Nitric oxide triggers a concentration-dependent differential modulation of superoxide dismutase (FeSOD and Cu/ZnSOD) activity in sunflower seedling roots and cotyledons as an early and long distance signaling response to NaCl stress

Arora, Dhara; Bhatla, Satish C.

doi:10.1080/15592324.2015.1071753

Cited by 33 publications

(14 citation statements)

References 52 publications

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“…The NO was found to have dual role on SOD by positively modulating the FeSOD expression and negatively affecting Cu/ZnSOD one. With this, NO furnished a differential antioxidant protection to salt-stressed sunflower (Helianthus annuus) seedlings (Arora & Bhatla 2015). Likewise, the challenge of tobacco roots with NaCl led to accumulation of endogenous NO in leaves that was accompanied by an increment of SOD and CAT activities (da-Silva et al 2017).…”

Section: Nitric Oxidementioning

confidence: 99%

“…Aegiceras corniculatum Cucumis sativus (Fan et al 2007) Cynodon dactylon (Liu et al 2016) Glycine max (Simaei et al 2012) Gossypium hirsutum Helianthus annuus (Arora & Bhatla 2015;Kaur & Bhatla 2016) Hordeum vulgare (Li et al 2008) Nicotiana tabacum (da-Silva et al 2017) Solanum lycopersicum (Manai et al 2014) Spinacia oleracea (Du et al 2015) Triticum aestivum (Zheng et al 2008;Hasanuzzaman et al 2011) Increase of K + /Na + ratio…”

Section: Activation Of Antioxidant Systemmentioning

confidence: 99%

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Hydrogen sulfide: a new endogenous player in an old mechanism of plant tolerance to high salinity

da-Silva

Modolo

2017

Acta Bot. Bras.

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High salinity aff ects plants due to stimulation of osmotic stress. Cell signaling triggered by nitric oxide (NO) and hydrogen sulfi de (H 2 S) activates a cascade of biochemical events that culminate in plant tolerance to abiotic and biotic stresses. For instance, the NO/H 2 S-stimulated biochemical events that occur in plants during response to high salinity include the control of reactive oxygen species, activation of antioxidant system, accumulation of osmoprotectants in cytosol, induction of K + uptake and Na + cell extrusion or its vacuolar compartmentation among others. Th is review is a compilation of what we have learned in the last 10 years about NO participation during cell signaling in response to high salinity as well as the role of H 2 S, a new player in the mechanism of plant tolerance to salt stress. Th e main sources of NO and H 2 S in plant cells is also discussed together with the evidence of interplay between both signaling molecules during response to stress.

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Section: Nitric Oxidementioning

confidence: 99%

Section: Activation Of Antioxidant Systemmentioning

confidence: 99%

Hydrogen sulfide: a new endogenous player in an old mechanism of plant tolerance to high salinity

da-Silva

Modolo

2017

Acta Bot. Bras.

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“…In continuation of our deeper understanding of the signaling mechanisms associated with seed germination in this important oilseed crop, over the past decade, we have further undertaken critical analysis of various salt stress tolerance mechanisms in sunflower seedlings, both as a rapid response to salt and also in long‐distance sensing of salt stress by the seedling cotyledons. In this context, we have very recently reported the crucial role of superoxide dismutase (Arora and Bhatla ), GSH reductase (Kaur and Bhatla ) and heme oxygenase (Singh and Bhatla ) in modulating salt stress tolerance by sunflower seedlings in coordination with endogenous NO. Thus, on the basis of these strong evidence available from our previous publications on NO crosstalk with the above‐stated ROS‐scavenging enzymes, our current objective is to further understand these molecular interactions at the proteome level.…”

Section: Introductionmentioning

confidence: 99%

S‐nitrosylation/denitrosylation as a regulatory mechanism of salt stress sensing in sunflower seedlings

Jain

Toerne

Lindermayr

et al. 2017

Physiologia Plantarum

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Nitric oxide (NO) and various reactive nitrogen species produced in cells in normal growth conditions, and their enhanced production under stress conditions are responsible for a variety of biochemical aberrations. The present findings demonstrate that sunflower seedling roots exhibit high sensitivity to salt stress in terms of nitrite accumulation. A significant reduction in S-nitrosoglutathione reductase (GSNOR) activity is evident in response to salt stress. Restoration of GSNOR activity with dithioerythritol shows that the enzyme is reversibly inhibited under conditions of 120 mM NaCl. Salt stress-mediated S-nitrosylation of cytosolic proteins was analyzed in roots and cotyledons using biotin-switch assay. LC-MS/MS analysis revealed opposite patterns of S-nitrosylation in seedling cotyledons and roots. Salt stress enhances S-nitrosylation of proteins in cotyledons, whereas roots exhibit denitrosylation of proteins. Highest number of proteins having undergone S-nitrosylation belonged to the category of carbohydrate metabolism followed by other metabolic proteins. Of the total 61 proteins observed to be regulated by S-nitrosylation, 17 are unique to cotyledons, 4 are unique to roots whereas 40 are common to both. Eighteen S-nitrosylated proteins are being reported for the first time in plant systems, including pectinesterase, phospholipase D-alpha and calmodulin. Further physiological analysis of glyceraldehyde-3-phosphate dehydrogenase and monodehydroascorbate reductase showed that salt stress leads to a reversible inhibition of both these enzymes in cotyledons. However, seedling roots exhibit enhanced enzyme activity under salinity stress. These observations implicate the role of S-nitrosylation and denitrosylation in NO signaling thereby regulating various enzyme activities under salinity stress in sunflower seedlings.Abbreviations -ABC, ammonium bicarbonate; ACN, acetonitrile; APX, ascorbate peroxidase; BCIP/NBT, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium; BSA, bovine serum albumin; BST, biotin-switch tech-

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“…The work has been undertaken using dark-grown sunflower seedlings which is an established system for varied investigations in the author's laboratory in the past decade. [27][28][29][30][31] After undertaking extensive investigations on NO estimation in seedlings raised over a wide concentration range of these compounds, detailed investigations on the implications of endogenous NO available in seedling roots and cotyledons have been analyzed in terms of impact of NO on peroxynitrite content, total ROS and H 2 O 2 accumulation in the tissue under specified treatments of NO donors or NO scavengers. Investigations using SNP have been undertaken both in light and dark, to resolve the controversy of its usage in these two conditions.…”

Section: Introductionmentioning

confidence: 99%

Signaling through reactive oxygen and nitrogen species is differentially modulated in sunflower seedling root and cotyledon in response to various nitric oxide donors and scavengers

Singh

Bhatla

2017

Plant Signaling & Behavior

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Sodium nitroprusside (SNP), diethylenetriamine NONOate (DETA), S-nitroso-n-acetyl-D,L- penicillamine (SNAP), and 4-(p-methoxyphenyl)-1,3,2- Oxathiazolylium-5-olate (CAY) exhibit differential NO releasing ability in aqueous solution and hemoglobin is a more efficient NO quencher than cPTIO in solution. DETA releases 16% more NO compared with SNP in solution. Various NO donors (SNP, DETA, SNAP, and CAY) also bring about a differential but concentration-dependent increase in endogenous NO in seedling cotyledons and roots. Two-day old, dark-grown seedling roots exhibit 95%, 77%, 59% and 45% increase in NO content in presence of each of 500 µM of DETA, SNAP, CAY and SNP, respectively, relative to control. NO accumulation in the tissue system as a response to NO donors is reflected in terms of corresponding peroxynitrite accumulation. Release of cyanide and free iron as byproducts of SNP dissociation in solution limits its usefulness as an NO donor. SNP leads to profuse ROS generation in sunflower seedling roots. Light is not a pre-requisite for NO generation from SNP. Present work also demonstrates the usefulness of hemoglobin over cPTIO as NO scavenger. Hemoglobin brings about increasing NO quenching with its increasing concentration from 2.5 to 10 µM. Greater sensitivity of the root system to the NO donor/scavenger treatments is evident, it being in direct contact with the molecules in the incubation/ growth medium. This differential effect does not seem to be significantly transmitted to the cotyledons (long-distance signaling).

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Nitric oxide triggers a concentration-dependent differential modulation of superoxide dismutase (FeSOD and Cu/ZnSOD) activity in sunflower seedling roots and cotyledons as an early and long distance signaling response to NaCl stress

Cited by 33 publications

References 52 publications

Hydrogen sulfide: a new endogenous player in an old mechanism of plant tolerance to high salinity

Hydrogen sulfide: a new endogenous player in an old mechanism of plant tolerance to high salinity

S‐nitrosylation/denitrosylation as a regulatory mechanism of salt stress sensing in sunflower seedlings

Signaling through reactive oxygen and nitrogen species is differentially modulated in sunflower seedling root and cotyledon in response to various nitric oxide donors and scavengers

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