Nitric oxide in respiratory diseases

Nevin, Barra J.; Broadley, Kenneth J.

doi:10.1016/s0163-7258(02)00262-0

Cited by 52 publications

(42 citation statements)

References 333 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…12) Of these, iNOS is an important enzyme involved in the regulation of inflammation 13) and in the generation of a large amount of NO. 14) Overproduction of NO by iNOS has been implicated in various pathological processes including septic shock, tissue damage following inflammation, and rheumatoid arthritis. [15][16][17][18][19] Therefore, suppression of nitric oxide (NO) production in macrophages could be a target for potential anti-inflammatory drugs.…”

mentioning

confidence: 99%

Inhibition of Nitric Oxide Production in RAW 264.7 Cells by Azaphilones from Xylariaceous Fungi

Quang

Harinantenaina

Nishizawa

et al. 2006

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The Xylariaceae (Ascomycete) is a large family and currently comprises more than 36 genera. So far, at least one third of these genera have been chemically investigated. 1)One of these specific metabolites repeatedly isolated from this family is azaphilone, with various substitution groups linked to the main skeleton.2,3) Although previous publications showed that azaphilones exhibit broad activities in many biological systems, such as cholesteryl ester transfer protein, 4) monoamine oxidase inhibition, 5) endothelin receptor binding, 6) the inhibition of gp120-CD4 binding, 7) telomerase inhibition, 8) the induction of Epstein-Barr virus, 9) and antimicrobial activities, 3,10,11) there is no report about the inhibition of nitric oxide (NO) production by azaphilones.NO is synthesized from L-arginine by nitric oxide synthase (NOS). Currently, three distinct isoforms of NOS have been identified: Neuronal NOS (nNOS), inducible NOS (iNOS), and endothelial NOS (eNOS).12) Of these, iNOS is an important enzyme involved in the regulation of inflammation 13) and in the generation of a large amount of NO.14) Overproduction of NO by iNOS has been implicated in various pathological processes including septic shock, tissue damage following inflammation, and rheumatoid arthritis. [15][16][17][18][19] Therefore, suppression of nitric oxide (NO) production in macrophages could be a target for potential anti-inflammatory drugs.In the present study, we screened the inhibitory activity of fifteen azaphilones, which were isolated from mushrooms belonging to the Xylariaceae family, and also elucidated the mechanism of inhibition of NO production stimulated by lipopolysaccharide (LPS) in RAW 264.7 cells. MATERIALS AND METHODS Isolation of AzaphilonesFifteen azaphilones, specifically daldinins C, E and F (1-3) from Hypoxylon fuscum, 20,21) multiformin D (4) from H. multiforme, 11) sassafrins A-C (5-7) from Creospharia sassafras, 10) entonaemin A (8) and rubiginosins A-C (9-11) from H. rubiginosin, 22) cohaerins A and B (12, 13) from H. cohaerens, 23) rutilins A and B (14, 15) from H. rutilin, 24) were isolated from xylariaceous fungi and their structures characterized by a combination of NMR spectrometry and X-ray crystallographic analysis.Cell Culture RAW 264.7 cells, a mouse macrophage cell line, were grown in RPMI 1640 supplemented with 10% fetal bovine serum, kanamycin (50 mg/ml), and ampicillin (60 mg/ml) at 37°C in an atmosphere of 5% CO 2 and 95% air.Inhibition of NO Production of Fifteen Azaphilones One hundred microliters of RAW 264.7 cells (8ϫ10 5 cells/ ml) was pipetted into the wells of 96-well culture plates and then cultured. After 24 h incubation, 50 ml of medium containing various concentrations of each azaphilone derivative diluted in DMSO was added to each well. The final DMSO concentration was less than 0.1%. At this concentration, DMSO did not show any NO-induction without stimulation with LPS. Then, 50 ml of vehicle or LPS purified from Pantoea agglomerans (4 mg/ml) was added to each well. The cells were further in...

show abstract

mentioning

confidence: 99%

Inhibition of Nitric Oxide Production in RAW 264.7 Cells by Azaphilones from Xylariaceous Fungi

Quang

Harinantenaina

Nishizawa

et al. 2006

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…As observed previously [10], NO and CO, both having cGMP as a second messenger, participate as neurotransmitters in sub-maximal NANC tracheal relaxations, as shown by the inhibition caused by L -NAME and ZnPP-IX, respectively. Among the NO synthase inhibitors, L -NAME is one of the most widely used compounds to assess the nitrergic component of the NANC inhibitory response [30]. ZnPP-IX is a haem oxygenase-2 inhibitor that is used to assess the involvement of CO in functional studies.…”

Section: Discussionmentioning

confidence: 99%

Inhaled Ammonium Persulphate Inhibits Non-Adrenergic, Non-Cholinergic Relaxations in the Guinea Pig Isolated Trachea

et al. 2009

View full text Add to dashboard Cite

Background: Persulphates can act both as irritants and sensitizers in inducing occupational asthma. A dysfunction of nervous control regulating the airway tone has been hypothesized as a mechanism underlying bronchoconstriction in asthma. Objectives: It was the aim of this study to investigate whether inhaled ammonium persulphate affects the non-adrenergic, non-cholinergic (NANC) inhibitory innervation, the cholinergic nerve-mediated contraction or the muscular response to the spasmogens, carbachol or histamine, in the guinea pig epithelium-free, isolated trachea. Methods: Male guinea pigs inhaled aerosols containing ammonium persulphate (10 mg/m³ for 30 min for 5 days during 3 weeks). Control animals inhaled saline aerosol. NANC relaxations to electrical field stimulation at 3 Hz were evaluated in whole tracheal segments as intraluminal pressure changes. Drugs inactivating peptide transmission, nitric oxide synthase, carbon monoxide production by haem oxygenase-2 and soluble guanylyl cyclase were used to assess the involvement of various inhibitory neurotransmitters. Carbachol and histamine cumulative concentration-response curves were obtained. Results: In both groups, nitric oxide and carbon monoxide participated to the same extent as inhibitory neurotransmitters. In exposed animals, the tracheal NANC relaxations were reduced to 45.9 ± 12.1% (p < 0.01). The cholinergic nerve-mediated contractions to electrical field stimulation and the muscular response to histamine were not modified by ammonium persulphate exposure. The muscular response to carbachol was unaffected up to 1 µM. Conversely, the response to the maximal concentration of carbachol (3 µM) was increased (p < 0.01). Conclusion: Ammonium persulphate inhalation at high concentrations impairs the nervous NANC inhibitory control in the guinea pig airways. This may represent a novel mechanism contributing to persulphate-induced asthma.

show abstract

“…NO is also a good marker for monitoring respiratory diseases [7]. One of the methods recently investigated is the determination of NO in the exhaled breath condensate (EBC), which, except NO, contains several important endogenous substances; such as hydrogen peroxide, thiobarbituric acid-relevant substances, isoprostanes, prostaglandins etc.…”

Section: Introductionmentioning

confidence: 99%

Nickel Hexacyanoferrate‐Based Sensor Electrode for the Detection of Nitric Oxide at Low Potentials

Krylov

Lisdat

2007

Electroanalysis

View full text Add to dashboard Cite

Recently transition metal hexacyanoferrates, analogues of Prussian Blue, have found application in electroanalysis for the detection of biologically relevant species. Our study describes the development of a novel electrode based on nickel hexacyanoferrate (NiHCF) for the sensorial NO determination. A NiHCF layer was deposited on platinum by cyclic voltammetry in a solution of nickel (II) chloride and potassium hexacyanoferrate (III). The electrode was found to be active for NO reduction. The interaction with the radical was studied voltammetrically within the range from 0 V up to þ 0.4 V vs. Ag/AgCl/1 M KCl. The most appropriate potential for an amperometric detection was determined to be þ 0.25 V due to the advantageous signal/noise ratio. The sensitivity of the electrodes was found to be 2.0 -2.3 A M À1 cm À2. The sensor response of the most important interferents for NO analysis, hydrogen peroxide, ascorbic acid and nitrite, was measured and determined to be sufficiently low.

show abstract

Nitric oxide in respiratory diseases

Cited by 52 publications

References 333 publications

Inhibition of Nitric Oxide Production in RAW 264.7 Cells by Azaphilones from Xylariaceous Fungi

Inhibition of Nitric Oxide Production in RAW 264.7 Cells by Azaphilones from Xylariaceous Fungi

Inhaled Ammonium Persulphate Inhibits Non-Adrenergic, Non-Cholinergic Relaxations in the Guinea Pig Isolated Trachea

Nickel Hexacyanoferrate‐Based Sensor Electrode for the Detection of Nitric Oxide at Low Potentials

Contact Info

Product

Resources

About