Nitric oxide levels in periodontitis with relation to obesity

Dhotre, Pradnya Shree; Bhogade, R. B.; Shaikh, Abdul Kayyum

doi:10.5958/2394-6377.2016.00010.1

Cited by 3 publications

(4 citation statements)

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“…Furthermore, many salivary microorganisms reduce nitrates (V) to nitrates (III) at acidic pH. Obesity has also turned out to have a significant effect on the increase of salivary NO levels in periodontitis [31]. A meta-analysis by Chen et al [32], involving three consistent studies (described in Table 2 [25,26,28]) and referring to 140 subjects suffering from periodontitis and 140 belonging to the control group, has shown a significant increase of NO levels in non-stimulated saliva in the course of periodontitis (p < 0.001, with a surprisingly high non-uniformity index of 93.4%).…”

Section: Discussionmentioning

confidence: 99%

Nitrosative Stress Biomarkers in the Non-Stimulated and Stimulated Saliva, as well as Gingival Crevicular Fluid of Patients with Periodontitis: Review and Clinical Study

2020

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Diagnosis of periodontopathy is complex and includes defining the cause, type, stage, and grade of periodontitis. Therefore, alternative diagnostic methods are sought to indicate the progression of inflammation or to determine the effectiveness of therapy. Gingival crevicular fluid (GCF) biomarkers can be particularly useful because they most likely reflect the disease process of the periodontal tissues. However, the difficulty of collecting GCF for testing is the reason for the limited use in diagnostics. Because periodontitis is the primary source of nitrogen free radicals in the oral cavity, the aim of the study was to evaluate the biomarkers of nitrosative stress (nitric oxide, peroxynitrite, and S-nitrosothiols) in GCF, non-stimulated and stimulated saliva of 90 patients with periodontitis. The study group was divided into two subgroups, depending on the stage of the disease severity. We showed a significantly higher concentration of all assessed biomarkers in the non-stimulated and stimulated saliva of patients with periodontitis. However, significant changes in GCF has been shown only for peroxynitrite. The studied biomarkers did not correlate with clinical periodontal status, which probably results from their short-duration activity and the impact on a few factors in the oral cavity. Saliva and gingival fluid are not very useful in the differential diagnosis of periodontitis.

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Section: Discussionmentioning

confidence: 99%

Nitrosative Stress Biomarkers in the Non-Stimulated and Stimulated Saliva, as well as Gingival Crevicular Fluid of Patients with Periodontitis: Review and Clinical Study

2020

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“…Nitric oxide levels in both serum and saliva were statistically elevated (P a1+a2 + b1 =0.001 and P b2 =0.01) in pregnant (66.82±9.3 μmol/liter (L) and 55.33±9.1 μmol/L, respectively) and nonpregnant (53.03±2.5 μmol/L and 49.93±9.5 μmol/L, respectively) women with gingivitis compared with the control group (31.64±4.4 μmol/L and 23.50±2.2 μmol/L, respectively). It has also been confirmed by reports of increased levels of nitrogen oxides in the blood in a range of diseases [24]. The most important catalyst for the NO synthesis is bacterial products, macrophages, and other inflammatory cells can stimulate their formation and release.…”

Section: Resultsmentioning

confidence: 63%

“…Thus, resulting nitrite was detected by linking sulfanilamide with naphthy-lethylenediamine to form a purplecolored compound. Color intensity was read at 540 nm with a spectrophotometer [24]. Menden method was used to measure Crp concentration in serum [25], and in saliva by Sunderman and Nomoto [26].…”

Section: Biochemical Biomarkers Detectionmentioning

confidence: 99%

Untitled

2022

J. Med. Chem. Sci.

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Background: Gingivitis is an inflammation limited to marginal gingival tissues, which can be referred to as the mildest form of periodontal diseases, initial signs of inflammation which include: redness, bleeding, swelling, exudation, and to a lesser extent pain, which indicate the presence of gingivitis. Current research was proposed to detect the correlation between pregnancy status and oxidative stress (OS) levels among gingivitis pregnant women and to elucidate the role of pregnancy status in the pathogenicity of gingivitis. Methods: The current study was conducted in the Dental Center at Thi-Qar province (Iraq), and it included three study groups: 35 pregnant women with gingivitis, 35 non-pregnant women with gingivitis, and 30 non-pregnant women without gingivitis (the healthy control), with an age balance between the three study groups. Serological and salivary levels of malondialdehyde (MDA), total anti-oxidant capacity (TAC), protein carbonyl (Pc), protein, nitric oxide (NO), and ceruloplasmin (Crp) were biochemically determined. Results:The results of the tests showed that the concentrations of MDA, Pc, the total proteins (TP), NO, and Crp were significantly higher among pregnant and non-pregnant women with gingivitis compared with healthy controls in both serum and saliva, whereas the inverse findings were reported for TCA biomarker. The same results profiles were reported in comparison between pregnant women with gingivitis and non-pregnant women with gingivitis for serum biomarkers. For saliva biomarkers, the results revealed significant elevated levels of MDA, Pc, and NO among pregnant gingivitis women in comparison with non-pregnant gingivitis, whereas the other biomarkers revealed non-significant differences. Conclusions: The pregnancy status had an effect on the OS level which lead to a higher level of OS in both serum and saliva and the correlation between the pregnancy status and serum and/or saliva OS levels was positive which indicated the possible utility of its present lead to gingivitis development.

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“…Thus, malfunctioning of the microvascular endothelium produces alterations of the microcirculatory system leading to heterogeneous capillary perfusion with a reduced capacity for tissue oxygen extraction 27 and bone regeneration. 28 …”

Section: Discussionmentioning

confidence: 99%