Functional Differences In Gingival Fibroblasts Obtained from Young and Elderly Individuals

Abstract

Fibroblasts participate in the wound repair process through proliferation and migration as well as the synthesis of factors growth and extracellular matrix molecules. However, cell aging and the individual himself can lead to reduction of cell functions and consequently, the ability of tissue repair. This study evaluated the activity of gingival fibroblasts from young (Y) and elderly (Y) patients and their responsiveness to tumor necrosis factor alpha (TNF-α). Gingival fibroblasts were isolated from six patients (3Y; and 3E) and seeded in complete culture medium (DMEM). For cell viability analysis, total protein production and collagen synthesis, fibroblasts were cultured in 96-well plates for 24, 48 or 72 h (n=36). Cell responses to TNF-α, was evaluated by application of this cytokine to cultured cells (100 ng/mL) for 24 h, followed by evaluation of reactive oxygen species (ROS), nitric oxide (NO) and CCL5 production (n=36). Data were analyzed by Kruskal-Wallis and the MannWhitney U tests (α = 0.05). Viability of E fibroblasts was higher than Y fibroblasts for 24 and 48 h, but these cells showed gradual reduction of viability over the course of time. For Y cells, reduced collagen synthesis was observed at 48 h. No difference was observed in ROS production for both cells after TNF-α exposure. However, both cultures showed increased production of NO and CCL5 in the presence of TNF-α. Functional differences and distinct responsiveness to TNF-α were observed according to patient's age. Functional Differences In Gingival F i b r o b l a s t s O b t a i n e d f r o m Young and Elderly Individuals

Mouthwash with Active Oxygen (blue®m) Reduces Postoperative Inflammation and Pain

“…However, there was no difference when analyzing the number of more healed sites (pink pixels). The work of Pansani et al [ 34 ] clarifies this issue. It has been reported that the individual's age factor alters the synthesis and proliferation of fibroblasts but has no influence on their inflammatory activity.…”

Uso De Fatores De Crescimento Na Prevenção De Complicações Pós-Operatória De Cirurgias Plásticas – Uma Revisão Para Um Capítulo

“…17 Os fatores de crescimento possuem ação entre as junções da epiderme, derme e hipoderme, onde eles penetram nestes tecidos e enviam sinais entre as células em que tiveram contato, provocando assim um efeito cascata em que são sintetizados vários produtos como colágeno, elastina e enzimas, além de desencadear reprodução celular saudável, acelerando a cicatrização das lesões. 18,19 Ocorrendo cada vez mais o interesse e a procura de métodos e tratamentos eficazes que previnem e atenuem o envelhecimento cutâneo e das estruturas adjacentes, objetivamos identificar a real definição dos fatores de crescimento e a sua aplicabilidade. Além disso, corroborar través dos achados com a conduta médica nos protocolos, e nas limitações fisiológicas, regenerativas e cicatriciais nos pacientes pós-operatórios de cirurgias plásticas.…”