Nitric Oxide Participates in the Complex Interplay of Defense-Related Signaling Pathways Controlling Disease Resistance to <i>Sclerotinia sclerotiorum</i> in <i>Arabidopsis thaliana</i>

Perchepied, Laure; Balagué, Claudine; Riou, Catherine; Claudel‐Renard, Clotilde; Rivière, Nathalie; Grezes-Besset, Bruno; Roby, Dominique

doi:10.1094/mpmi-23-7-0846

Cited by 153 publications

(138 citation statements)

References 98 publications

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“…NO a small diffusible molecule is nowadays being recognized as an emerging key signaling molecule in response to broad range of stresses in plants (Neill et al 2003;RomeroPuertas et al 2004;Khan et al 2012). It appears to be an early signaling factor that helps in orchestrating a number of downstream signaling pathways (Perchepied et al 2010). From our laboratory we have reported the correlation between the NO generation and induction of defenserelated molecules due to application of elicitor on tea leaves (Chandra et al 2014b.…”

Section: Discussionmentioning

confidence: 99%

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Chakraborty

Ghosh²,

Chandra

et al. 2016

Physiol Mol Biol Plants

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Improvement of the host resistance by using hazard free chemical elicitors is emerging as an alternative approach in the field of plant disease management. In our present work, we have screened the efficacy and possible mechanism of abiogenic elicitors like Dipotassium hydrogen orthophosphate (K 2 HPO 4 ), Oxalic acid (OA), Isonicotinic acid (INA), Salicylic acid (SA), Acetylsalicylate (AS), Arachidonic acid (AA) and Calcium chloride (CaCl 2 ) to stimulate innate immune responses in Lycopersicum esculentum Mill. Excised tomato leaves, treated with elicitors at three different concentrations, were found to stimulate defense and antioxidative enzymes, total phenol and flavonoid content after 24 h of incubation. CaCl 2 (0.5 %) followed by INA (2.5 mM) were found most effective in activation of all such defense molecules in tomato leaves. Furthermore, nitric oxide (NO), a key gaseous mediator in plant defense signaling, was also measured after subsequent elicitor application. Higher doses of elicitors showed an elevated level of reactive oxygen species (ROS) generation, enhanced lipid peroxidation rate and proline content, which indicates the extent of abiotic stress generation on the leaves. However, ROS production, lipid peroxidation rate and proline concentration remain significantly reduced as a result of CaCl 2 (0.5 %) and INA (2.5 mM) application. A sharp increase of total chlorophyll content was also recorded due to treatment of CaCl 2 (0.5 %). These results demonstrate the effects of different abiogenic elicitors to regulate the production of defense molecules. Results also suggest that among all such chemicals, CaCl 2 (0.5 %) and INA (2.5 mM) can be used as a potential elicitor in organic farming of tomato.

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Section: Discussionmentioning

confidence: 99%

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Chakraborty

Ghosh²,

Chandra

et al. 2016

Physiol Mol Biol Plants

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“…A link between disease resistance, NO and ROS was also indicated by analysing different Arabidopsis ecotypes after inoculation with Sclerotinia sclerotiorum. Resistant ecotypes showed a higher expression of AtRbohD and F as well as an earlier accumulation of NO and H 2 O 2 when compared to susceptible ecotypes during interaction with S. sclerotiorum (Perchepied et al, 2010). Adding another layer of complexity to the interplay between NO and ROS, AtRbohD function was shown to be negatively regulated by NO-dependent S-nitrosylation during hypersensitive response (Yun et al, 2011).…”

Section: Ros and Resistance To Fungal Pathogens Of Leavesmentioning

confidence: 98%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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“…Microarray results indicate that S. sclerotiorum infection induces the expression of genes encoding components of diverse biological processes, including the jasmonic acid (JA) and ethylene (ET) signaling pathways (Zhao et al, 2007(Zhao et al, , 2009. Recent studies of Arabidopsis (Arabidopsis thaliana) mutants revealed that JA, ET, auxin, abscisic acid, nitric oxide, and reactive oxygen species all contribute to basal resistance against S. sclerotiorum (Guo and Stotz, 2007;Perchepied et al, 2010;Stotz et al, 2011). Conclusions in these studies about the role of the salicylic acid (SA) signaling pathway in resistance to S. sclerotiorum are contradictory (Guo and Stotz, 2007;Perchepied et al, 2010;Wang et al, 2012;Nováková et al, 2014).…”

mentioning

confidence: 99%

“…Recent studies of Arabidopsis (Arabidopsis thaliana) mutants revealed that JA, ET, auxin, abscisic acid, nitric oxide, and reactive oxygen species all contribute to basal resistance against S. sclerotiorum (Guo and Stotz, 2007;Perchepied et al, 2010;Stotz et al, 2011). Conclusions in these studies about the role of the salicylic acid (SA) signaling pathway in resistance to S. sclerotiorum are contradictory (Guo and Stotz, 2007;Perchepied et al, 2010;Wang et al, 2012;Nováková et al, 2014). Nevertheless, these results suggest that the Arabidopsis basal resistance against S. sclerotiorum is complex and involves multiple signaling pathways.…”

mentioning

confidence: 99%

The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum

Wang

Yao

et al. 2015

Plant Physiol.

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Although Sclerotinia sclerotiorum is a devastating necrotrophic fungal plant pathogen in agriculture, the virulence mechanisms utilized by S. sclerotiorum and the host defense mechanisms against this pathogen have not been fully understood. Here, we report that the Arabidopsis (Arabidopsis thaliana) Mediator complex subunit MED16 is a key component of basal resistance against S. sclerotiorum. Mutants of MED16 are markedly more susceptible to S. sclerotiorum than mutants of 13 other Mediator subunits, and med16 has a much stronger effect on S. sclerotiorum-induced transcriptome changes compared with med8, a mutation not altering susceptibility to S. sclerotiorum. Interestingly, med16 is also more susceptible to S. sclerotiorum than coronatine-insensitive1-1 (coi1-1), which is the most susceptible mutant reported so far. Although the jasmonic acid (JA)/ethylene (ET) defense pathway marker gene PLANT DEFENSIN1.2 (PDF1.2) cannot be induced in either med16 or coi1-1, basal transcript levels of PDF1.2 in med16 are significantly lower than in coi1-1. Furthermore, ET-induced suppression of JA-activated wound responses is compromised in med16, suggesting a role for MED16 in JA-ET cross talk. Additionally, MED16 is required for the recruitment of RNA polymerase II to PDF1.2 and OCTADECANOID-RESPONSIVE ARABIDOPSIS ETHYLENE/ETHYLENE-RESPONSIVE FACTOR59 (ORA59), two target genes of both JA/ETmediated and the transcription factor WRKY33-activated defense pathways. Finally, MED16 is physically associated with WRKY33 in yeast and in planta, and WRKY33-activated transcription of PDF1.2 and ORA59 as well as resistance to S. sclerotiorum depends on MED16. Taken together, these results indicate that MED16 regulates resistance to S. sclerotiorum by governing both JA/ET-mediated and WRKY33-activated defense signaling in Arabidopsis.

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Nitric Oxide Participates in the Complex Interplay of Defense-Related Signaling Pathways Controlling Disease Resistance to Sclerotinia sclerotiorum in Arabidopsis thaliana

Cited by 153 publications

References 98 publications

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Reactive oxygen species and plant resistance to fungal pathogens

The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum

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