Nitric Oxide Synthase‐Like Activities in Plants

Yamasaki, Hideo; Itoh, Ryuuichi; Bouchard, Josée Nina; Dghim, Ata Allah; Hossain, Khurshida K.; Gurung, Sushma; Cohen, Michael F.

doi:10.1002/9781444328608.ch5

Cited by 2 publications

(2 citation statements)

References 109 publications

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“…The existence of NO 3 − in plant tissues is evidence for NO 3 − uptake from the soil or atmosphere because NO 3 − production in non-N 2 fixing plants is negligible under normal conditions ( 36 – 40 ). Although NO 3 − can be produced from the oxidation of nitric oxide (NO) both enzymatically and nonenzymatically in non-N 2 fixing plants ( 37 – 40 ), the rates are very low in natural environments ( 41 – 44 ), especially compared with the pool sizes of NO 3 − detected in plants of this study. Besides, while NO 3 − production by nonsymbiotic hemoglobin is possible in anoxic conditions ( 38 , 39 ) and with high ambient NO concentrations ( 40 ), neither anoxic conditions nor high ambient NO applies to the present study.…”

Section: Resultsmentioning

confidence: 99%

Nitrate is an important nitrogen source for Arctic tundra plants

Liu

Koba

Koyama

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

107

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SignificanceHow terrestrial plants use N and respond to soil N loading is central to evaluating and predicting changing ecosystem structure and function with climate warming and N pollution. Here, evidence from NO3− in plant tissues has uncovered the uptake and assimilation of soil NO3− by Arctic tundra plants, which has long been assumed negligible. Soil NO3− contributed about one-third of the bulk N used by tundra plants of northern Alaska. Accordingly, the importance of soil NO3− for tundra plants should be considered in future studies on N and C cycling in Arctic ecosystems where C sequestration is strongly determined by N availability.

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Section: Resultsmentioning

confidence: 99%

Nitrate is an important nitrogen source for Arctic tundra plants

Liu

Koba

Koyama

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

107

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“…Many physiological studies have suggested that plants produce nitrite only under stress conditions [32,33]. However, its production mechanism is still under debate [34].…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Nitrite Content in Fresh Spinach Leaves: Evidence for Nitrite Formation Caused by Microbial Nitrate Reductase Activity

Watanabe¹,

Yamasaki²

2017

J Nutr Food Sci

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Nitrite (NO 2 -) contained in dietary foods has long been recognized for its toxicity as the causative agent of methemoglobinemia and also as a source of mutagenic nitrosamines. Because of these potential toxicities, nitrite as well as nitrate contained in foods and drinks are strictly limited by regulations in many countries. Recent studies have offered us to update our recognition of nitrite; nitrite is an important precursor for Nitric Oxide (NO) that is required for fundamental physiological activities including vasorelaxation. Although it is well established that green vegetables contain high amounts of nitrate, there has been controversy regarding the source of nitrite accumulation in fresh green vegetables. In this study, we investigated the dynamics of nitrite and nitrate contents in spinach leaf extracts to verify the mechanisms of nitrite formation. The time course of nitrite production in leaf extracts showed a reciprocal relationship with nitrate degradation, suggesting a conversion from nitrate to nitrite. The reaction strongly depended on temperature and it was suppressed at a low temperature. Sodium tungstate, a nitrate reductase enzyme inhibitor, was effective to suppress the conversion. Pre-sterilization by autoclaving or filter sterilization completely prevented the formation of nitrite as well as degradation of nitrate. We suggest that previous reports of nitrite accumulation can be attributed to microbial nitrate reductase activities that occur during the degradation spinach leaves.

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Nitric Oxide Synthase‐Like Activities in Plants

Cited by 2 publications

References 109 publications

Nitrate is an important nitrogen source for Arctic tundra plants

Nitrate is an important nitrogen source for Arctic tundra plants

Dynamics of Nitrite Content in Fresh Spinach Leaves: Evidence for Nitrite Formation Caused by Microbial Nitrate Reductase Activity

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