Oroxin A reduces oxidative stress, apoptosis, and autophagy and improves the developmental competence of porcine embryos in vitro

Liu, Rong‐Ping; Wang, Xin‐Qin; Wang, Jing; Luo, Dan; Li, Yinghua; Jiang, Hao; Xu, Yong‐Nan; Kim, Nam-Hyung

doi:10.1111/rda.14200

Cited by 5 publications

(5 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, we believe that different effects are caused by different concentrations of 6-G. 6-G (20 µM) inhibited blastocyst formation. This is similar to the results showing that an appropriate concentration of antioxidant supplements could improve early embryo development by reducing oxidative stress, while they would inhibit early embryo development at a high concentration [ 63 , 64 , 65 ]. This may be because oxidative stress signaling is also required during early embryonic development.…”

Section: Discussionsupporting

confidence: 88%

6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress

Peng

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

6-Gingerol, the main active ingredient in ginger, exhibits a variety of biological activities, such as antioxidant, anti-inflammatory, and anticancer activities, and can affect cell development. However, the effects of 6-gingerol on mammalian reproductive processes, especially early embryonic development, are unclear. This study explored whether 6-gingerol can be used to improve the quality of in vitro-cultured porcine embryos. The results showed that 5 μM 6-gingerol significantly increased the blastocyst formation rates of porcine early embryos. 6-Gingerol attenuated intracellular reactive oxygen species accumulation and autophagy, increased intracellular glutathione levels, and increased mitochondrial activity. In addition, 6-gingerol upregulated NANOG, SRY-box transcription factor 2, cytochrome c oxidase subunit II, mechanistic target of rapamycin kinase, and RPTOR independent companion of MTOR complex 2 while downregulating Caspase 3, baculoviral IAP repeat containing 5, autophagy related 12, and Beclin 1. Most importantly, 6-gingerol significantly increased the levels of p-extracellular regulated protein kinase 1/2 while reducing the levels of p-c-Jun N-terminal kinase 1/2/3 and p-p38. These results indicate that 6-gingerol can promote the development of porcine early embryos in vitro.

show abstract

Section: Discussionsupporting

confidence: 88%

6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress

Peng

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…The addition of antioxidants at the early stage of porcine embryo development can reduce oxidative stress and improve developmental capacity. Multifarious antioxidants, such as melatonin [ 15 , 16 ], laminarin [ 17 ], imperatorin [ 18 ], oroxin A [ 19 ], and wedelolactone [ 20 ], have been reported to have antioxidant activity and can improve the early development ability of porcine embryos. However, the current IVC environment does not strongly imitate the environment in vivo.…”

Section: Introductionmentioning

confidence: 99%

Chrysoeriol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress and Autophagy

Wang

et al. 2023

Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

Chrysoeriol (CHE) is a flavonoid substance that exists in many plants. It has various physiological and pharmacological effects, including anti-inflammatory, antioxidant, anti-tumor, and protective activity, especially for the cardiovascular system and liver. Among common livestock embryos, porcine embryos are often considered high-quality objects for studying the antioxidant mechanisms of oocytes. Because porcine embryos contain high levels of lipids, they are more vulnerable to external stimuli, which affect development. Our study explored the influence of CHE supplementation on oxidative stress in porcine oocytes and its possible mechanisms. Different concentrations of CHE (0, 0.1, 1, and 3 µM) were supplemented in the in vitro culture medium of the porcine oocytes. The results showed that supplementation with 1 µM CHE significantly increased the blastocyst rate and total cell number of embryos in vitro. After finding the beneficial effects of CHE, we measured reactive oxygen species (ROS), glutathione (GSH), and mitochondrial membrane potential (MMP) when the oocytes reached the 4-cell stage of development and determined the levels of apoptosis, cell proliferation, and autophagy at the blastocyst stage of development. The expression levels of some related genes were preliminarily detected by qRT-PCR. The results showed that the apoptosis of blastocysts in the CHE-treated culture also decreased compared with the untreated culture. Furthermore, CHE downregulated intracellular ROS and increased GSH in the embryos. CHE was also shown to improve the activity of mitochondria and inhibit the occurrence of autophagy. In addition, antioxidant-related genes (SOD1, SOD2, and CAT) and cell pluripotency-related genes (SOX2, OCT4, and NANOG) were upregulated. At the same time, apoptosis-related (Caspase 3) and autophagy-related (LC3B) genes showed a downward trend after supplementation with CHE. These results indicate that CHE improved the development of porcine embryos in vitro by reducing oxidative stress and autophagy levels.

show abstract

“…Maackiain is an isoflavanone compound with antioxidant [ 53 ], anti-inflammatory [ 16 ], antibacterial [ 15 ], antitumor [ 49 ] and other pharmacological effects. Oroxin A exhibits antioxidant activities [ 25 ]. Oroxin A also has the ability to activate PPARγ and inhibit α-glucosidase and is a promising candidate for diabetes intervention [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…Other types of compounds also exhibit pharmacological activity. The amounts of abrine and hypaphorine in AmS are higher than those in AcS, which are alkaloids with various pharmacological activities, such as anti-inflammatory and antioxidant activities [ 25 , 26 ]. AmR is rich in soyasaponins, which are triterpenoids with a variety of biological activities, such as antitumor [ 43 ], obesity prevention [ 18 ] and immunomodulation activity [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics analysis reveals the differences between Abrus cantoniensis Hance and Abrus mollis Hance

Cao,

Chen,

Huang

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

Background Abrus cantoniensis Hance. (Ac) and Abrus mollis (Am), two edible and medicinal plants with economic value in southern China, belong to the Abrus genus. Due to its growth characteristics, Am often replaces Ac in folk medicine. However, the latest National Pharmacopeia of China only recommends Ac. The differences in the metabolite composition of the plants are directly related to the differences in their clinical efficacy. Results The difference in metabolites were analyzed using an untargeted metabolomic approach based on ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC‒ESI‒MS/MS). The roots (R), stems (S) and leaves (L) of the two varieties were examined, and 635 metabolites belonging to 8 classes were detected. A comparative study revealed clear variations in the metabolic profiles of the two plants, and the AmR group had more active ingredients (flavonoids and terpenoids) than the AcR group. The metabolites classified as flavonoids and triterpene saponins showed considerable variations among the various samples. Both Ac and Am had unique metabolites. Two metabolites (isovitexin-2''-xyloside and soyasaponin V) specifically belong to Ac, and nine metabolites (vitexin-2"-O-galactoside, ethyl salicylate, 6-acetamidohexanoic acid, rhein-8-O-glucoside, hederagenin-3-O-glucuronide-28-O-glucosyl(1,2)-glucoside, methyl dioxindole-3-acetate, veratric acid, isorhamnetin-3-O-sophoroside-7-O-rhamnoside, and isorhamnetin-3-O-sophoroside) specifically belong to Am. Conclusions The metabolite differences between Ac and Am cause the differences in their clinical efficacy. Our findings serve as a foundation for further investigation of biosynthesis pathways and associated bioactivities and provide guidance for the clinical application of traditional Chinese medicine.

show abstract

Oroxin A reduces oxidative stress, apoptosis, and autophagy and improves the developmental competence of porcine embryos in vitro

Cited by 5 publications

References 78 publications

6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress

6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress

Chrysoeriol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress and Autophagy

Metabolomics analysis reveals the differences between Abrus cantoniensis Hance and Abrus mollis Hance

Contact Info

Product

Resources

About