Nitrate reductase activity in nodules of pea inoculated with hydrogenase positive and hydrogenase negative strains of <i>Rhizobium leguminosarum</i>

Ligero, F.; Lluch, Carmen; Olivares, J.; Bedmar, Eulogio J.

doi:10.1111/j.1399-3054.1987.tb04293.x

Cited by 11 publications

(5 citation statements)

References 31 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strong influenee (80% effect) of the plant genotype upon the manifestation of NRA in roots and nodules and also in shoots (data not shown) of Vicia faba supports previous reports on inter-and intraspecific variability in nitrogen use between legumes (Becana and Sprent 1987, Ligero et al 1987b, Peoples et al 1987.…”

Section: Discussionsupporting

confidence: 89%

“…Nodule and root NRA was measured both in vivo and in vitro. The in vivo method was based on that described by Crafts-Brandner and Harper (1982) and Ligero et al (1987a). Subsamples of 0.25 g (whole nodules) and 0.5 g (2 to 3 mm root pieces) were placed in vials containing 5 ml of ice-cold incubation medium consisting of 0.1 M K-phosphate buffer (pH 7.0), 50 mM KNO3, 1 mM Na^EDTA, 2% (v/v) M-propanol and 0.01% (v/v) Triton X-100 as wetting agent.…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…, Many legumes derive the nitrogen needed for growth Ligero et al 1987a,b, Giannakis et al 1988), but the either by reducing Nj in their root nodules or by assitni-significance of this capacity is still unclear. The inhiblating nitrate from the growth tnedium.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nitrate metabolism in roots and nodules of Vicia faba in response to exogenous nitrate

Caba¹,

Lluch²,

Hervas³

et al. 1990

Physiol Plant

Self Cite

View full text Add to dashboard Cite

1990. Nitrate metabolism in roots and nodules of Vicia faba in response to exogenous nitrate. -Physiol. Plant. 79: 531-..539.Activities of nitrate reduction enzymes, nitrate reductase activity (NRA) and nitrite reductase activity (NiRA) from roots and nodules of 5 mutant genotypes and one commercial cultivar (Alameda) of faba bean {Vicia faba L. var. minor) grown in the presence of N; alone or with additional NOj in the medium have been studied. A naturally occurring mutant (VFM109) with impaired ability to reduce nitrate in its nodules is described. All the other cultivars of V. faba showed nodule NRA, although the range was very wide, from almost negligible (VFM72) up to 2 fimol h~' (g FW)''. This activity was entirely of plant origin. Root NRA also ranged widely accross cultivars. However, the level of activity expressed as well as the response of NRA to nitrate followed a pattern opposite to that observed in nodules. Roots and nodules of all cultivars showed very high rates of NiRA, respectively 50 and 150-fold higher than NRA, thus precluding accumulation of nitrite in these tissues. Root enzymes were significantly stimulated by nitrate while negative (NRA) or little effect (NiRA) was found for nodules. Nitrate and nitrite reduction are carried out by inducible enzymes in roots of V. faba and by constitutive enzymes in nodules, indicating that there may be different forms of these enzymes in each tissue. Differences in the plant genotype were a major cause of the variability in nitrate and nitrite reduction by nodulated root systems of V. faba.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Enzyme Assaysmentioning

confidence: 99%

See 1 more Smart Citation

Nitrate metabolism in roots and nodules of Vicia faba in response to exogenous nitrate

Caba¹,

Lluch²,

Hervas³

et al. 1990

Physiol Plant

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent reports indicate that free-living rhizobia can reduce NOj simultaneously to NHJ (assimilation) and to N, (denitrifieation) (Vairinhos et al 1986). Ligero et al (1987) have observed that pea nodules with bacteroids expressing uptake hydrogenase activity (phenotypes Hup*) show lower NRAs in vivo than nodules containing Hup" bacteroids. They suggest that the presence of higher NRAs can counteract the lack of an hydrogenase system.…”

Section: Assimilatory Nitrate and Nitrite Reductionmentioning

confidence: 99%

Nitrogen fixation and nitrate reduction in the root nodules of legumes

Becana

Sprent

1987

Physiologia Plantarum

View full text Add to dashboard Cite

Published data on, and hypotheses regarding the effect of NO−3 on functioning of legume root nodules are reviewed. It is concluded that a short‐term reversible effect of NO−3 may act via an increased resistance to O2 diffusion in nodules; this is coupled to decreased bacteroid respiration. For longer exposures to NO−3 nodule activity is irreversibly lost, but how this relates to carbohydrate deprivation or NO‐2 accumulation is unclear. Complicating factors include denitrification reactions and the interaction of NO−2 with leghaemoglobin.

show abstract

“…Nitrate reductase (NR) activity was determined in leaves by the modified in vivo method (Jaworski, 1971;Ligero et al, 1987;Karwat et al, 2019). The NR activity was determined in the flag leaf, which was harvested between Z51 and Z53 stages (Zadoks et al, 1974).…”

Section: Plant Determinations and Enzymatic Activitymentioning

confidence: 99%

Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties

et al. 2022

View full text Add to dashboard Cite

Synthetic nitrification inhibitors (SNI) and biological nitrification inhibitors (BNI) are promising tools to limit nitrogen (N) pollution derived from agriculture. Modern wheat cultivars lack sufficient capacity to exude BNIs, but, fortunately, the chromosome region (Lr#n-SA) controlling BNI production in Leymus racemosus, a wild relative of wheat, was introduced into two elite wheat cultivars, ROELFS and MUNAL. Using BNI-isogenic-lines could become a cost-effective, farmer-friendly, and globally scalable technology that incentivizes more sustainable and environmentally friendly agronomic practices. We studied how BNI-trait improves N-uptake, and N-use, both with ammonium and nitrate fertilization, analysing representative indicators of soil nitrification inhibition, and plant metabolism. Synthesizing BNI molecules did not mean a metabolic cost since Control and BNI-isogenic-lines from ROELFS and MUNAL presented similar agronomic performance and plant development. In the soil, ROELFS-BNI and MUNAL-BNI plants decreased ammonia-oxidizing bacteria (AOB) abundance by 60% and 45% respectively, delaying ammonium oxidation without reducing the total abundance of bacteria or archaea. Interestingly, BNI-trait presented a synergistic effect with SNIs since made it also possible to decrease the AOA abundance. ROELFS-BNI and MUNAL-BNI plants showed a reduced leaf nitrate reductase (NR) activity as a consequence of lower soil NO3− formation and a higher amino acid content compared to BNI-trait lacking lines, indicating that the transfer of Lr#-SA was able to induce a higher capacity to assimilate ammonium. Moreover, the impact of the BNI-trait in wheat cultivars was also noticeable for nitrate fertilization, with improved N absorption, and therefore, reducing soil nitrate content.

show abstract

Nitrate reductase activity in nodules of pea inoculated with hydrogenase positive and hydrogenase negative strains of Rhizobium leguminosarum

Cited by 11 publications

References 31 publications

Nitrate metabolism in roots and nodules of Vicia faba in response to exogenous nitrate

Nitrate metabolism in roots and nodules of Vicia faba in response to exogenous nitrate

Nitrogen fixation and nitrate reduction in the root nodules of legumes

Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties

Contact Info

Product

Resources

About