Nitric oxide has a regulatory effect in the germination of conidia of Colletotrichum coccodes

Wang, Jennifer; Higgins, Verna J.

doi:10.1016/j.fgb.2004.12.006

Cited by 91 publications

(85 citation statements)

References 33 publications

(36 reference statements)

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“…Nitric oxide has been detected in many fungi, including C. minitans (15,19,20,29,30), but its origin in fungi is still unknown. In animals the biosynthesis of NO is primarily catalyzed by the enzyme nitric oxide synthase (18), and in plants the production of NO is catalyzed by nitrate reductase, xanthine oxidoreductase, certain plasma membrane-bound enzymes, and NOS-like enzymes (5).…”

Section: Discussionmentioning

confidence: 99%

“…NO stimulates the formation of sexual fruiting bodies in the basidiomycete Flammulina velutipes (26). It is also involved in other fungal physiological processes, such as suppression of pseudomycelial formation in the yeast Candida tropicalis (35), and delay in conidial germination in Colletotrichum coccodes (30). In addition, NO formation was detected in the mycobiont of the lichen Ramalina lacera during transitions between desiccation and rehydration (31).…”

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confidence: 99%

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Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Fù

Xiè

et al. 2010

Appl Environ Microbiol

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Understanding signaling pathways that modulate conidiation of mitosporic fungi is of both practical and theoretical importance. The enzymatic origin of nitric oxide (NO) and its roles in conidiation by the sclerotial parasite Coniothyrium minitans were investigated. The activity of a nitric oxide synthase-like (NOS-like) enzyme was detected in C. minitans as evidenced by the conversion of L-arginine to L-citrulline. Guanylate cyclase (GC) activity was also detected indirectly in C. minitans with the GC-specific inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), which significantly reduced production of cyclic GMP (cGMP). The dynamics of NOS activity were closely mirrored by the cGMP levels during pycnidial development, with the highest levels of both occurring at the pycnidial initiation stage of C. minitans. Furthermore, the NO donor, sodium nitroprusside (SNP), stimulated the accumulation of cGMP almost instantly in mycelium during the hyphal growth stage. When the activity of NOS or GC was inhibited with N-nitro-L-arginine or ODQ, conidial production of C. minitans was suppressed or completely eliminated; however, the suppression of conidiation by ODQ could be reversed by exogenous cGMP. The results also showed that conidiation of an L-arginine auxotroph could be restored by the NO donor SNP, but not by cGMP. Thus, NO-mediated conidiation has more than one signal pathway, including the cGMP signal pathway and another yet-unknown pathway, and both are essential for conidiation in C. minitans.Coniothyrium minitans is a sclerotial parasite of the notorious plant pathogen Sclerotinia sclerotiorum, and its potential for biological control of Sclerotinia diseases has been well demonstrated in several countries (13,17,21,28,33,34,36,37). Efficient production of conidia will further enhance the potential of C. minitans as a biological control agent. Understanding signaling pathways that modulate conidiation of C. minitans will not only facilitate manipulation of the biocontrol agent for commercial use but also advance our understanding of fungal biology.Nitric oxide (NO) is a widespread signaling molecule involved in regulation of a wide range of cellular functions in animals and plants (7). NO synthesis and signaling have been well studied in animals and plants. In mammals, NO plays roles in relaxation of smooth muscle, inhibition of platelet aggregation, neural communication and immune regulation, while in plants NO is involved in disease resistance, abiotic stress, cell death, respiration, senescence, root development, seed germination, and other functions (reviewed in references 6 and 32). NO is also involved in the development of several members of the mycetozoa, such as, Dictyostelium discoideum (10) and Physarum polycephalum (27). The wide variety of effects reflects the basic signaling mechanism that is used by mammals, plants, and virtually all organisms (2). Despite of the extensive research on NO synthesis and signaling processes in animals and plants, our knowledge about NO in fungi is very limi...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Fù

Xiè

et al. 2010

Appl Environ Microbiol

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“…Yellow, water-soluble NBT is reduced by superoxide radicals to blue, water-insoluble formazan (Grosskinsky et al, 2012;Jabs et al, 1996;Kawarazaki et al, 2013;L'Haridon et al, 2011;Liao et al, 2012;Wang and Higgins, 2006;Xia et al, 2009). Superoxide anion radicals can also be detected using dihydroethidium (DHE), a cell-permeable blue fluorescent stain that forms red fluorescent oxyethidium upon oxidation and intercalates with nucleic acids (see recent applications in Lehotai et al, 2011;Mai et al, 2013;Pet} o et al, 2013).…”

Section: Detection Of Rosmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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“…It is also shown that NOS-dependent NO production is associated with salt tolerance (Zhao et al, 2004), high light stress (Xu et al, 2009) and P deficiency (Wang et al, 2010). In fungi, it is only indicated by NOS inhibitors that NOS-like protein-associated NO production plays a role in development of sporangiophores (Maier et al, 2001), ascospore (Kig and Temizkan, 2009), zoospore (Vieira et al, 2009), photo-conidiation of Neurospora crassa (Ninnemann and Maier, 1996;Gong et al, 2007;Li et al, 2010), and the conidial germination of Colletotrichum coccodes (Wang and Higgins, 2005). These results suggest that different NO sources are coupled to different physiological and developmental processes, as well as various abiotic stresses.…”

Section: Heat Stress Induced An Increase In Endogenous No Levelmentioning

confidence: 99%

“…Also, low levels of NO show a cytoprotective effect on S. cerevisiae during heat shock stress and high hydrostatic pressure (Domitrovic et al, 2003). NO is also involved in the process of development such as conidiation (Ninnemann and Maier, 1996;Wang and Higgins, 2005;Gong et al, 2007;Li et al, 2010), sporangiophores (Maier et al, 2001), ascospore (Kig and Temizkan, 2009), and zoospore (Vieira et al, 2009). These results suggest that NO may also regulate stress responses and developmental processes in fungi.…”

Section: Introductionmentioning

confidence: 97%

Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis

WeiWei

Huang

Chen

et al. 2012

Fungal Genetics and Biology

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Nitric oxide has a regulatory effect in the germination of conidia of Colletotrichum coccodes

Cited by 91 publications

References 33 publications

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Reactive oxygen species and plant resistance to fungal pathogens

Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis

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