Nitrogen isotope analysis of free glycine in soil using isotope dilution mass spectrometry

Norlin, Elin; Irgum, Knut; Ohlsson, K. E. Anders

doi:10.1016/j.soilbio.2006.09.001

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…The resultant derivatives vary in molecular size, separation efficiency, and combustion efficiency . Similarly, abundant combinations are found among other derivatization reagents, such as the alkyl chloroformates . As with all syntheses, temperature, reaction time, reagent and analyte concentration, and other critical variables, also affect the outcome of derivatization.…”

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confidence: 99%

“…[9,15] Similarly, abundant combinations are found among other derivatization reagents, such as the alkyl chloroformates. [11,[16][17][18][19][20] As with all syntheses, temperature, reaction time, reagent and analyte concentration, and other critical variables, also affect the outcome of derivatization. While the quality of measurement varies among amino acids both within and between derivatization techniques, the mean repeatability is generally reported to be ≤1‰ for δ 15 N values.…”

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confidence: 99%

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The relative influence of derivatization and normalization procedures on the compound‐specific stable isotope analysis of nitrogen in amino acids

Yarnes

Herszage

2017

Rapid Comm Mass Spectrometry

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The overall accuracy and precision of δ N-AA measurements were improved following both compound-specific calibration and scale-normalization, as was the comparability of δ N-AA measurements of individual amino acids between derivatization techniques across organisms. The mean difference of scale-normalized δ N-AA values across all organisms between the two derivatization techniques was 0.19‰, much less than the typical analytical error associated with δ N-AA measurements (±1‰) CONCLUSIONS: Adoption of standardized calibration procedures will be important to establishing reproducibility in δ N-AA measurements, particularly across derivatization techniques. It is both technically practical and desirable for users of CSIA-AA to adopt practices in quality control and assessment similar to those outlined for BSIA, including the compound-specific calibration of δ N-AA values, followed by scale-normalization. Copyright © 2017 John Wiley & Sons, Ltd.

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confidence: 99%

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confidence: 99%

The relative influence of derivatization and normalization procedures on the compound‐specific stable isotope analysis of nitrogen in amino acids

Yarnes

Herszage

2017

Rapid Comm Mass Spectrometry

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“…Isotope fractionations during GC‐C‐IRMS measurements have been reported for carbon and nitrogen at natural abundance when varying gas chromatographic temperature gradients . Major differences in δ 15 N‐values of standard amino acids treated by cation‐exchange chromatography from those of untreated samples were not observed at natural abundance . However, an isotope shift of δ 15 N‐values of different amino acids of approximately −2‰ has been observed by others .…”

Section: Resultsmentioning

confidence: 82%

“…Moreover, a dependence of measured isotope ratios on the GC oven temperature gradient has been observed for GC‐C‐IRMS measurements of nitrogen and carbon . However, memory effects are rarely reported as potential sources of error, particularly when analysing compounds enriched in a specific isotope using continuous flow isotope ratio measurement . Significant memory effects can occur in the GC‐C‐IRMS measurement of 13 C/ 12 C isotope ratios, for example with fatty acid methyl esters, when mixtures of 13 C‐enriched (500‰ δ 13 C) and unlabelled fatty acids co‐elute …”

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confidence: 99%

Practical recommendations for the reduction of memory effects in compound‐specific ¹⁵N/¹⁴N‐ratio analysis of enriched amino acids by gas chromatography/combustion/isotope ratio mass spectrometry

Petzke

Metges

2011

Rapid Comm Mass Spectrometry

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Gas chromatography/combustion/isotope ratio mass spectrometry (GC-C-IRMS) is a highly sensitive approach which allows the analysis of the (13)C/(12)C and (15)N/(14)N isotope composition of amino acids in the range of natural abundance or in slightly (13)C- and (15)N-enriched samples. However, the accuracy of measurements remains a permanent challenge. Here we show the effect of the presence of slightly (15)N-enriched compounds in physiological samples on the accuracy and reproducibility of (15)N-abundances of amino acids within or between analytical runs. We spiked several individual amino acids with the respective (15)N-labelled isotopomer and measured the (15)N/(14)N ratios of other amino acids in the same sample or in the following analytical runs. Intra- and inter-run memory effects can be observed in (15)N/(14)N ratios of amino acids. Sample throughput is reduced when cleaning runs using standard mixtures are required to restore initial conditions after runs of samples with (15)N-enriched analytes. Possible reasons for the observed phenomenon and its implications for work in the lower (15)N-enrichment range (<0.5 APE) are discussed and include different aspects of gas chromatography, derivatisation, and hot catalytic metal surface effects. Results need to be interpreted with caution if complex physiological samples contain (15)N-enriched amino acids beyond 500‰ δ(15)N (~0.18 APE).

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“…In addition, glycine is one of the most abundant free amino acids in soil and can be utilized rapidly by microorganisms [35]. The concentration of 13 C, 15 N-glycine in soil solution and the microbial biomass was measured using a compound-specific stable isotope analysis procedure described by Geisseler and Horwath (2014) [5].…”

Section: Methodsmentioning

confidence: 99%

Nitrogen Fertilizer and Straw Applications Affect Uptake of 13C,15N-Glycine by Soil Microorganisms in Wheat Growth Stages

Yang

Zhang

et al. 2017

PLoS ONE

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This study investigated the influence of nitrogen (N) fertilizer and straw on intact amino acid N uptake by soil microorganisms and the relationship between amino acid turnover and soil properties during the wheat growing season. A wheat pot experiment was carried out with three treatments: control (CK), N fertilizer (NF) and N fertilizer plus rice straw (NS). We used stable isotope compound-specific analysis to determine the uptake of 13C,15N-glycine by soil microorganisms. In the NF treatment, microbial 13C,15N-glycine uptake was lower compared with CK, suggesting that inorganic N was the preferred N source for soil microorganisms. However, The application of straw with N fertilizer (in NS treatment) increased microbial 13C,15N-glycine uptake even with the same amount of N fertilizer application. In this treatment, enzyme activities, soil microbial biomass C and microbial biomass N increased simultaneously because more C was available. Soil mineral N and plant N contents all decreased substantially. The increased uptake of intact 13C,15N-glycine in the NS treatment can be attributed to direct assimilation by soil microorganisms to satisfy the demand for N when inorganic N was consumed.

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Nitrogen isotope analysis of free glycine in soil using isotope dilution mass spectrometry

Cited by 8 publications

References 37 publications

The relative influence of derivatization and normalization procedures on the compound‐specific stable isotope analysis of nitrogen in amino acids

The relative influence of derivatization and normalization procedures on the compound‐specific stable isotope analysis of nitrogen in amino acids

Practical recommendations for the reduction of memory effects in compound‐specific ¹⁵N/¹⁴N‐ratio analysis of enriched amino acids by gas chromatography/combustion/isotope ratio mass spectrometry

Nitrogen Fertilizer and Straw Applications Affect Uptake of 13C,15N-Glycine by Soil Microorganisms in Wheat Growth Stages

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Nitrogen isotope analysis of free glycine in soil using isotope dilution mass spectrometry

Cited by 8 publications

References 37 publications

The relative influence of derivatization and normalization procedures on the compound‐specific stable isotope analysis of nitrogen in amino acids

The relative influence of derivatization and normalization procedures on the compound‐specific stable isotope analysis of nitrogen in amino acids

Practical recommendations for the reduction of memory effects in compound‐specific 15N/14N‐ratio analysis of enriched amino acids by gas chromatography/combustion/isotope ratio mass spectrometry

Nitrogen Fertilizer and Straw Applications Affect Uptake of 13C,15N-Glycine by Soil Microorganisms in Wheat Growth Stages

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Practical recommendations for the reduction of memory effects in compound‐specific ¹⁵N/¹⁴N‐ratio analysis of enriched amino acids by gas chromatography/combustion/isotope ratio mass spectrometry