Nitrogen removal in recirculating aquaculture water with high dissolved oxygen conditions using the simultaneous partial nitrification, anammox and denitrification system

Abstract

The efficient removal of nitrogen pollutants in the aquaculture systems is still a challenge due to the low concentration of organic carbon and high concentration of dissolved oxygen (DO) in the wastewater. The simultaneous partial nitrification, anammox and denitrification (SNAD) bioreactor was firstly used for the treatment of aquaculture wastewater in recirculating aquaculture system. The bioreactor operated for 180 days without adding extra organic carbon. After 60-day operation, the bioreactor reached the stable stage with the average concentration of ammonia/nitrate/nitrite/COD in the effluent with 0.26/0.75/0.47/0.27 mg/L. The Pseudoxanthomonas was the dominant genus in the biofilm samples. The typical nitrogen functional bacteria and genes for nitrification, anammox and denitrification were detected with different abundance in different procedures along the bioreactor. Network analysis revealed the significant correlations between nitrogen functional bacteria and genes. The SNAD bioreactor achieved the effective removal for nitrogen and COD under high DO conditions in recirculating aquaculture system.

Nitrogen Removal Using a Membrane Bioreactor with Rubber Particles as the Fouling Reducer

“…Among these, the contributions of Dysgonomonas, Acidobacteria, and Pantoea sp. to the nitrification process have already been reported in previous studies [22][23][24]. Lactobacillus, Erythrobacter, and Mycobacterium are known to be microflora associated with the denitrification process used in wastewater treatment plants [25][26][27].…”

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

“…Through metaproteomic approach, detected the production of denitrifying protein such as nosZ during effective nitrification of ammonia-oxidizing consortia and nitrate was not found to get accumulated in the system thereby ensuring effective denitrification (Kumar et al 2011;Jose et al 2020). Very recently, simultaneous partial nitrification, anammox and denitrification (SNAD) bioreactor was successfully demonstrated for the first time in RAS with high dissolved oxygen conditions and without any additional organic carbon supply (Lu et al 2020). Here, a significant correlation was observed between the nitrogen functional bacteria and their corresponding genes through network analysis.…”

A Review of Ammonia-Oxidizing Archaea and Anaerobic Ammonia-Oxidizing Bacteria in the Aquaculture Pond Environment in China

“…The AnAOB activity could be inhibited by the presence of oxygen ( Liu et al, 2016 ); so far, no anammox activity has been reported in pond aquaculture water. However, AnAOB can be detected in aerated systems, such as nitrogen-removing moving bed biofilters ( van Kessel et al, 2010 ; Persson et al, 2014 ), rotating biological contactor and biofilters ( Egli et al, 2003 ), and the simultaneous partial nitrification, anammox, and denitrification (SNAD) bioreactor ( Lu J. et al, 2020 ). They probably inhabit the oxygen-depleted zones of aerated biofilters ( Mulder et al, 1995 ), where an anoxic environment is created by AOA, AOB, and heterotrophic bacteria.…”

“…Research has shown a bioreactor start-up for 180 days without adding extra organic carbon. After a 60-day operation, the bioreactor reached a stable stage with the average concentration of ammonia/nitrate/nitrite/COD in the effluent of 0.26/0.75/0.47/0.27 mg L –1 ( Lu J. et al, 2020 ). Because they do not need extra organic carbon and are energy-saving, SNAD bioreactors have good prospects.…”

“…The bioreactors can be moving bed biofilters, SNAD bioreactors, etc. ( Lu J. et al, 2020 ). This bioreactor can greatly reduce N 2 O emissions during pond aquaculture because the nitrogen removal process is mainly carried out by AOA and AnAOB.…”