S‐nitrosylation/denitrosylation as a regulatory mechanism of salt stress sensing in sunflower seedlings

Jain, Prachi; Toerne, Christine von; Lindermayr, Christian; Bhatla, Satish C.

doi:10.1111/ppl.12641

Cited by 52 publications

(17 citation statements)

References 89 publications

(134 reference statements)

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“…In both genotypes, enolase (EC 4.2.1.11) was S-nitrosated after infection with P. infestans (Table 1). Enolase, catalysing the conversion of 2-phosphoglycerate to phosphoenolpyruvate, was previously identified as an S-nitrosation target in Arabidopsis 3,37 and sunflower 64 . In the susceptible genotype, we also detected S-nitrosated triosephosphate isomerase (EC 5.3.1.1), which catalyses a reversible conversion of glyceraldehyde-3-phosphate to dihydroxyacetone phosphate.…”

Section: S Protease Regulatorymentioning

confidence: 99%

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Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

et al. 2021

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Regulation of protein function by reversible S-nitrosation, a post-translational modification based on the attachment of nitroso group to cysteine thiols, has emerged among key mechanisms of NO signalling in plant development and stress responses. S-nitrosoglutathione is regarded as the most abundant low-molecular-weight S-nitrosothiol in plants, where its intracellular concentrations are modulated by S-nitrosoglutathione reductase. We analysed modulations of S-nitrosothiols and protein S-nitrosation mediated by S-nitrosoglutathione reductase in cultivated Solanum lycopersicum (susceptible) and wild Solanum habrochaites (resistant genotype) up to 96 h post inoculation (hpi) by two hemibiotrophic oomycetes, Phytophthora infestans and Phytophthora parasitica. S-nitrosoglutathione reductase activity and protein level were decreased by P. infestans and P. parasitica infection in both genotypes, whereas protein S-nitrosothiols were increased by P. infestans infection, particularly at 72 hpi related to pathogen biotrophy–necrotrophy transition. Increased levels of S-nitrosothiols localised in both proximal and distal parts to the infection site, which suggests together with their localisation to vascular bundles a signalling role in systemic responses. S-nitrosation targets in plants infected with P. infestans identified by a proteomic analysis include namely antioxidant and defence proteins, together with important proteins of metabolic, regulatory and structural functions. Ascorbate peroxidase S-nitrosation was observed in both genotypes in parallel to increased enzyme activity and protein level during P. infestans pathogenesis, namely in the susceptible genotype. These results show important regulatory functions of protein S-nitrosation in concerting molecular mechanisms of plant resistance to hemibiotrophic pathogens.

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Section: S Protease Regulatorymentioning

confidence: 99%

“…39 ; Begara-Morales et al4 ; Jain et al64 Defence proteins and enzymes 44 ; Jain et al64 Putative late blight resistance protein homologue R1B-16…”

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confidence: 99%

Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

et al. 2021

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“…Salt stress elevates Snitrosylation of cytosolic proteins in seedling cotyledons, while in roots, denitrosylation of proteins is observed. S-nitroglutathione reductase (GSNOR) activity is inhibited under salt stress 51,52 (Figure 2). These attributes are likely to provide longevity to oil bodies (slow mobilization) for survival of seedlings.…”

Section: Salt Stress Delays Oil Body Mobilization During Seedling Growthmentioning

confidence: 99%

Biochemical mechanisms regulating salt tolerance in sunflower

Gogna

Bhatla

2019

Plant Signaling & Behavior

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Sunflower plants are semi-tolerant to salt stress. Calcium modulates the expression of oubain-sensitive ATPases, responsible for sodium fluxes in cells. Salt stress delays degradation of oil body (OB) membrane proteins. Serotonin and melatonin contents are elevated in response to salt stress. Melatonin can detoxify the seedlings of elevated reactive oxygen species (ROS) levels. Enhanced nitric oxide (NO) expression correlates with NaCl-induced modulation of seedling growth. Salt stress enhances S-nitrosylation of cytosolic proteins in seedling cotyledons, while in roots, denitrosylation of proteins is observed. Lipid peroxide content and glutathione peroxidase (GPX4) activity are enhanced in response to salt stress. Salt stress downregulates the activity of superoxide dismutase (SOD) and upregulates the activity of GPX4 and glutathione reductase (GR). Heme oxygenase-1 (HO-1) abundance in cells surrounding the secretory canal in seedling cotyledons is enhanced in response to salt stress. NO negatively regulates the total glutathione homeostasis and regulates polyamine and glycine betaine homeostasis in response to salt stress. An intricate biochemical crosstalk is thus observed to control salt tolerance mechanisms in sunflower.

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“…NO fulfills its biological functions by modulating protein function/activity through different types of post-translational modifications (PTM): Protein S-nitrosation, tyrosine nitration or metal nitrosylation. Protein S-nitrosation -the covalent attachment of NO to the sulfur group of cysteine residues -is one of the most important NO-dependent protein modifications, and plants respond to many different environmental changes by S-nitrosating a specific set of proteins (Jain et al, 2018;Romero-Puertas et al, 2008;Vanzo et al, 2016). S-Nitrosated glutathione (S-nitrosoglutathione, GSNO) has an important function as NO reservoir, NO transporter, and physiological NO donor, which can transfer its NO moiety to protein cysteine residues (Hess et al, 2005;Kovacs and Lindermayr, 2013).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide coordinates histone acetylation and expression of genes involved in growth/development and stress response

Ageeva-Kieferle

Georgii

Winkler

et al. 2020

Preprint

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Nitric oxide (NO) is a signaling molecule with multiple regulatory functions in plant physiology and stress response. Besides direct effects on the transcriptional machinery, NO can fulfill its signaling function via epigenetic mechanisms.We report that light intensity-dependent changes in NO correlate with changes in global histone acetylation (H3, H3K9 and H3K9/K14) in Arabidopsis thaliana wild-type leaves and that this correlation depends on S-nitrosoglutathione reductase and histone deacetylase 6. The activity of histone deacetylase 6 was sensitive to NO, which demonstrates that NO participates in regulation of histone acetylation. ChIP-seq and RNA-seq analyses revealed that NO is involved in the metabolic switch from growth and development to stress response. This coordinating function of NO might be of special importance in adaptation to a changing environment and could therefore be a promising starting point to mitigating the negative effects of climate change on plant productivity.

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S‐nitrosylation/denitrosylation as a regulatory mechanism of salt stress sensing in sunflower seedlings

Cited by 52 publications

References 89 publications

Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

Biochemical mechanisms regulating salt tolerance in sunflower

Nitric oxide coordinates histone acetylation and expression of genes involved in growth/development and stress response

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