NIR fluorescent ytterbium compound for<i> in vivo</i> fluorescence molecular imaging

Aita, Kazuki; Temma, Takashi; Kuge, Yuji; Seki, Koh‐ichi; Saji, Hideo

doi:10.1002/bio.1136

Cited by 9 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 Developing fluorophores in the NIR window also helps to minimize the optical background that arises from fluorescence from native biological tissue. 11–17 This autofluorescence increases background signal which reduces the all-important signal-to-background ratio (SBR) and makes delineating meaningful signal more difficult.…”

Section: Introductionmentioning

confidence: 99%

“…Optical imaging using near-infrared (NIR) light, however, is harmless as human tissues do not absorb these wavelengths of the electromagnetic spectrum . Developing fluorophores in the NIR window also helps to minimize the optical background that arises from fluorescence from native biological tissue. − This autofluorescence increases background signal which reduces the all-important signal-to-background ratio (SBR) and makes delineating meaningful signal more difficult.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Correlating Molecular Character of NIR Imaging Agents with Tissue-Specific Uptake

et al. 2015

View full text Add to dashboard Cite

Near-infrared (NIR) fluorescent contrast agents are emerging in optical imaging as sensitive, cost-effective, and nonharmful alternatives to current agents that emit harmful ionizing radiation. Developing spectrally distinct NIR fluorophores to visualize sensitive vital tissues to selectively avoid them during surgical resection of diseased tissue is of great significance. Herein, we report the synthetic variation of pentamethine cyanine fluorophores with modifications of physicochemical properties toward prompting tissue-specific uptake into sensitive tissues (i.e., endocrine glands). Tissue-specific targeting and biodistribution studies revealed localization of contrast agents in the adrenal and pituitary glands, pancreas, and lymph nodes with dependence on molecular characteristics. Incorporation of hydrophobic heterocyclic rings, alkyl groups, and halogens allowed a fine-tuning capability to the hydrophobic character and dipole moment for observing perturbation in biological activity in response to minor structural alterations. These NIR contrast agents have potential for clinical translation for intraoperative imaging in the delineation of delicate glands.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Correlating Molecular Character of NIR Imaging Agents with Tissue-Specific Uptake

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Reaction of the probe with hydrazine removes the acetate moiety, producing the highly fluorescent NIR hemicyanine fluorophore. In vitro experiments showed that a linear correlation existed between the fluorescence response and the concentration of the hydrazine in the range 0–50 μM, with a LOD of 1.9 × 10 −7 M. Furthermore, the probe is capable of imaging hydrazine not only in living cells but also in living mice due to its efficient NIR emission, a critical feature for application in bioimaging …”

Section: Probes Based On Acetyl Moietymentioning

confidence: 99%

Recent progress in the development of fluorescent probes for hydrazine

et al. 2018

View full text Add to dashboard Cite

Hydrazine (N H ) is an important and commonly used chemical reagent for the preparation of textile dyes, pharmaceuticals, pesticides and so on. Despite its widespread industrial applications, hydrazine is highly toxic and exposure to this chemical can cause many symptoms and severe damage to the liver, kidneys, and central nervous system. As a consequence, many efforts have been devoted to the development of fluorescent probes for the selective sensing and/or imaging of N H . Although great efforts have been devoted in this area, the large number of important recent studies have not yet been systematically discussed in a review format so far. In this review, we have summarized the recently reported fluorescent N H probes, which are classified into several categories on the basis of the recognition moieties. Moreover, the sensing mechanism and probes designing strategy are also comprehensively discussed on aspects of the unique chemical characteristics of N H and the structures and spectral properties of fluorophores.

show abstract

“…Fluorescent imaging, one of several molecular imaging techniques, is a very convenient method because of its simple and safe operation, high spatial resolution, and short detection time. Fluorescent labels are widely used for applications in biology [1], biotechnology [2], medicine [3], and in combinatorial chemistry [4] We recently reported new NIR fluorescent dyes with low molecular weight, 4AMF-DOTA(Nd) (MW = 883) [5] and PAN-DOTA(Yb) (MW = 823) [6], which include lanthanide complexes in their structures. They had NIR emission and large Stoke's shifts (880 and 900 nm from 488 nm excitation light for 4AMF-DOTA(Nd), 975 nm from 530 nm excitation light for PAN-DOTA(Yb)).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a New NIR Fluorescent Nd Complex Labeling Agent

et al. 2009

Self Cite

View full text Add to dashboard Cite

Fluorescent analysis has been widely used in biological, chemical and analytical research. A useful fluorescent labeling agent should include NIR emission, a large Stoke's shift, and good labeling ability without interfering with the pharmacological profile of the labeled compound. Thus, we planned to develop an M-AMF-DOTA(Nd) derivative composed of an NIR fluorescent moiety and a maleimide conjugating moiety as a new NIR fluorescent labeling agent which fulfills these requirements. M-AMF-DOTA(Nd) was synthesized from 4-amino-fluorescein and was conjugated with an avidin molecule (Avidin-AMF-DOTA(Nd)) through Lys-side chains by reaction with 2-iminothiolane. The fluorescent features of M-AMF-DOTA(Nd) and Avidin-AMF-DOTA(Nd) were comparatively evaluated. A binding assay of Avidin-AMF-DOTA(Nd) with D-biotin and a tumor cell-uptake study were performed to estimate the effects of conjugation on the biological and physicochemical features of the protein. M-AMF-DOTA(Nd) was obtained in 22% overall yield. M-AMF-DOTA(Nd) had a typical NIR fluorescence from the Nd ion (880 nm and 900 nm from 488 nm excitation). Avidin-AMF-DOTA(Nd) was easily synthesized and also had typical NIR fluorescence from the Nd ion without loss of fluorescent intensity. The binding affinity of Avidin-AMF-DOTA(Nd) to D-biotin was equivalent to naive avidin. Avidin-AMF-DOTA(Nd) was taken up by tumor cells in the same manner as avidin conjugated with fluorescein isothiocyanate, an established, widely used fluorescent avidin. Results from this study indicate that M-AMF-DOTA(Nd) is a potential labeling agent for routine NIR fluorescent analysis.

show abstract

NIR fluorescent ytterbium compound for in vivo fluorescence molecular imaging

Cited by 9 publications

References 19 publications

Correlating Molecular Character of NIR Imaging Agents with Tissue-Specific Uptake

Correlating Molecular Character of NIR Imaging Agents with Tissue-Specific Uptake

Recent progress in the development of fluorescent probes for hydrazine

Synthesis of a New NIR Fluorescent Nd Complex Labeling Agent

Contact Info

Product

Resources

About