Nitric Oxide Production Disorders in Leukocytes of Patients With Recurrent Furunculosis

Abstract

Aim. The prop ensity for certain individuals to develop staphylococcal recurrent furunculosis (RF) is not fully understood. But among the reasons of its development the immune system dysfunctions are described. As in the staphylococcus elimination the main role is played by neutrophils, the objective of this study was to determine nitric oxide (NO) and reactive oxygen species (ROS) production by polymo rphonuclear leukocytes (PMNs) of patients with RF and to compare them with the response of normal cells to stimulation.Materials and methods. The spontaneous and pyrogenal-stimulated nitric oxide production was evaluated in leukocyte cell cultures by Griess reaction, and ROS generation was determined in the stimulated and unstimulated NBT-test.Results. In this study we have demonstrated that leukocytes of healthy subjects respond on stimulation by the increase of both NO and ROS production. In contrast, leukocytes of patients with RF react by depression of NO formation at stimulation, and are characterized by decrease of ROS production reserve with the increasing of spontaneous ROS generation. Described disorders are revealed in the remission period as well as at exacerbation of furunculosis.Conclusion. The present study suggests that leukocytes of patients with RF have stable defect of stimulated NO production increase, which can be the reason for recurrent and severe course of furunculosis.

Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions

“…2.7. Respiratory burst NBT reduction test was performed as described previously with some modifications (Müller et al, 1981;Nabi et al, 2005;Hamaliaka and Novikova, 2010). In brief, 200 µL of neutrophil suspension (25 × 10 5 cell/mL) was mixed with 200 µL/mL of S. aureus suspension (10 8 cells/mL) and 200 µL of 0.1% NBT in PBS (pH 7.4).…”

“…Bacterial ingestion was assayed by light microscopy under oil immersion. Phagocytosis activities of neutrophils were expressed as percentage of neutrophils that internalized at least one S. aureus (Hamaliaka and Novikova, 2010). 2.7.…”

Caffeine alters the effects of bone marrow-derived mesenchymal stem cells on neutrophils

“…The NBT reduction assay was used to measure the reactive oxygen species (ROS) activity in neutrophils. 22 The obtained findings expressed that the respiratory burst of neutrophils was significantly decreased in neutrophils co-cultured with the MSCs pulsed or without caffeine, compared to neutrophils without treatment (Fig. 4).…”

“…22 In addition to encountering pathogens, different stimuli may induce the respiratory burst in neutrophils. 27 Nonetheless, when the production of ROS is excessive or inappropriate, ROS participate in severe host tissue damages and in different immunopathological conditions.…”

<b><i>Staphylococcus aureus</i></b> and Host Immunity in Recurrent Furunculosis

“…They revealed that impaired chemotaxis appears only in patients with recurrent furunculosis and can serve as an independent risk factor for recurrent furunculosis. Hamaliaka and Novikova [66] demonstrated depression of nitric oxide formation at stimulation and a decrease in the production of reactive oxygen species in 58 patients suffering from recurrent furunculosis.…”