Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

Abstract

Antioxidants were implicated as potential reagents to enhance osteogenesis, and nano-fullerenes have been demonstrated to have a great antioxidative capacity by both in vitro and in vivo experiments. In this study, we assessed the impact of a polyhydroxylated fullerene, fullerol, on the osteogenic differentiation of human adipose-derived stem cells (ADSCs). Fullerol was not toxic against human ADSCs at concentrations up to 10 μM. At a concentration of 1 μM, fullerol reduced cellular reactive oxygen species after a 5-day incubation either in the presence or in the absence of osteogenic media. Pretreatment of fullerol for 7 days increased the osteogenic potential of human ADSCs. Furthermore, when incubated together with osteogenic medium, fullerol promoted osteogenic differentiation in a dose-dependent manner. Finally, fullerol proved to promote expression of FoxO1, a major functional isoform of forkhead box O transcription factors that defend against reactive oxygen species in bone. Although further clarification of related mechanisms is required, the findings may help further development of a novel approach for bone repair, using combined treatment of nano-fullerol with ADSCs.

Anti‑aging effect of fullerenol on skin aging through derived stem cells in a mouse model

“…OCN is a common signal molecule that promotes osteogenesis and bone inducing factors (17). OCN is a common signal molecule to promote osteogenesis of bone inducing factors (18). Therefore, the present study identified that treatment with fullerenol significantly increased the expression of ALP and OCN in D-galactose-induced mice (P<0.05).…”

“…Therefore, the present study identified that treatment with fullerenol significantly increased the expression of ALP and OCN in D-galactose-induced mice (P<0.05). Yang et al (18) reported that fullerenol promotes osteogenesis of human ADSCs through Runx2, ALP and OCN.…”

“…The present study used treatment with fullerenol and observed a significant increase in the D-galactose-induced inhibition of FoxO1 protein expression in mice. Yang et al (18) reported that fullerenol promotes osteogenesis of human ADSCs through promotion of FOXO4.…”

Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression

“…20,21 Our previous study showed that the CNTs can accelerate gap junction formation remarkably via activation of the β1-integrinmediated FAK/ERK/GATA4 pathway. 22 Yang et al 23 found that the 0D fullerol enhances the osteogenesis of human adipose-derived stem cells. Despite these exciting findings, relatively little is known about the molecular mechanism of fullerene-C 60 in proliferation and cardiomyogenic differentiation of MSCs.…”

“…37 Fullerene-C 60 treatment partially improved the DNA synthesis in cells, resulting in higher cell number and proliferation. 23,38 The MTT assay was conducted to measure cell proliferation and viability following exposure to fullerene-C 60 for 7 days. As shown in Figure 4C, the cells proliferated constantly in all the groups.…”