

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“…Restoring dissolved oxygen sufficiently can limit nitrogen release, as ammonia, from sediments but can also lead to the release of more ammoniacal and total nitrogen. The aeration technology is crucial, as, for instance, micro-nano aeration results in a higher total volume and oxygen mass transfer coefficient than blast microporous aeration (Sun et al 2018). Aeration depth is also important in relation to total nitrogen release and the type of nitrogen species released, as river sediment studies have shown .…”

Section: Introductionmentioning

confidence: 99%

“…Aeration technology can also be combined with other control measures; for example, technology stimulating biofilm formation (Clifford et al 2010;Ding et al 2010;Chen et al 2013) or bioremediation (Chang et al 2013;Sun et al 2018). Moreover, it was found that the unique micro-environment formed by the root systems of floating water plants can be an important factor responsible for enhanced aeration and denitrification (Sun et al 2018). The progress in microbial remediation combined with aeration technology contrasts with the lack of effort to couple this to sediment coverage methodologies.…”

Section: Introductionmentioning

confidence: 99%

Combining artificial aeration and biological zeolite mulch for nitrogen removal from eutrophic water bodies

Wen

Wang

et al. 2020

Water Practice and Technology

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Endogenous nitrogen pollution can be a major cause of eutrophication. Nitrogen species release from sediments can be reduced by biologically-enforced zeolite mulch or water column aeration. This study was about their combined effects. Water and surface sediment samples from the Yangzhou ancient canal were aerated and biozeolite mulching was applied separately and in combination for 81 days, while the nitrogen species removal rate was recorded. The combination of aeration and biozeolite mulching removed >95% of the ammoniacal-nitrogen in 15 days. This was better than either the blank control or biozeolite mulch without aeration. The ammoniacal-nitrogen concentration was lowered faster by combined treatment than by aeration alone. Nitrate nitrogen was only detected during aeration between days 10 and18, and reached lower concentration in the presence of biozeolite. Nitrate was formed during aeration but its concentrations were higher and more variable in the absence of biozeolite; that is, mulching stabilized nitrate formation. The total nitrogen concentration reached its lowest levels after 81 days with biozeolite treatment alone, with 78% total nitrogen removal, whereas combined aeration with biozeolite achieved 41%. This shows that biozeolite mulching can remove nitrogen in eutrophic waters, even without aeration.

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Nitrogen removal performance and functional genes distribution patterns in solid-phase denitrification sub-surface constructed wetland with micro aeration

Cited by 46 publications

References 42 publications

Purification Effect of the Aquaculture Wastewater and Sediment by Microbial Nanospheres with Different Material Ratios and Dosing Methods

Purification Effect of the Aquaculture Wastewater and Sediment by Microbial Nanospheres with Different Material Ratios and Dosing Methods

Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17

Combining artificial aeration and biological zeolite mulch for nitrogen removal from eutrophic water bodies

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