Nitric oxide (NO) expression during annual reproductive activity in buffalo epididymis: A histochemical and immunocytochemical study

Scala, G; Maruccio, Lucianna

doi:10.1016/j.theriogenology.2012.01.017

Cited by 8 publications

(14 citation statements)

References 31 publications

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“…A reduced presence of the AQP9 was observed in the cauda region of adult orchiectomized rats (Badran and Hermo ). Our results are in line with a previous report in which season‐depending molecular changes were observed in the lining epithelium of buffalo epididymis (Scala and Maruccio ).…”

Section: Discussionsupporting

confidence: 93%

“…A reduced presence of the AQP9 was observed in the cauda region of adult orchiectomized rats (Badran and Hermo 2002). Our results are in line with a previous report in which season-depending molecular changes were observed in the lining epithelium of buffalo epididymis (Scala and Maruccio 2012). As regards the aged buffalo, the epithelium lining the cauda epididymis showed dramatically degenerated aspects, with large vacuoles inside the epithelial cells.…”

Section: Discussionsupporting

confidence: 93%

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Seasonal and Ageing‐Depending Changes of Aquaporins 1 and 9 Expression in the Genital Tract of Buffalo Bulls (Bubalus bubalis)

Arrighi

Bosi

Accogli

et al. 2016

Reprod Domestic Animals

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The presence of Aquaporins 1 (AQP1) and 9 (AQP9), integral membrane water channels that facilitate rapid passive movement of water and solutes, was immunohistochemically detected in the excurrent ducts collected from sexually mature buffalo bulls of proven fertility during the mating (late autumn-winter) and non-mating (late spring to the beginning of autumn) seasons. Furthermore, the research was performed also on the epididymal cauda of a senile buffalo bull with inactive testis. Aquaporins 1 and 9 were immunolocalized at distinct levels. In the efferent ducts, AQP1 immunoreactivity was strongly evidenced at the apical surface of the non-ciliated cells and weakly along the basal membrane of the epithelial cells. The latter reactivity disappeared during the non-mating season. No AQP1 immunoreactivity was detected in the epithelium of epididymis and vas deferens, whereas AQP1 was expressed in the smooth muscle layer of the vas deferens. Aquaporin 1 was present in the blood vessels and in small nerve bundles all along the genital tract. The supranuclear zone of the epididymal principal cells was AQP9 immunoreactive, limited to the corpus and cauda regions, and vas deferens. The samples collected in the two reproductive seasons showed a weaker AQP9 immunoreactivity during the non-mating season. A typical AQP9 immunoreactivity was noticed in the old buffalo examined. The tested AQP molecules showed a different expression pattern in comparison with laboratory mammals, primates, equine, dog and cat. In addition, seasonal differences were noticed which are possibly useful in regard to the comprehension of the morphophysiology of reproduction in the bubaline species, which are still a matter of debate.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Seasonal and Ageing‐Depending Changes of Aquaporins 1 and 9 Expression in the Genital Tract of Buffalo Bulls (Bubalus bubalis)

Arrighi

Bosi

Accogli

et al. 2016

Reprod Domestic Animals

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show abstract

“…The NO concentration changes during the annual reproductive cycle in male adult buffalo, whereby NO is mainly present in the caput epididymis during short photoperiods coinciding with maximum gonadal activity [140]. According to this, and considering the influence of melatonin on the seasonal reproduction in these animals, we can suggest the potential use of melatonin to modulate NO levels to increase buffalo fertility or in other seasonal breeds, both during semen storage or IVF.…”

Section: Potential Use Of Melatonin Against Nitrosative Stress Durmentioning

confidence: 99%

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on Gamete Handling and In Vitro Embryo Production in Humans and Other Mammals

Loren

Sánchez

Arias

et al. 2017

IJMS

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Oxidative and nitrosative stress are common problems when handling gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because their absence or overproduction causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during in vitro fertilization (IVF) has deleterious or positive effects, depending on the concentration used in the culture medium, demonstrating the delicate balance between antioxidant and pro-oxidant activity. Further research is needed to better understand the possible impact of melatonin on the different IVP steps in humans and other mammals, especially in seasonal breeds where this neuro-hormone system highly regulates its reproduction physiology.

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“…Also, NO is one of the factors that changes during the annual reproductive cycle of the male adult buffalo: NO is mainly present in the caput epididymis during short photoperiods coinciding with maximum gonadal activity [130]. According to this, and considering the influence of melatonin on seasonal reproduction in these animals we can suggest the potential use of melatonin to modulate NO levels to increase buffalo fertility or in other seasonal breeders, both during semen storage or IVF.…”

Section: Potential Use Of Melatonin Against Nitrosative Stress Duringmentioning

confidence: 97%

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on <em>In Vitro</em> Embryo Production in Mammals

Loren¹,

Sánchez²,

Arias³

et al. 2017

Preprint

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Oxidative and nitrosative stress are a common problem when manipulating gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because its absence or over production causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during IVF has deleterious or positive effects depending on the concentration used in culture medium, demonstrating the delicate balance that must exist between antioxidant and pro-oxidant activity. Further research is needed to better understand the possible impact of melatonin on the different IVP steps in domestic animals, especially in seasonal breeds where this neuro-hormone system highly regulates its reproduction physiology.

show abstract

Nitric oxide (NO) expression during annual reproductive activity in buffalo epididymis: A histochemical and immunocytochemical study

Cited by 8 publications

References 31 publications

Seasonal and Ageing‐Depending Changes of Aquaporins 1 and 9 Expression in the Genital Tract of Buffalo Bulls (Bubalus bubalis)

Seasonal and Ageing‐Depending Changes of Aquaporins 1 and 9 Expression in the Genital Tract of Buffalo Bulls (Bubalus bubalis)

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on Gamete Handling and In Vitro Embryo Production in Humans and Other Mammals

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on <em>In Vitro</em> Embryo Production in Mammals

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