Nitric oxide production by nurse shark (Ginglymostoma cirratum) and clearnose skate (Raja eglanteria) peripheral blood leucocytes

Walsh, Catherine J.; Toranto, Jason D.; Gilliland, C. Taylor; Noyes, David; Bodine, A.B.; Luer, Carl A.

doi:10.1016/j.fsi.2005.03.011

Cited by 17 publications

(10 citation statements)

References 27 publications

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“…RNI like NO are important modulators of the vertebrate immune system (Neumann et al . ; Walsh, Toranto, Gilliland, Noyes, Bodine & Luer ). This study indicated that the NOQAF of C. scalpelliformis has significantly increased the RNI production on most of the days tested.…”

Section: Discussionmentioning

confidence: 99%

Immunostimulatory effects ofN-Oxide-Quaternary alkaloid fraction of a marine Chlorophycean macroalga in the striped murrel,Channa striata(Bloch)

Balasubramanian¹,

Michael

2014

Aquac Res

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N-Oxide-Quaternary Alkaloid Fraction (NOQAF) of a Chlorophycean macroalga, Caulerpa scalpelliformis (R.Br.) C. Ag f. denticulata (Deaisne) Weber van Bosse (Patent filed) was intraperitoneally administered at different doses (0, 2, 20 or 200 mg kg À1 body weight) in different groups of Channa striata to assess its effect on the non-specific immune responses and disease resistance against live virulent Aeromonas hydrophila. A reference positive control [MacroGard (Biotec Pharmacon ASA, Norway) 20 mg kg À1 body weight] was simultaneously maintained to compare its efficacy. The fraction (NOQAF) has been found to enhance the serum lysozyme, peroxidase, antiprotease and alternate complement activities and the reactive oxygen and nitrogen species production and peroxidase activity by the peripheral blood leucocytes than the control group during the study period. On experimental challenge with live virulent A. hydrophila, maximum RPS values of 88.8 and 87.4 were noted in the groups treated with single dose of 20 mg kg À1 and double dose of 2 mg kg À1 of NOQAF respectively. In conclusion, the intraperitoneal administration of the N-Oxide-Quaternary Alkaloid Fraction (NOQAF) of C. scalpelliformis has been found to significantly enhance the non-specific immune responses and disease resistance against A. hydrophila in Channa striata.

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Section: Discussionmentioning

confidence: 99%

Immunostimulatory effects ofN-Oxide-Quaternary alkaloid fraction of a marine Chlorophycean macroalga in the striped murrel,Channa striata(Bloch)

Balasubramanian¹,

Michael

2014

Aquac Res

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“…Nitric oxide (NO) production after 24 h of culture in the presence of 1, 10 or 100 μg/ml levamisole and controls was determined using a procedure modifi ed from Walsh et al (2006), based on detection of nitrite (NO 2 -) derived from the nitric oxide (NO) by means of the Griess reagent (sulphanilamide, naphthylethylenediamine and phosphoric acid) (Sigma-Aldrich Co., St. Louis., MO., USA).…”

Section: Production Of Ros and No By Macrophagesmentioning

confidence: 99%

In vitro effect of Levamisole on Rainbow Trout (Oncorhynchus mykiss) peripheral blood mononuclear cells

2015

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The in vitro effect of levamisole (1, 10 and 100 μg/ml) on cells of the innate immune response of rainbow trout (Oncorhynchus mykiss) was studied, particularly the activity of natural cytotoxic cells and macrophages present in peripheral blood. Levamisole effect on natural cytotoxic cells was evaluated by their capacity to lyse HL-60 tumor cells while activity of macrophages was evaluated measuring production of reactive oxygen species and nitric oxide. Results indicate that natural cytotoxic cells activity was signifi cantly increased by 1 μg/ml levamisole and that higher doses had no effect. On the other hand, reactive oxygen species and nitric oxide production by macrophages was not increased by any of the levamisole doses used and that the higher dose (100 μg/ml) induced a signifi cant decrease of nitric oxide levels, which might be deleterious for the defensive response of these fi sh. KEYWORDS:Innate immunity; reactive oxygen species; ROS; nitric oxide. RESUMENSe estudió el efecto in vitro del levamisol (1, 10 y 100 μg/ml) sobre células de la respuesta inmune innata de trucha arcoíris (Oncorhynchus mykiss), particularmente la actividad de las células citotóxicas naturales y de los macrófagos presentes en sangre periférica. El efecto del levamisol sobre las células citotóxicas naturales fue evaluado mediante su capacidad para lisar células tumorales HL-60, mientras que la actividad de los macrófagos fue evaluado mediante la producción de especies reactivas del oxígeno y de óxido nítrico. Los resultados indican que la actividad de las células citotóxicas naturales fue incrementada signifi cativamente por 1 μg/ml levamisol mientras que dosis mayores no produjeron este efecto. Por otro lado, la producción de especies reactivas del oxígeno y de óxido nítrico por los macrófagos no fue incrementada por ninguna de las dosis usadas, sin embargo dosis de 100 μg/ml indujeron una disminución signifi cativa en los niveles de óxido nítrico, lo cual podría ser deletéreo para la respuesta defensiva de estos peces. PALABRAS CLAVES:Inmunidad innata; especies reactivas del oxígeno; ROS; óxido nítrico.

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“…Assays were done in triplicate. The NO production was assessed at times 1, 24 and 48 h after stimulation with PHA or LPS using a procedure modifi ed from Walsh et al (2006) using Griess reagent (sulfanilamide, N-naphthyl ethylenediamine and phosphoric acid) (SigmaAldrich Co., St. Louis., MO., USA).…”

Section: Activity Of Nonspecific Cytotoxic Cells (Nccs)mentioning

confidence: 99%

“…Macrophages are mononuclear phagocytic cells; they eliminate microorganisms by means of several mechanisms, including phagocytosis and the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) such as nitric oxide (NO). Both, ROS and NO are highly toxic compounds for phagocytosed microorganisms (Walsh et al 2006;Lundén et al 2002;Secombes 1996) allowing macrophages to destroy them.…”

Section: Introductionmentioning

confidence: 99%

Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)

Gómez¹,

Cortés²,

Smith³

2013

Gayana (Concepc.)

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This work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecifi c cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells of mammals, capable of eliminating cells infected with intracellular pathogens and also tumoral cells. Their lytic activity is mediated by the perforin/granzyme system and the Fas receptor-Fas ligand (FasR-FasL) system. Activity of NCCs was evaluated by their capacity to lyse HL-60 tumor cells. On the other hand, macrophages eliminate microorganisms by several mechanisms, including the production of reactive oxygen species (ROS) and nitric oxide (NO). Results showed that PHA signifi cantly increased NCCs cytotoxic activity against HL-60 cells, even under conditions in which the perforins/granzymes lytic system should be suppressed, supporting the existence of the FasR-FasL system in trouts. PHA showed no effect on ROS and NO production by macrophages. Finally, LPS showed no effect on the cytotoxic activity of NCCs or in the production of ROS and NO by macrophages. KEYWORDS:Interleukins, innate immunity, FasR-FasL, lectins, leukocytes. RESUMENEn este trabajo evaluamos el efecto de la lectina fi tohemaglutinina (PHA) y del lipopolisacárido bacteriano (LPS) sobre la actividad de las células citotóxicas naturales (NCCs) y de los macrófagos de la sangre periférica de la truchas arcoíris. Las NCCs son células de la inmunidad innata, homologas a las células Natural Killer (NK) de mamíferos, capaces de eliminar células infectadas con patógenos intracelulares y también células tumorales. Su actividad lítica es mediada por las perforinas/granzymas y el sistema Fas receptor-Fas Ligando (FasR-FasL). La actividad de estas células fue evaluada por su capacidad para lisar células tumorales HL-60. Por otro lado, los macrófagos eliminan microrganismos mediante diversos mecanismos, los que incluyen la producción de especies reactivas del oxígeno (ROS) y del óxido nítrico (NO). Nuestros resultados muestran que el PHA incrementa signifi cativamente la actividad de las NCCs contra las células HL-60, aún bajo condiciones en las que el sistema lítico de las perforinas/granzimas estaría suprimido, apoyan la existencia del sistema FasR-FasL en truchas. El PHA no mostró efecto alguno en la producción de ROS y NO por los macrófagos. Finalmente, al evaluar el efecto del LPS en ambas poblaciones celulares, no se obtuvo efecto alguno tanto en la actividad citotóxica de las NCCs, como tampoco en la producción de ROS y NO por los macrófagos. PALABRAS CLAVE:Interleuquinas, inmunidad innata, FasR-FasL, lectinas, leucocitos.Gayana 77(1): 53-59, 2013.

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Nitric oxide production by nurse shark (Ginglymostoma cirratum) and clearnose skate (Raja eglanteria) peripheral blood leucocytes

Cited by 17 publications

References 27 publications

Immunostimulatory effects ofN-Oxide-Quaternary alkaloid fraction of a marine Chlorophycean macroalga in the striped murrel,Channa striata(Bloch)

Immunostimulatory effects ofN-Oxide-Quaternary alkaloid fraction of a marine Chlorophycean macroalga in the striped murrel,Channa striata(Bloch)

In vitro effect of Levamisole on Rainbow Trout (Oncorhynchus mykiss) peripheral blood mononuclear cells

Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)

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