Nitric Oxide in Occurrence, Progress and Therapy of Lung Cancer: A Systemic Review and Meta-analysis

Zhou, Hongbin; Chen, Zhewen; Chen, Ying; Li, Jiuke; Ye, Sa

doi:10.21203/rs.3.rs-249109/v1

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…NO is a small gas molecule widely distributed in the heart, lung, liver, kidney and other organs (Chong et al, 2014; Kandil et al, 2021; Ozdemir et al, 2021; Zhou et al, 2021). It can be freely diffused through the cell membrane and is an important intercellular messenger substance.…”

Section: Discussionmentioning

confidence: 99%

Parecoxib ameliorates renal toxicity and injury in sepsis‐induced mouse model and LPS ‐induced HK ‐2 cells

Guo

Chen

et al. 2021

Drug Dev Res

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Parecoxib is a selective COX‐2‐specific inhibitor, which has been demonstrated to inhibit sepsis‐induced systemic inflammation, but its role in sepsis‐induced acute kidney injury has not been studied. This study was designed to investigate the effects of Parecoxib on sepsis‐induced acute kidney injury. In this study, the mice sepsis model was established using an internationally recognized cecal ligation and puncture (CLP). Hematoxylin–eosin staining was performed to examine kidney injury. Biochemical kit was used to detect the expression of BUN and Cre in serum, and ELISA was used to detect the expression of inflammatory factors in renal tissue. Tunel staining was used to detect tissue apoptosis. Furthermore, CCK‐8 assay was used to detect the cell viability of HK‐2 cells and RT‐qPCR was used to detect the expression of LPS‐induced inflammatory factors in HK‐2 cells.TUNEL staining was used to detect the level of cell apoptosis. Finally, the expressions of COX‐2, p‐NF‐kB P65, p‐IKKβ, NF‐kB P65, IKKβ, Kim1, NGAL, iNOS, VEGF, VEGFR2, CD31 and apoptosis‐related proteins in renal tissues and HK‐2 cells were detected by Western blot. We discovered that parecoxib could alleviate renal pathological changes, reduce renal function injury, and inhibit renal pathology to inhibit the release of inflammatory factors in renal tissue. Parecoxib inhibited sepsis induced microvascular damage and apoptosis in renal tissue. Parecoxib reduced the inflammation and apoptosis of renal tubular epithelial cells induced by LPS. Our data suggest that Parecoxib ameliorates sepsis‐induced kidney injury, and may have potential as a novel therapeutic method for treating sepsis‐induced kidney injury.

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