Nitric oxide synthase inhibitor NG-monomethyl-l-arginine preserves sperm motility after swim-up

Perera, Doreen M.D.; Katz, Maurice; Heenbanda, Sujiwa R.; Marchant, Sally

doi:10.1016/s0015-0282(16)58645-2

Cited by 22 publications

(10 citation statements)

References 4 publications

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“…Consistent with this finding, higher NO levels have been reported in the semen of asthenozoospermic infertile men than those of the normozoospermic fertile counterpart (Perera et al, 1996;Balercia et al, 2004;Bolanos et al, 2008). Mitochondria play a central role in energy metabolism and regulate the sperm motility, AR, and spermatozoa-oocyte fusion (Gallon et al, 2006;Agarwal et al, 2008;Otasevic et al, 2013).…”

Section: Introductionsupporting

confidence: 54%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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SUMMARYSodium nitroprusside is a nitric oxide donor involved in the regulation of the motility, hyperactivation, capacitation, and acrosome reaction (AR) of spermatozoa. However, the molecular mechanism underlying this regulation has not yet been elucidated. Therefore, this study was designed to evaluate the molecular basis for the effects of sodium nitroprusside on different processes in spermatozoa and its consequences on subsequent oocyte fertilization and embryo development. In this in vitro study, mouse spermatozoa were incubated with various concentrations of sodium nitroprusside (1, 10, and 100 lM) for 90 min. Our results showed that sodium nitroprusside inhibited sperm motility and motion kinematics in a dose-dependent manner by significantly enhancing intracellular iron and reactive oxygen species (ROS), and decreasing Ca 2+ , and adenosine triphosphate levels in spermatozoa. Moreover, short-term exposure of spermatozoa to sodium nitroprusside increased the tyrosine phosphorylation of sperm proteins involved in PKA-dependent regulation of intracellular calcium levels, which induced a robust AR. Finally, sodium nitroprusside significantly decreased the rates of fertilization and blastocyst formation during embryo development. Based on these results, we propose that sodium nitroprusside increases ROS production and precocious AR may alter overall sperm physiology, leading to poor fertilization and compromised embryonic development.

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Section: Introductionsupporting

confidence: 54%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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“…Several reports have shown a decrease in progressive sperm motility in the presence of high concentrations of pure ˙NO (25–125 μM) and SNP (0.25–2.5 mM), or S‐nitroso‐N‐acetylpenicillamine (12–600 μM) and 3‐morpholinosydnonimine (100–125 μM) . Consistent with these findings, high ˙NO levels have been reported in the semen of infertile men with decreased sperm motility . In addition, high levels of ˙NO in spermatozoa detected in infertile men are linked to a decrease in sperm metabolism …”

Section: Pathophysiological Roles Of Rns On Male Reproductionsupporting

confidence: 60%

“…6,98-100 Consistent with these findings, high˙NO levels have been reported in the semen of infertile men with decreased sperm motility. 98,112,113 In addition, high levels of˙NO in spermatozoa detected in infertile men are linked to a decrease in sperm metabolism. 80 Similar to sperm motility, it has been shown that in high concentrations˙NO induces atypical sperm morphology 114 and negatively impacts sperm viability.…”

Section: Pathophysiological Roles Of Rns On Male Reproductionmentioning

confidence: 99%

Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

et al. 2019

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Infertility is a significant global health problem that currently affects one of six couples in reproductive age. The quality of male reproductive cells dramatically decreased over the last years and almost every aspect of modern life additionally worsen sperm functional parameters that consequently markedly increase male infertility. This clearly points out the importance of finding a new approach to treat male infertility. Redox signaling mediated by reactive oxygen, nitrogen and sulfur species (ROS, RNS, and RSS respectively), has appeared important for sperm reproductive function. Present review summarizes the current knowledge of ROS, RNS, and RSS in male reproductive biology and identifies potential targets for development of novel pharmacological and therapeutic approaches for male infertility by targeted therapeutic modulation of redox signaling.

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“…Osteoclasts, which are themselves producers of NO, require a minimal basal amount of NO for their normal function, but high NO concentrations, such as those produced in in¯ammatory bone and joint diseases, impair their function (Brandi et al, 1995). Similarly, low levels of NO exert a stimulatory effect on sperm motility, but this switches to inhibitory in the presence of high NO levels (Hellstrom et al, 1994;Perera et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Biphasic effect of nitric oxide on testosterone and cyclic GMP production by purified rat Leydig cells cultured in vitro

et al. 1999

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Nitric oxide (NO) biphasically modulates osteoclast function and sperm motility by exerting a positive effect at low concentrations and a negative effect at high concentrations. We therefore tested whether NO exerts a comparable effect on testosterone secretion by cultured rat Leydig cells. Three NO-donors, S-nitroso-N-acetylpenicillamine (SNAP), diethylamine/nitric oxide complex sodium salt (DEA/NO) and diethylenetriamine nitric oxide adduct (DETA/NO) were administered in a wide range of concentrations (10(-8)-10(-3) M for 3 h) to Percoll-purified Leydig cells from adult rats. These drugs raised testosterone and cGMP secretion when used at low concentrations (10(-8)-10(-5) M); however, they inhibited testosterone, but did not affect cGMP, secretion at concentrations higher than 10(-5) M. Administration of the NO scavenger haemoglobin (160 micrograms/mL) prevented both the stimulatory and the inhibitory effect of these drugs. Nitrite accumulation was measured as a marker of NO released by the drugs in our in vitro system; it fell within the range of control media in the presence of NO-donor concentrations lower than 10(-5) M, but was several-fold higher in the media of cells treated with concentrations of the NO-donors greater than 10(-5) M. These data show that (1) NO exerts a biphasic effect on testosterone secretion, which is stimulatory at low and inhibitory at high concentrations; (2) the stimulatory effect of NO is mediated by cGMP, the classic second messenger for NO action.

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Nitric oxide synthase inhibitor NG-monomethyl-l-arginine preserves sperm motility after swim-up

Cited by 22 publications

References 4 publications

Sodium nitroprusside suppresses male fertility in vitro

Sodium nitroprusside suppresses male fertility in vitro

Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

Biphasic effect of nitric oxide on testosterone and cyclic GMP production by purified rat Leydig cells cultured in vitro

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