Nitrile hydratase activity of Nocardia corallina B-276

Abstract

A atividade da nitrila hidratase de Nocardia corallina B-276 é descrita. O único produto detectado, na hidrólise biocatalisada das nitrilas selecionadas, foi a amida correspondente. O melhor resultado foi obtido usando-se suspensão de células em tampão de fosfatos (pH = 7,0), com porcentagens de conversão na faixa de 31% a > 99%.The nitrile hydratase activity of Nocardia corallina B-276 is described, and the only detected product during the biocatalyzed hydrolysis of selected nitriles was the corresponding amide. The best results were obtained using a suspension of cells in a phosphate buffer (pH= 7.0), with conversion percentages ranging from 31% to > 99%.

Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276

“…The cells of N. corallina B-276 (ATCC 31338) were grown following the method reported by Pérez et al . [5]. The biocatalysed hydrolysis of 1 was carried out according to our previously reported conditions [5,16,17]:…”

“…Method B : The cells were harvested [5] and suspended in a phosphate buffer solution (0.1 M, pH 7.0). Two substrate:dry cells ratios were used in the biotransformation: 1:5 and 1:17 (w/w).…”

“…Recent studies have demonstrated enantioselective biotransformation of nitriles using nitrile-hydrolysing microorganisms [3,4]; we have also previously reported the ability of Nocardia corallina B-276 (a Gram-positive actinomycete) to hydrolyse achiral nitriles to amides [5]. It is noteworthy that the laboratory of Ohta, in 1991, was the first group to report the enzymatic hydrolysis of 2-substituted nitriles to give optically active 2-arylalkanoic acids [6].…”

Application of the redox system of Nocardia corallina B-276 in the enantioselective biotransformation of ketones and alcohols

“…) and solution C (glucose 2.00 g L -1 , J. T. Baker, USA); the pH was adjusted to 8.50±0.5. The culture was incubated in an orbital shaker at 28-30 °C, 150 rpm for 96 h. 19 The cells were collected by centrifugation at 4500 rpm for 15 min, and the pH of the supernatant was measured. The cells were washed twice with potassium phosphate buffer (0.1 mol L -1 , pH 7.00) and centrifuged at 4500 rpm for 15 min.…”

Effect of agitation and aeration on the production of nitrile hydratase by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor

“…It has been known that the nitrile hydratases from Brevibacterium sp. R312 (Thiery et al 1986), Nocardia corallina B-276 (Pérez et al 2005) and Rhodococcus sp. N774 (Endo et al 2001) contain iron atom as a cofactor.…”