Nitrogen fixation by legumes in the tropics

Gibson, A. H.; Dreyfus, B; Dommergues, Yvon

doi:10.1007/978-94-009-7529-3_2

Cited by 35 publications

(14 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The rhizobial (MPN) counts obtained on siratro were consistently high in all the soils apart from the Onne soil. These results agree with the suggestion that siratro is a promiscuous host (Gibson et al . 1982) although the spectrum of rhizobia nodulating this host appears to differ from that nodulating C. calothyrsus , L. leucocephala and G. sepium (Bala & Giller 2001).…”

Section: Discussionsupporting

confidence: 93%

“…Population estimates of indigenous rhizobia in each soil differed depending on the trap host (Table 3). Since such variations were expected, siratro ( Macroptilium atropurpureum ) was included in the assay as a ‘universal host’ because of its ability to nodulate with a broad range of rhizobia including fast‐ and slow‐growing types (Gibson et al . 1982).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Distribution and diversity of rhizobia nodulating agroforestry legumes in soils from three continents in the tropics

2003

View full text Add to dashboard Cite

The natural rhizobial populations of Calliandra calothyrsus, Gliricidia sepium, Leucaena leucocephala and Sesbania sesban were assessed in soils from nine sites across tropical areas of three continents. The rhizobial population size varied from undetectable numbers to 1.8 x 104 cells/g of soil depending on the trap host and the soil. Calliandra calothyrsus was the most promiscuous legume, nodulating in eight soils, while S. sesban nodulated in only one of the soils. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analyses of the 16S rRNA gene and the internally transcribed spacer (ITS) region between the 16S and 23S rRNA genes were used to assess the diversity and relative abundance of rhizobia trapped from seven of the soils by C. calothyrsus, G. sepium and L. leucocephala. Representatives of the 16S rRNA RFLP groups were also subjected to sequence analysis of the first 950 base pairs of the 16S rRNA gene. Eighty ITS groups were obtained, with none of the ITS types being sampled in more than one soil. RFLP analysis of the 16S rRNA yielded 23 'species' groups distributed among the Rhizobium, Mesorhizobium, Sinorhizobium and Agrobacterium branches of the rhizobial phylogenetic tree. The phylogeny of the isolates was independent of the site or host of isolation, with different rhizobial groups associated with each host across the soils from widely separated geographical regions. Although rhizobial populations in soils sampled from the centre of diversity of the host legumes were the most genetically diverse, soil acidity was highly correlated with the diversity of ITS types. Our results support the hypothesis that the success of these tree legumes in soils throughout the tropics is the result of their relative promiscuity (permissiveness) allowing nodulation with diverse indigenous rhizobial types.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Distribution and diversity of rhizobia nodulating agroforestry legumes in soils from three continents in the tropics

2003

View full text Add to dashboard Cite

show abstract

“…Outro fator pelo qual se esperaria encontrar mais isolados de crescimento lento na floresta é a utilização de siratro como planta-isca. O siratro é considerado uma espécie nodulada, principalmente, por estirpes do gênero Bradyrhizobium (Gibson et al, 1982;Thies et al, 1991;Odee et al, 1997). Porém, neste e em outros estudos (Pereira, 2000), não se verificou seletividade do siratro para estirpes de crescimento lento uma vez que uma proporção significativa de estirpes de crescimento rápi-do nodularam esta espécie.…”

Section: Resultsunclassified

Diversidade de bactérias que nodulam siratro em três sistemas de uso da terra da Amazônia Ocidental

Jesus

Moreira

Florentino

et al. 2005

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi verificar o efeito de três sistemas de uso da terra da Amazônia Ocidental sobre a diversidade fenotípica de bactérias que nodulam siratro. Os sistemas estudados foram cultivo de mandioca, cultivo de pupunheira e floresta de terra firme. As densidades de bactérias foram avaliadas pela técnica do número mais provável, pela inoculação de suspensões de solo diluídas em siratro (Macroptilium atropurpureum), e foram semelhantes nas áreas estudadas. Foram caracterizados 257 isolados, provenientes dos nódulos de siratro, os quais formaram 63 grupos culturais a 80% de similaridade. Poucos isolados foram obtidos a partir de solo sob floresta, enquanto um maior número de isolados foi obtido nas áreas cultivadas. A diversidade pelo índice de Shannon e a riqueza foram maiores na área com mandioca e menores na floresta. Porém, a riqueza foi similar nos três sistemas quando determinada pela análise de rarefação. Houve diferença na proporção de tipos de culturais, a qual pôde ser explicada pela diferença na utilização da terra. Houve influência dos sistemas de uso da terra sobre a diversidade de bactérias que nodulam leguminosas.Termos para indexação: rizóbio, Macroptilium atropurpureum, densidade, caracterização cultural. Leguminosae nodulating bacteria diversity from three different land use systems in Brazilian Western AmazonAbstract -The aim of this work was to evaluate the effect of three different land use systems from the Brazilian Western Amazon on Leguminosae nodulating bacteria diversity. The land use systems studied were cassava crop, peach palm crop and upland forest. Rhizobial densities were evaluated by the most probable number technique, after inoculation of Macroptilium atropurpureum with diluted soil samples, and they were similar for the three systems studied. Two hundred and fifty-seven bacteria isolated from the nodules were characterized and clustered into 63 groups of isolates with 80% similarity. Few isolates were obtained from forest, whereas a large number of isolates was obtained in the crop systems. The highest richness and Shannon diversity were found in the cassava crop and the lowest ones were found in the forest. However, the richness was similar for the three systems when it was determined with a rarefaction analysis. Differences in the proportion of types of growth were found and can be explained by differences amongst the systems. The land use systems influenced the Leguminosae nodulating bacterial diversity.

show abstract

“…The low aluminium content in fallow could be due to the residual effect of the wood ash from the slash-and burn practice. Agoumé and Birang [20] also reported that, aluminium saturation was significantly affected by the LUSs with the lowest aluminium saturation obtained in soils of Chromolaena odorata fallows. They noted that soils of fallows presented a higher fertility level as compared to those of the secondary forests and cocoa plantations.…”

Section: Discussionmentioning

confidence: 89%

Abundance of Legume Nodulating Bacteria in Soils of Diverse Land Use Systems in Cameroon

Nkot¹,

Fankem²,

Adamou³

et al. 2015

ujps

View full text Add to dashboard Cite

Improving biological nitrogen fixation through legume nodulating bacteria (LNB) inoculation requires knowledge on the abundance and effectiveness of indigenous population in the ferralsols. The Most Probable Number method (MPN) was used to determine the naturally occurring LNB population in 64 representative soils of four sites in the humid forest zone of Cameroon. Nodulation Macroptilium atropurpureum was examined under tubes experiment in four location sites: Bertoua, Ebolowa, Bokito and Yaounde, and within each of the locations, in four land use systems (LUSs) of different levels of disturbance: mixed farm; fallows; cocoa plantation and forest. The LNB population size varied from 0.78 to 5.25 log units of soil depending on the land use. The most disturbed LUSs (farms and fallows) exhibiting the highest number of LNB on average. Undisturbed ones (cocoa plantation and forest) showed the lowest number of LNB. The percentage of the need for inoculation was more frequent in the forests (94%) and plantations (75 %). It is low in the in the fallows (25%) and the farms (44 %). In total 60 % of the soils analysed need inoculation. These results are important for future investigations on alternative inoculant strains for improving legume production in Cameroon.

show abstract

Nitrogen fixation by legumes in the tropics

Cited by 35 publications

References 84 publications

Distribution and diversity of rhizobia nodulating agroforestry legumes in soils from three continents in the tropics

Distribution and diversity of rhizobia nodulating agroforestry legumes in soils from three continents in the tropics

Diversidade de bactérias que nodulam siratro em três sistemas de uso da terra da Amazônia Ocidental

Abundance of Legume Nodulating Bacteria in Soils of Diverse Land Use Systems in Cameroon

Contact Info

Product

Resources

About