Nitrate removal characteristics and 13C metabolic pathways of aerobic denitrifying bacterium Paracoccus denitrificans Z195

Zhang, Haihan; Li, Sulin; Ma, Ben; Huang, Tinglin; Qiu, Hui; Zhao, Zhenfang; Huang, Xin; Liu, Kaiwen

doi:10.1016/j.biortech.2020.123230

Cited by 67 publications

(11 citation statements)

References 48 publications

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“…NO − 2 − N accumulation may be related to the enzyme activity of NR and NiR (shown in Figure 9). In addition, the NO − 2 − N accumulation has also been observed in some researches when nitrate was used as the sole nitrogen source (Zhang et al, 2018(Zhang et al, , 2020.…”

Section: Effects Of Sdbs and Sds Onno −mentioning

confidence: 77%

Effects of exposure to anionic surfactants (SDBS and SDS) on nitrogen removal of aerobic denitrifier

Yin

Zhang

et al. 2020

Water Environment Research

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In order to explain the effect of anionic surfactants on aerobic denitrification in the urban river, sodium dodecyl benzene sulfonate (SDBS) and sodium dodecyl sulfonate (SDS) were added in aerobic denitrifier and the efficiency of nitrogen removal, microbial mechanisms, and enzyme activity was investigated in this study. The results showed that the total nitrogen (TN) and the nitrate nitrogen (NO − 3 − N) removal efficiency decreased as an increase of SDBS concentration. In contrast, 59.70% of the TN and 75.12% of NO − 3 − N were removed as the SDBS was 0 mg/L (Control). When SDBS was 200 mg/L (SDBS-200), the removal efficiency of TN and NO − 3 − N was reduced to 4.92% and 4.00%, respectively. However, the denitrification efficiency was significantly accelerated when the concentration of SDS increased, except for 200 mg/L treatment (SDS-200). As the SDS increased from 0 to 100 mg/L (SDS-100), the removal efficiency of TN and NO − 3 − N raised from 59.70% to 70.8% and from 75.12% to 85.08%, respectively. The community structure of aerobic denitrifiers was significantly affected in the SDBS and SDS. While the Cupriavidus and Achromobacter were dominant genera in the group of Control (39.59%, and 42.45%) and SDS-100 (44.40% and 34.86%), the relative abundance of Cupriavidus increased to 84.06% and 59.45% in the group of SDBS-200 and SDS-200, respectively. Enzyme activity assays proved that the nitrite reductase (NiR) relative activity of aerobic denitrification was suppressed by both SDBS and SDS. The increase in the SDS concentrations (from 0 to 50 mg/L) resulted in sharp growth of the nitrate reductase (NR) relative activities (from 100% to 146.86%). These findings demonstrated that SDBS and SDS affected aerobic denitrification efficiency of the aerobic denitrifiers by changing its microbial community structure and enzyme activity. © 2020 Water Environment Federation • Practitioner points • SDS strengthened aerobic denitrification at low concentration, but the aerobic denitrifiers were inhibited in SDBS. • The variation of community structure played a vital role in the aerobic denitrification system. • The enzyme activity was seriously affected by SDBS and SDS. • Microorganisms and enzyme activity were synergistically involved in the aerobic denitrification.

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Section: Effects Of Sdbs and Sds Onno −mentioning

confidence: 77%

Effects of exposure to anionic surfactants (SDBS and SDS) on nitrogen removal of aerobic denitrifier

Yin

Zhang

et al. 2020

Water Environment Research

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“…When the NO 3 − -N and NO 2 − -N were separately used as the sole nitrogen, the nitrogen loss efficiency reached 26.71 ± 0.36% and 23.72 ± 0.88%, respectively. The nitrogen loss efficiency was conspicuously higher than that of 14.88% by Paracoccus denitrificans Z195 (Zhang et al, 2020) and 19.63% by Stenotrophomonas maltophilia DQ01 (Jia et al, 2019) when they were processed with nitrate alone. The NO 2 − -N accumulation was detected during NO 2 − -N transformation process.…”

Section: Nitrogen Balance Analysis In Process Of Heterotrophic Nitrif...mentioning

confidence: 79%

The Alkali-Tolerant Bacterium of Bacillus thuringiensis EM-A1 Can Effectively Perform Heterotrophic Nitrification and Aerobic Denitrification

Wang

Chen

Liang

et al. 2022

Front. Environ. Sci.

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Removal of nitrogen from hydroxylamine could effectively improve the wastewater treatment efficiency. In this work, Bacillus thuringiensis EM-A1 was obtained from a biogas digester with hydroxylamine as the only nitrogen source. Hydroxylamine (100%) and total nitrogen (71.86%) were efficiently removed under the following conditions: 30°C, sucrose as carbon source, carbon to nitrogen ratio 40, rotation speed 0 rpm, pH 9.58, and inoculant concentration of 0.58×108 colony-forming units. Ammonium was completely consumed by strain EM-A1, and 8.32 ± 0.08 mg/L of nitrate was produced during the ammonium removal process. During aerobic denitrification, the removal efficiencies of NO2−-N and NO3−-N by strain EM-A1 were 100 and 76.67%, respectively. There were about 29.34 ± 0.18%, 26.71 ± 0.36%, and 23.72 ± 0.88% initial total nitrogen lost as nitrogenous gas when NH4+, NO3−, and NO2− were separately used as the sole nitrogen source. The specific activities of ammonia monooxygenase, hydroxylamine oxidoreductase, nitrate reductase, and nitrite oxidoreductase were successfully detected as 0.37, 0.88, 0.45, and 0.70 U/mg protein, respectively. These results indicated that B. thuringiensis EM-A1 is a promising candidate for bioremediation of inorganic nitrogen from wastewater.

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“…PYX97 and Streptomyces sp. TSJ96 was examined by exploring their carbon source metabolism activity and cell metabolic characteristics, including adenosine triphosphate (ATP) and electron-transmission system activity (ETSA). , ATP and ETSA concentrations were measured at different time intervals (12, 36, and 72 h), and the method used for analysis was described in a previous study. , Moreover, the carbon source metabolism performance of monocultures and cocultures of strains PYX97 and TSJ96 was explored using Biolog-ECO technology. , After culturing for 57 h, the suspension culture liquid from each of the three cultivations was collected. The OD 600 of these suspension solutions was adjusted to 0.04.…”

Section: Methodsmentioning

confidence: 99%

Aerobic Denitrification Promoting by Actinomycetes Coculture: Investigating Performance, Carbon Source Metabolic Characteristic, and Raw Water Restoration

Ma,

Yang,

et al. 2023

Environ. Sci. Technol.

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The coculture theory that promotes denitrification relies on effectively utilizing the resources of low-efficiency denitrification microbes. Here, the strains Streptomyces sp. PYX97 and Streptomyces sp. TSJ96 were isolated and showed lower denitrification capacity when cultured individually. However, the coculture of strains PYX97 and TSJ96 enhanced nitrogen removal (removed 96.40% of total nitrogen) and organic carbon reduction (removed 92.13% of dissolved organic carbon) under aerobic conditions. Nitrogen balance analysis indicated that coculturing enhanced the efficiency of nitrate converted into gaseous nitrogen reaching 70.42%. Meanwhile, the coculturing promoted the cell metabolism capacity and carbon source metabolic activity. The coculture strains PYX97 and TSJ96 thrived in conditions of C/N = 10, alkalescence, and 150 rpm shaking speed. The coculturing reduced total nitrogen and COD Mn in the raw water treatment by 83.32 and 84.21%, respectively. During this treatment, the cell metabolic activity and cell density increased in the coculture strains PYX97 and TSJ96 reactor. Moreover, the coculture strains could utilize aromatic protein and soluble microbial products during aerobic denitrification processes in raw water treatment. This study suggests that coculturing inefficient actinomycete strains could be a promising approach for treating polluted water bodies.

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Nitrate removal characteristics and 13C metabolic pathways of aerobic denitrifying bacterium Paracoccus denitrificans Z195

Cited by 67 publications

References 48 publications

Effects of exposure to anionic surfactants (SDBS and SDS) on nitrogen removal of aerobic denitrifier

Effects of exposure to anionic surfactants (SDBS and SDS) on nitrogen removal of aerobic denitrifier

The Alkali-Tolerant Bacterium of Bacillus thuringiensis EM-A1 Can Effectively Perform Heterotrophic Nitrification and Aerobic Denitrification

Aerobic Denitrification Promoting by Actinomycetes Coculture: Investigating Performance, Carbon Source Metabolic Characteristic, and Raw Water Restoration

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