Nitric oxide-scavenging activity of polyhydroxylated fullerenol, C60(OH)24

Mirkov, Snezana; Djordjević, Aleksandar; Andric, Nebojsa L.; Andrić, Silvana A.; Kostic, Tatjana S.; Bogdanovic, Gordana; Vojinovic-Miloradov, Mirjana; Kovačević, Radmila

doi:10.1016/j.niox.2004.08.003

Cited by 187 publications

(151 citation statements)

References 31 publications

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“…Among numerous reported fullerene derivatives, nanofullerol is the most extensively studied, and has been implicated as a powerful antioxidant in the biomedical field. [48][49][50][51][52][53][54][55][56] Therefore, fullerol was selected in our present study.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative nanofullerol prevents intervertebral disk degeneration

Yang¹,

Jin²,

Lu³

et al. 2014

IJN

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Compelling evidence suggests that reactive oxygen species (ROS) play a pivotal role in disk degeneration. Fullerol nanoparticles prepared in aqueous solution have been demonstrated to have outstanding ability to scavenge ROS. In this report, in vitro and in vivo models were used to study the efficacy of fullerol in preventing disk degeneration. For in vitro experiments, a pro-oxidant H 2 O 2 or an inflammatory cytokine interleukin (IL)-1β was employed to induce degenerated phenotypes in human nucleus pulposus cells encapsulated in alginate beads, and fullerol was added in the culture medium. For the animal study, an annulus-puncture model with rabbit was created, and fullerol was injected into disks. It was shown that cytotoxicity and cellular ROS level induced by H 2 O 2 were significantly diminished by fullerol. IL-1β-induced nitric oxide generation in culture medium was suppressed by fullerol as well. Gene-profile and biochemical assays showed that fullerol effectively reversed the matrix degradation caused by either H 2 O 2 or IL-1β. The animal study delineated that intradiskal injection of fullerol prevented disk degeneration, increasing water and proteoglycan content and inhibiting ectopic bone formation. These results suggest that antioxidative fullerol may have a potential therapeutic application for disk degeneration.

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Section: Discussionmentioning

confidence: 99%

Antioxidative nanofullerol prevents intervertebral disk degeneration

Yang¹,

Jin²,

Lu³

et al. 2014

IJN

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“…[1][2][3][4] Polyhydroxylated fullerene, known as fullerenol (C 60 (OH) x ), has attracted considerable attention due to its antioxidative [5][6][7][8] and antiproliferative activity. 9,10 Hence, several studies reported that fullerenol and other fullerene derivatives may be used as drug carriers, 11,12 neuroprotective agents, 13,14 bioimaging contrast agents, [15][16][17][18][19] and anticancer drugs.…”

Section: Introductionmentioning

confidence: 99%

“…A previous study showed that fullerenol directly scavenged NO. 7 Other studies showed that fullerene derivatives such as tris-malonyl-C 60 also inhibited NOS activity and reduced the maximal velocity of product formation in rat pituitary cells in vitro. 51 Fullerenol-induced reduction of NO synthesis and scavenging of NO may mediate the suppression of synaptic plasticity.…”

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confidence: 97%

Suppression of synaptic plasticity by fullerenol in rat hippocampus in vitro

Wang¹,

Zha²,

Yang³

et al. 2016

IJN

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Fullerenol, a water-soluble fullerene derivative, has attracted much attention due to its bioactive properties, including the antioxidative properties and free radical scavenging ability. Due to its superior nature, fullerenol represents a promising diagnostic, therapeutic, and protective agent. Therefore, elucidation of the possible side effects of fullerenol is important in determining its potential role. In the present study, we investigated the acute effects of 5 μM fullerenol on synaptic plasticity in hippocampal brain slices of rats. Incubation with fullerenol for 20 minutes significantly decreased the peak of paired-pulse facilitation and long-term potentiation, indicating that fullerenol suppresses the short- and long-term synaptic plasticity of region I of hippocampus. We found that fullerenol depressed the activity and the expression of nitric oxide (NO) synthase in hippocampus. In view of the important role of NO in synaptic plasticity, the inhibition of fullerenol on NO synthase may contribute to the suppression of synaptic plasticity. These findings may facilitate the evaluation of the side effects of fullerenol.

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“…We hypothesize that an antioxidant would favor osteogenic differentiation in ADSCs as well. Here, we investigated an extremely powerful antioxidant, polyhydroxylated fullerene (fullerol), [17][18][19] and report for the first time that antioxidative fullerol enhanced osteogenesis and reduced reactive oxygen species (ROS) in human ADSCs, as well as increased expression of FoxO1, a major isoform of forkhead box O transcription factors that defend against ROS and promote osteoblast differentiation in bone.…”

Section: Introductionmentioning

confidence: 99%

“…31 Our present data showed that fullerol served as an antioxidant in human ADSCs without cytotoxicity at doses up to 10 µM (Figures 1 and 2), and this is in agreement with a majority of previous reports with regards to other tissues and cell types. [17][18][19]28,[32][33][34][35][36][37][38][39] It has been shown that ROS inhibit osteogenesis and bone formation. Mody et al 40 reported that three oxidants, hydrogen peroxide (H 2 O 2 ), xanthine, and xanthine oxidase, and minimally oxidized low-density lipoproteins inhibited osteoblastic differentiation of MC3T3-E1 mouse bone preosteoblast cell line and M2-10B4 mouse marrow stromal cell line, while increasing intracellular oxidative stress.…”

mentioning

confidence: 99%

Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

Yang¹,

Li²,

Wan³

et al. 2014

IJN

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Antioxidants were implicated as potential reagents to enhance osteogenesis, and nano-fullerenes have been demonstrated to have a great antioxidative capacity by both in vitro and in vivo experiments. In this study, we assessed the impact of a polyhydroxylated fullerene, fullerol, on the osteogenic differentiation of human adipose-derived stem cells (ADSCs). Fullerol was not toxic against human ADSCs at concentrations up to 10 μM. At a concentration of 1 μM, fullerol reduced cellular reactive oxygen species after a 5-day incubation either in the presence or in the absence of osteogenic media. Pretreatment of fullerol for 7 days increased the osteogenic potential of human ADSCs. Furthermore, when incubated together with osteogenic medium, fullerol promoted osteogenic differentiation in a dose-dependent manner. Finally, fullerol proved to promote expression of FoxO1, a major functional isoform of forkhead box O transcription factors that defend against reactive oxygen species in bone. Although further clarification of related mechanisms is required, the findings may help further development of a novel approach for bone repair, using combined treatment of nano-fullerol with ADSCs.

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Nitric oxide-scavenging activity of polyhydroxylated fullerenol, C60(OH)24

Cited by 187 publications

References 31 publications

Antioxidative nanofullerol prevents intervertebral disk degeneration

Antioxidative nanofullerol prevents intervertebral disk degeneration

Suppression of synaptic plasticity by fullerenol in rat hippocampus in vitro

Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

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