Nitric oxide and tissue destruction

Kendall, H. K.; Marshall, R. I.; Bartold, P. Mark

doi:10.1034/j.1601-0825.2001.70102.x

Cited by 117 publications

(108 citation statements)

References 125 publications

(130 reference statements)

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“…In contrast, one report has described a reduction of salivary NO levels in individuals with adult periodontitis and with aggressive periodontitis (29). This report is in disagreement with the vast majority of the literature that describes increased levels of NO in periodontal disease (5,(30)(31)(32).…”

Section: Discussioncontrasting

confidence: 55%

Nitric oxide levels in saliva increase with severity of chronic periodontitis

et al. 2007

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The aims of this study were to compare nitric oxide (NO) levels in stimulated whole saliva from individuals with and without generalized chronic periodontitis (GCP), and to evaluate correlations between these levels with a clinical diagnostic parameter. According to specific criteria, 30 individuals were divided into three groups: one comprising individuals without periodontitis (GC), a second comprising individuals with moderate GCP (GM), and a third comprising individuals with advanced GCP (GA). Samples were collected and NO levels measured. NO in the GCP group (GM: 7.78 µM; GA: 15.79 µM) was higher than in the GC group (5.86 µM). NO levels in the GA group were significantly higher (P < 0.0001) than in the GC group, and could also differentiate (P < 0.0001) the moderate and advanced forms of the disease.

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Section: Discussioncontrasting

confidence: 55%

Nitric oxide levels in saliva increase with severity of chronic periodontitis

et al. 2007

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“…It is hypothesized that the inhibition of activity could be due to the NOmediated nitrosylation of receptors or other proteins involved in the secretion signal transduction pathways (Caulfield et al 2009). However, this relationship between increased nitrite concentrations and salivary gland hypofunction is more complicated, since other oral inflammatory disorders exhibit increased nitrite concentrations in saliva as well (Kendall et al 2000, ;Kendall, Marshall, and Bartold 2001, ;Ohashi, Iwase, and Nagumo 1999).…”

Section: Potential Roles Of No and Inos In Exocrine Hypofunctionmentioning

confidence: 99%

Mechanisms of Salivary Gland Secretory Dysfunction in Sjögren’s Syndrome

Pauley¹,

Lee²,

Gauna³

et al. 2012

Insights and Perspectives in Rheumatology

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“…In high concentrations, NO can cause severe tissue damage (12). The quantification of NO produced by cells in culture was determined by detection of nitrite accumulation in the cell culture supernatant by the diazotisation reaction with Griess reagent (Sigma-Aldrich).…”

Section: Nitric Oxide Production (No)mentioning

confidence: 99%

“…Cell viability was determined by means of the methyl thiazolyl tetrazolium (MTT) colorimetric assay (12) This method determines the respiratory activity of the cell culture by cleavage of MTT salt [3-(4,5-dimethyltriazol-2YL) -2,5-diphenyl bromide tetrazolium (Sigma-Aldrich, St. Louis, MO, USA), by the succinate dehydrogenase enzyme system, resulting in soluble crystals (formazan crystals).…”

Section: Cell Viability Evaluation (Mtt Assay)mentioning

confidence: 99%

Functional Differences In Gingival Fibroblasts Obtained from Young and Elderly Individuals

et al. 2016

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Fibroblasts participate in the wound repair process through proliferation and migration as well as the synthesis of factors growth and extracellular matrix molecules. However, cell aging and the individual himself can lead to reduction of cell functions and consequently, the ability of tissue repair. This study evaluated the activity of gingival fibroblasts from young (Y) and elderly (Y) patients and their responsiveness to tumor necrosis factor alpha (TNF-α). Gingival fibroblasts were isolated from six patients (3Y; and 3E) and seeded in complete culture medium (DMEM). For cell viability analysis, total protein production and collagen synthesis, fibroblasts were cultured in 96-well plates for 24, 48 or 72 h (n=36). Cell responses to TNF-α, was evaluated by application of this cytokine to cultured cells (100 ng/mL) for 24 h, followed by evaluation of reactive oxygen species (ROS), nitric oxide (NO) and CCL5 production (n=36). Data were analyzed by Kruskal-Wallis and the MannWhitney U tests (α = 0.05). Viability of E fibroblasts was higher than Y fibroblasts for 24 and 48 h, but these cells showed gradual reduction of viability over the course of time. For Y cells, reduced collagen synthesis was observed at 48 h. No difference was observed in ROS production for both cells after TNF-α exposure. However, both cultures showed increased production of NO and CCL5 in the presence of TNF-α. Functional differences and distinct responsiveness to TNF-α were observed according to patient's age. Functional Differences In Gingival F i b r o b l a s t s O b t a i n e d f r o m Young and Elderly Individuals

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Nitric oxide and tissue destruction

Cited by 117 publications

References 125 publications

Nitric oxide levels in saliva increase with severity of chronic periodontitis

Nitric oxide levels in saliva increase with severity of chronic periodontitis

Mechanisms of Salivary Gland Secretory Dysfunction in Sjögren’s Syndrome

Functional Differences In Gingival Fibroblasts Obtained from Young and Elderly Individuals

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