Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on &lt;em&gt;In Vitro&lt;/em&gt; Embryo Production in Mammals

Loren, Pía; Sánchez, Raúl; Arias, María Elena; Felmer, Ricardo; Risopatrón, Jennie; Cheuquemán, C.

doi:10.20944/preprints201703.0195.v1

Cited by 14 publications

(1 citation statement)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are significant differences between the in vivo microenvironment and in vitro culture (IVC) systems. Increased oxygen partial pressure may be one of the main reasons for the increased oxidative stress in IVC environments [1,2]. In vitro embryo production (IVP) procedures, including oocyte in vitro maturation (IVM) and early embryo IVC, have attracted attention due to their potential applications in agricultural production and scientific research [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

Peng

Chen

Luo

et al. 2020

Journal of Reproduction and Development

View full text Add to dashboard Cite

Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membrane potential, and embryonic development-related gene expression. The results showed that supplementation with 10 μM CA during in vitro culture significantly improved the cleavage rates, blastocyst formation rates, hatching rates, and total numbers of cells of parthenogenetically activated porcine embryos compared with no supplementation. More importantly, supplementation with CA also improved GSH levels and mitochondrial membrane potential, reduced natural ROS levels in blastomeres, upregulated Nanog , Sox2 , Gata4 , Cox2 , Itga5, and Rictor expression, and downregulated Birc5 and Caspase3 expression. These results suggest that CA can improve early porcine embryonic development by regulating oxidative stress. This study elucidates the effects of CA on early embryonic development and their potential mechanisms, and provides new applications for improving the quality of in vitro -developed embryos.

show abstract

Section: Introductionmentioning

confidence: 99%

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

Peng

Chen

Luo

et al. 2020

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

Clotting Dysfunction in Sepsis: A Role for ROS and Potential for Therapeutic Intervention

2021

View full text Add to dashboard Cite

Sepsis is regarded as one of the main causes of death among the critically ill. Pathogen infection results in a host-mediated pro-inflammatory response to fight infection; as part of this response, significant endogenous reactive oxygen (ROS) and nitrogen species (RNS) production occurs, instigated by a variety of sources, including activated inflammatory cells, such as neutrophils, platelets, and cells from the vascular endothelium. Inflammation can become an inappropriate self-sustaining and expansive process, resulting in sepsis. Patients with sepsis often exhibit loss of aspects of normal vascular homeostatic control, resulting in abnormal coagulation events and the development of disseminated intravascular coagulation. Diagnosis and treatment of sepsis remain a significant challenge for healthcare providers globally. Targeting the drivers of excessive oxidative/nitrosative stress using antioxidant treatments might be a therapeutic option. This review focuses on the association between excessive oxidative/nitrosative stress, a common feature in sepsis, and loss of homeostatic control at the level of the vasculature. The literature relating to potential antioxidants is also described.

show abstract

Exogenous Melatonin in the Culture Medium Does Not Affect the Development of In Vivo-Derived Pig Embryos but Substantially Improves the Quality of In Vitro-Produced Embryos

et al. 2022

View full text Add to dashboard Cite

Cloned and transgenic pigs are relevant human disease models and serve as potential donors for regenerative medicine and xenotransplantation. These technologies demand oocytes and embryos of good quality. However, the current protocols for in vitro production (IVP) of pig embryos give reduced blastocyst efficiency and embryo quality compared to in vivo controls. This is likely due to culture conditions jeopardizing embryonic homeostasis including the effect of reactive oxygen species (ROS) influence. In this study, the antioxidant melatonin (1 nM) in the maturation medium, fertilization medium, or both media was ineffective in enhancing fertilization or embryonic development parameters of in vitro fertilized oocytes. Supplementation of melatonin in the fertilization medium also had no effect on sperm function. In contrast, the addition of melatonin to the embryo culture medium accelerated the timing of embryonic development and increased the percentages of cleaved embryos and presumed zygotes that developed to the blastocyst stage. Furthermore, it increased the number of inner mass cells and the inner mass cell/total cell number ratio per blastocyst while increasing intracellular glutathione and reducing ROS and DNA damage levels in embryos. Contrarily, the addition of melatonin to the embryo culture medium had no evident effect on in vivo-derived embryos, including the developmental capacity and the quality of in vivo-derived 4-cell embryos or the percentage of genome-edited in vivo-derived zygotes achieving the blastocyst stage. In conclusion, exogenous melatonin in the embryo culture medium enhances the development and quality of in vitro-derived embryos but not in in vivo-derived embryos. Exogenous melatonin is thus recommended during embryo culture of oocytes matured and fertilized in vitro for improving porcine IVP efficiency.

show abstract

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on <em>In Vitro</em> Embryo Production in Mammals

Cited by 14 publications

References 71 publications

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

Clotting Dysfunction in Sepsis: A Role for ROS and Potential for Therapeutic Intervention

Exogenous Melatonin in the Culture Medium Does Not Affect the Development of In Vivo-Derived Pig Embryos but Substantially Improves the Quality of In Vitro-Produced Embryos

Contact Info

Product

Resources

About