Nitrofurazone-loaded electrospun PLLA/sericin-based dual-layer fiber mats for wound dressing applications

Abstract

The ideal wound dressing should achieve rapid healing with hemostasis, antimicrobial activity, maintenance of a moist wound bed, etc. The electrospun blend mat meets the requirements outlined for wound healing, by their microfibrous structures providing a suitable environment for wound healing. In this study, a duallayer nanofiber blend mat wound dressing of nitrofurazone (NFZ)-loaded poly(L-lactide) (PLLA)/sericin nanofibers and NFZ-loaded PLLA nanofibers is fabricated by electrospinning. The NFZ-loaded PLLA/ sericin nanofibers act as the first layer and then the NFZ-loaded PLLA nanofibers are the second layer to prepare the dual-layer fiber dressings. The prepared dual-layer NFZ-loaded fiber dressings have satisfactory antibacterial activity against both Gram-positive and Gram-negative bacteria. In vitro drug release studies show that the drug release profiles can be controlled by adjusting the drug amount in different layers of the dual-layer dressings. The methyl thiazolyl tetrazolium (MTT) assays demonstrate that the NFZ-loaded PLLA/sericin fibers are nontoxic and biocompatible. In vivo wound healing tests are performed in rats. The results reveal that the dual-layer fiber dressings perform better than commercial non-woven dressing in decreasing wound size. It is observed at 12 days that the wound size reduction is 97% for being dressed with the dual-layer fiber dressings but 84% for the commercial woven dressing.These NFZ-loaded PLLA/sericinkPLLA dual-layer fiber mats may provide a promising candidate for accelerating wound healing.

Poly (lactic acid)-based biomaterials for orthopaedic regenerative engineering

“…In addition, due to their small fiber diameters, they mimic the nano-sized physical characteristics of the ECMs. Growth factors, anti-bacterial drugs, inorganic minerals, and other factors have been embedded and sequestered in the electrospun nanofibrous matrix (for example, using NPs), and delivered appropriately [104, 169, 170]. However, these scaffolds do not possess the appropriate pore size (>200 μm) for osteoblasts/MSCs migration and proliferation.…”

Antibacterial chitosan/silk sericin 3D porous scaffolds as a wound dressing material

“…Zhao et al fabricated a dual-layer fiber dressing that included nitrofurazone (NFZ)-loaded poly(L-lactide) (PLLA)/ sericin nanofibers as the first layer and NFZ-loaded PLLA nanofibers by electrospinning. The performance of these fiber-dressings as an ideal wound dressing were investigated and this research reported that different compositions of dual-layer fiber mats had porosities between 75.14 ± 5.43% and 78.35 ± 2.38% (Zhao et al 2015). The obtained porosity percentage is high enough for usage as a wound-dressing material.…”

Poly(L-lactic acid) Modified by Magnesium Phenylmalonate: Thermal Behavior, Processing Fluidity, and Mechanical Properties

“…Poly(L-lactic acid) (PLLA), as one of the most important biodegradable polymers, has achieved the large-scale production through a lot of sustained hard work of multinationals such as Nature Works LLC, Mitsui Chemicals Group, etc. And PLLA also represents clearly an alternative to petroleum-based polymers in food packaging [1,2], disposable tableware [3], and mulch films [4] because of its biodegradability, nontoxic, good biocompatibility [5,6]. For example, nisinloaded chitosan/PLLA antimicrobial films were fabricated via coating method, and the results from diffusion behavior and antimicrobial activity of nisin-loaded chitosan/PLLA films revealed well-controlled release and antimicrobial activity against Staphylococcus aureus ATCC6538, showing that nisin-loaded chitosan/PLLA films may be a promising active food packaging film [7].…”