Nippostrongylus brasiliensis: Phospholipase in nonsensitized and sensitized rats after challenge

Ottolenghi, Athos; Kocan, A. A.; Weatherly, N. F.; Larsh, John E.

doi:10.1016/0014-4894(75)90042-9

Cited by 29 publications

(17 citation statements)

References 13 publications

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“…Since in vivo administered lysophospholipids can be metabolized by lysophospholipases [15,20] to yield biologically inert glyceryl phosphatidyl derivatives, the immunopotentiating effect of lysophospholipids is rather shortlived. Consequently, alkyl analogues of lysophospholipids which contain an ether linkage in place of an ester linkage [17] can also be studied.…”

Section: Effects Of S N-2-methylated Ether Analogues Of Alkyl-lysophomentioning

confidence: 99%

Activation of macrophages by ether analogues of lysophospholipids

Yamamoto

Ngwenya

Sery

et al. 1987

Cancer Immunol Immunother

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Inflammation processes cause activation of phospholipase A in plasma membranes resulting in the production of various lysophospholipids. Treatment of mice with L-alpha-lysophosphatidyl-DL-glycerol (lyso-Pg) resulted in an enhanced ingestion activity of peritoneal macrophages as did other lysophospholipids. However, lyso-Pg is rather toxic as indicated by a rapid decrease in macrophage activity 3 days after treatment while macrophage activity of lysophosphatidylcholine-treated mice continued to increase at least up to the 6th day after treatment. Alkyl-lysophospholipid derivatives, racemic 1-0-octadecyl-2-methylglycero-3-phosphocholine and -phosphoethanolamine stimulated mouse macrophages for Fc-mediated ingestion. Decomposed products of alkyl-lysophospholipids, alkyl-glycerols, were also found to be excellent activators of macrophages not only for ingestion of IgG-coated target cells but also antibody-mediated tumoricidal activity. Macrophages from mice treated with alkylglycerols developed superoxide generating capacity. Furthermore, alkyl-glycerols were found to be tumoricidal by direct contact with retinoblastoma cells. Therefore, the advantage of the potential application of alkylglycerols as chemotherapeutic agents is that they have dual beneficial effects: potentiation of macrophage activity and cytotoxicity to malignant cells.

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Section: Effects Of S N-2-methylated Ether Analogues Of Alkyl-lysophomentioning

confidence: 99%

Activation of macrophages by ether analogues of lysophospholipids

Yamamoto

Ngwenya

Sery

et al. 1987

Cancer Immunol Immunother

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“…Enzymatic activity was estimated as described in detail in previous reports using lysolecithin as the substrate (Ottolenghi. 1970;Ottolenghi & Barnett, 1974;Ottolenghi et al, 1975). Briefly.…”

Section: Phospholipase B Testmentioning

confidence: 99%

“…colon) remain somewhat below the control average even when blood eosinophilia is maximal at 16 times normal. This cannot be ascribed to simple 'saturation' of the sites in question because the intestinal phospholipase pools can expand to 10 times the normal value when rats are infected with Nyppostrongylus braziliensis or T. spiralis (Ottolenghi et al. 1975: Larsh et al.…”

Section: (11) Phospholipase B and Eosinophils After Induction Of Enhamentioning

confidence: 99%

Eosinophilic Leucocytes and Phospholipase B of Rat Tissues

Ottolenghi¹

1986

Cell Proliferation

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The correlation between the eosinophilic leucocyte population and the phospholipase B activity of rat tissues has been tested with isolated cell preparations from intestine, lung, blood, bone marrow and spleen containing eosinophils in varying proportions and with pure eosinophil fractions separated by centrifugation on discontinuous metrizoate and metrizamide gradients. A uniform value of activity per cell was found in all these tissues extending previous histochemical and biochemical evidence that the eosinophil is the carrier cell for the phospholipase B to all major sites of distribution. The enzyme marker approach has been used for estimating the normal eosinophil population of rat organs and show the distribution pattern of the eosinophils in peripheral organs in the wake of increased production and release from the marrow.

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“…Eosinophils or basophils are not always found in tissue with high levels of LPL activity, which suggests that other cells are sources of LPL activity (1 8). The heterogeneous group of pathogens that result in tissue LPL activity proposes that LPL-containing cells are stimulated by a wide variety of antigens, and possibly not by an infectious process (12)(13)(14)(15)(16). The present study was designed to determine whether LPL activity is also associated with neutrophils and whether antigen not found in infectious disease processes will increase LPL activity.…”

mentioning

confidence: 94%

Stimulation of Leukocyte Lysophospholipase Activity by Noninfectious Agents

Hall

Laubach

1991

Experimental Biology and Medicine

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Mouse peritoneal leukocyte lysophospholipase (LPL) activity was studied to determine whether or not noninfectious agents cause increased enzyme activity and whether neutrophils have LPL activity. In the first study, mice infected with Ascaris suum, a known inducer of LPL activity, were given intraperitoneal injections of proteose peptone, thioglycolate, bovine albumin, paraffin, glycogen, or A. suum whole worm extract (WWE). Cell populations collected from mice injected with A. suum WWE, proteose peptone, thioglycolate, or bovine albumin contained increased numbers of neutrophils and eosinophils. These cell populations had increased LPL activity when treated, in vitro, with either A. suum WWE, zymosan-activated complement, or with the agent they were induced with. However, the LPL activity of the different cell populations did not respond to all treatments in the same way. In a second study, A. suum-infected or noninfected mice were given intraperitoneal injections of paraffin, thioglycolate, glycogen, or A. suum WWE. Enriched cell populations containing either lymphocytes or macrophages, from infected or noninfected mice, did not have increased LPL activity following in vitro stimulation with A. suum WWE, zymosan-activated complement, or with the agent they were induced with. Enriched neutrophil populations from infected or noninfected mice had increased LPL activity following in vitro treatment with A. suum WWE or zymosan-activated complement. Results demonstrate that the LPL activity of peritoneal leukocytes can be induced by noninfectious agents and that neutrophils have increased LPL activity following in vitro stimulation.

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Nippostrongylus brasiliensis: Phospholipase in nonsensitized and sensitized rats after challenge

Cited by 29 publications

References 13 publications

Activation of macrophages by ether analogues of lysophospholipids

Activation of macrophages by ether analogues of lysophospholipids

Eosinophilic Leucocytes and Phospholipase B of Rat Tissues

Stimulation of Leukocyte Lysophospholipase Activity by Noninfectious Agents

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