Nitrogen influence on suspended vs biofilm growth and resource recovery potential of purple non-sulfur bacteria treating fuel synthesis wastewater

Shaikh, S.; Rashid, Naim; McKay, Gordon; Liberski, A.R.; Mackey, Hamish R.

doi:10.1016/j.bej.2022.108754

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…Even in the absence of nitrogen, a protein content as high as 43 % was achieved under nitrogen‐limited conditions. A comparable biomass protein content was obtained in a similar nitrogen‐deficient study 14. Usually, nitrogen‐deficient conditions are considered for biopolymer and hydrogen production 24, 25.…”

Section: Resultssupporting

confidence: 59%

“…The reduced removal efficiency was most probably due to the absence of nitrogen. In one study that cultured PNSB under nitrogen-deficient and sufficient conditions, cells in nitrogen-sufficient conditions had a higher organic removal efficiency and achieved peak exponential growth within a week, while cells in nitrogen-sufficient conditions had a slower removal efficiency and achieved peak exponential growth over two weeks [14]. Furthermore, among the different pilot conditions, R2-20.1 had the highest removal rates (2.1 ± 0.13 mg TOC L -1 h -1 ), while R1-26.4 and R2-13.8 trial groups had removal rates around 1.3 mg TOC L -1 h -1 .…”

Section: Resultsmentioning

confidence: 99%

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Converting Fuel‐Synthesis Process Water to Aquaculture Feed by Purple Non‐Sulfur Bacteria

et al. 2023

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The availability of agricultural wastewater in arid climes limits the possibility of exploiting purple non‐sulfur bacteria (PNSB) to recover aqua‐feed bioproducts. Thus, this study examines the feasibility of recovering such bioproducts using fuel‐synthesis process water (FSPW) as a feedstock. Experiments were performed under varying light conditions. The results revealed that PNSB cultured in the zero‐nitrogen feedstock had over 40 % protein content, contained substantial lipids and pigments, and reduced FSPW organics by over 70 %. The light intensity significantly impacted biomass constituents and treatment efficiency. The results are promising for the potential development of a circular economy to convert FSPW to aqua‐feed.

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Section: Resultssupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Converting Fuel‐Synthesis Process Water to Aquaculture Feed by Purple Non‐Sulfur Bacteria

et al. 2023

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“…It is suspected that the lower EPS production of PNSB [129] may limit adherence to the carriers, although the same cultures have successfully adhered to other polymeric materials [154]. Woven-polymer shade cloth has been the most successful of the materials trialed for biofilm formation, which may have potential as carrier materials [155]. There remains a significant gap in the area of carrier design and adhesion for PNSB to successfully implement an MBBPR.…”

Section: Moving-bed Biofilm Photobioreactor (Mbbpr)mentioning

confidence: 99%

Photobioreactor Design for Polyhydroxyalkanoate Production Using Anoxygenic Photoheterotrophs: A Review

Shaikh,

Rashid,

McKay

et al. 2023

Fermentation

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This review paper provides an overview of various types of photobioreactors (PBRs) that could be used for the production of polyhydroxyalkanoates (PHAs) using anoxygenic photoheterotrophs, with a focus on the design and operation of these systems. The paper highlights the potential of different PBRs based on reactor geometry and growth mode, and also examines the advantages and disadvantages of each PBR type and summarizes their suitability for PNSB-PHA production. The optimization of reactor design and operation is crucial for maximizing PNSB growth and PHA productivity. The self-immobilization of bacteria in granular sludge is a promising technology for wastewater treatment and the production of PHAs, while grooved-surface PBRs and porous-substrate PBRs have limitations due to difficult biomass harvesting in the former and the presence of aerobic conditions incongruent with PNSB culturing in the latter. Limitations exist with all solutions for maximizing rapid growth and maintaining high biomass concentrations due to the requirements of phototrophic growth.

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Valorization of purple non-sulfur bacteria biomass from anaerobic treatment of fuel synthesis process wastewater to microbial protein: a means of enhancing food security in arid climates

Wada

Onwusogh

Vincent

et al. 2023

Biomass Conv. Bioref.

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The global shift from traditional fish farming to aquaculture has created an aquafeed production gap. Hence, the recovery of microbial protein from organic and nutrient-rich agro-industrial wastewaters has been identified as a suitable substitute. However, such waste streams are sparse in arid climes like the Middle East. Thus, this study explores the potential of single-cell protein recovery from a novel waste stream abundant in the region–fuel synthesis process water (FSPW), via anaerobic treatment with purple non-sulfur bacteria (PNSB). The feedstock (COD = 10.3 g/L) amended with essential nutrients was inoculated with a PNSB-dominated mixed culture in replicate 1-L batch fermenters. The wastewater characteristics and microbial biomass assays were performed using standard methods. Around two-thirds of the COD was degraded within 72 h at a rate of 2100 mg L−1d−1, which reduced to about 710 mg L−1d−1 by trial end. Also, total nitrogen levels (90 mg/L) were depleted within 72 h, indicating that nitrogen was a limiting nutrient. In addition, a peak biomass concentration of 1.11±0.037 gvss/L was obtained. Proximate analysis revealed that the biomass consisted of 35% protein, 32% lipid, 16% carbohydrate, 7% ash, 0.5% carotenoids, 0.6% bacteriochlorophylls, and 0.004% coenzyme Q10. Biomass protein’s amino acid profile was comparable to soybean grain and meets dietary requirements for several aquatic livestock. Metal analysis of the biomass and wastewater indicated that nutritionally undesirable metals were undetected. Results show that PNSB not only efficiently degrade FSPW’s organic load but also upcycles the waste to valuable feed constituents, potentially creating a regional circular economy.

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Nitrogen influence on suspended vs biofilm growth and resource recovery potential of purple non-sulfur bacteria treating fuel synthesis wastewater

Cited by 7 publications

References 43 publications

Converting Fuel‐Synthesis Process Water to Aquaculture Feed by Purple Non‐Sulfur Bacteria

Converting Fuel‐Synthesis Process Water to Aquaculture Feed by Purple Non‐Sulfur Bacteria

Photobioreactor Design for Polyhydroxyalkanoate Production Using Anoxygenic Photoheterotrophs: A Review

Valorization of purple non-sulfur bacteria biomass from anaerobic treatment of fuel synthesis process wastewater to microbial protein: a means of enhancing food security in arid climates

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