Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

Peng, Chunyi; Chen, Chengzhen; Luo, Dan; Yu, Wenjie; Li, Sheng-Peng; Xiao, Yue; Yuan, Bao; Liang, Shuang; Yao, Xuerui; Kim, Nam‐Hyung; Jiang, Hao

doi:10.1262/jrd.2020-086

Cited by 7 publications

(6 citation statements)

References 87 publications

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“…In contrast with our initial hypothesis, blood ROM was not affected by treatment. Those results are in contrast with previous in vitro studies that observed a lower accumulation of ROS when different cell lines were treated with carnosic acid [ 79 , 80 , 81 ]. de Oliveira, et al [ 33 ] found lower ROS concentrations when neuroblastoma cells were treated with carnosic acid during induced neurotoxicity.…”

Section: Discussioncontrasting

confidence: 99%

An Exploration of the Effects of an Early Postpartum Intravenous Infusion with Carnosic Acid on Physiological Responses of Transition Dairy Cows

2021

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The objective of the present study was to evaluate the effects of an antioxidant and anti-inflammatory compound found in rosemary plants (Salvia rosmarinus) named carnosic acid during the transition period of dairy cows. From day 1 to 3 after calving, 16 multiparous Holstein cows received a daily intravenous infusion of either 500 mL of saline (NaCl 0.9%; Saline; n = 8) or carnosic acid at a rate of 0.3 mg/kg of BW supplied in 500 mL of saline (CA; n = 8). Blood samples were taken at –7, 2, 5, 7, 14, and 21 d relative to parturition, then analyzed for metabolites related to energy metabolism, muscle mass catabolism, liver function, inflammation, and oxidative stress. CA infusion tended to improve milk performance; however, DMI was unaffected by treatment. At 2 d relative to parturition, CA cows had lower blood concentrations of haptoglobin, paraoxonase, FRAP, and NO2– than saline cows. After treatment infusions, haptoglobin remained lower in CA cows than saline at 5 d relative to parturition. Our results demonstrate that carnosic acid promoted positive responses on inflammation and oxidative stress biomarkers and may promote beneficial effects on lactation performance in peripartal dairy cows.

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Section: Discussioncontrasting

confidence: 99%

An Exploration of the Effects of an Early Postpartum Intravenous Infusion with Carnosic Acid on Physiological Responses of Transition Dairy Cows

2021

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“…IVC environments for embryos suffer from higher oxygen concentrations (Guérin et al, 2001) resulting in ROS accumulation and inhibition of embryonic development (Jamil et al, 2020). Therefore, suppressing ROS accumulation using antioxidants may improve the potential of the porcine early stage embryonic development (Jiang et al, 2018; Peng et al, 2020). In this study, OA supplementation increased GSH levels and reduced ROS accumulation.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, suppressing ROS accumulation using antioxidants may improve the potential of the porcine early stage embryonic development (Jiang et al, 2018;Peng et al, 2020). In this study, OA system of the embryo (Biswas et al, 2005;Hansen & Harris, 2015) and acts as an important indicator of antioxidant capacity (Lim & Luderer, 2018).…”

Section: Oa Ameliorates Mitochondrial Function During Early-stage Por...mentioning

confidence: 92%

Oroxin A reduces oxidative stress, apoptosis, and autophagy and improves the developmental competence of porcine embryos in vitro

Liu

Wang

et al. 2022

Reprod Domestic Animals

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Oroxin A (OA) is a flavonoid isolated from Oroxylum indicum (L.) Kurz that has various biological activities, including antioxidant activities. This study aimed to examine the viability of using OA in an in vitro culture (IVC) medium for its antioxidant effects and related molecular mechanisms on porcine blastocyst development. In this study, we investigated the effects of OA on early porcine embryo development via terminal deoxynucleotidyl transferase dUTP nick-end labeling, 5-ethynyl-2′-deoxyuridine labeling, quantitative reverse transcription PCR, and immunocytochemistry. Embryos cultured in the IVC medium supplemented with 2.5 μM of OA had an increased blastocyst formation rate, total cell number, and proliferation capacity, along with a low apoptosis rate. OA supplementation decreased reactive oxygen species levels while increasing glutathione levels. OA-treated embryos exhibited an improved intracellular mitochondrial membrane potential and reduced autophagy. Moreover, levels of pluripotency-and antioxidant-related genes were upregulated, whereas those of apoptosis-and autophagy-related genes were downregulated by OA addition. In conclusion, OA improves preimplantation embryonic development by reducing oxidative stress and enhancing mitochondrial function.

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“…The mitochondrial membrane potential is an indicator of mitochondrial function. Embryos with a high mitochondria membrane potential exert positive effects on developmental potential of porcine preimplantation embryos [ 42 ]. In this study, AN supplementation improved mitochondrial function by increasing mitochondrial content and mitochondrial membrane potential in porcine IVF embryos.…”

Section: Discussionmentioning

confidence: 99%

Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

Joo

Jeong

Lee

et al. 2023

J Animal Sci Biotechnol

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Background Anethole (AN) is an organic antioxidant compound with a benzene ring and is expected to have a positive impact on early embryogenesis in mammals. However, no study has examined the effect of AN on porcine embryonic development. Therefore, we investigated the effect of AN on the development of porcine embryos and the underlying mechanism. Results We cultured porcine in vitro-fertilized embryos in medium with AN (0, 0.3, 0.5, and 1 mg/mL) for 6 d. AN at 0.5 mg/mL significantly increased the blastocyst formation rate, trophectoderm cell number, and cellular survival rate compared to the control. AN-supplemented embryos exhibited significantly lower reactive oxygen species levels and higher glutathione levels than the control. Moreover, AN significantly improved the quantity of mitochondria and mitochondrial membrane potential, and increased the lipid droplet, fatty acid, and ATP levels. Interestingly, the levels of proteins and genes related to the sonic hedgehog (SHH) signaling pathway were significantly increased by AN. Conclusions These results revealed that AN improved the developmental competence of porcine preimplantation embryos by activating SHH signaling against oxidative stress and could be used for large-scale production of high-quality porcine embryos.

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Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

Cited by 7 publications

References 87 publications

An Exploration of the Effects of an Early Postpartum Intravenous Infusion with Carnosic Acid on Physiological Responses of Transition Dairy Cows

An Exploration of the Effects of an Early Postpartum Intravenous Infusion with Carnosic Acid on Physiological Responses of Transition Dairy Cows

Oroxin A reduces oxidative stress, apoptosis, and autophagy and improves the developmental competence of porcine embryos in vitro

Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

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