Nile tilapia Toll-like receptor 7 subfamily: Intracellular TLRs that recruit MyD88 as an adaptor and activate the NF-κB pathway in the immune response

Gao, Fengying; Liu, Jie; Lu, Maixin; Liu, Zhigang; Wang, Miao; Ke, Xiaoli; Yi, Ming; Cao, Jianhua

doi:10.1016/j.dci.2021.104173

Cited by 14 publications

(10 citation statements)

References 57 publications

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“…and non-specific antibodies (Gao et al, 2021;Majima-Horiuchi et al, 2018). In this study, both of them were used to stimulate the lymphocytes of pufferfish, causing the up-regulation of Pf-MHC IIα and Pf-MHC IIβ expression (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the expression of Pf-MHC IIα and Pf-MHC IIβ was detected after challenge in vitro and in vivo.Lipopolysaccharide (LPS) is a component of the outer wall of the cell wall of Gram-negative bacterium. It is an endotoxin that can cause an immune response through the toll-like receptor family(Gao et al, 2021;Gnauck et al, 2016). Polyinosinic: polycytidylic acid (Poly I:C) is an analogue of double-stranded RNA that can active lymphocytes and stimulate the body to produce IL-2, IFN F I G U R E 7 Expression patterns of Pf-MHC II (a, b: Pf-MHC IIα, c, d: Pf-MHC IIβ) in pufferfish kidney leukocytes after LPS (a, c) or Poly I:C (b, d) challenge.…”

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confidence: 99%

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Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Qian

Wang

et al. 2023

Journal of Fish Diseases

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Classical major histocompatibility complex (MHC) class II molecules play an essential role in immune system. In this study, MHC IIα (Pf‐MHC IIα) and MHC IIβ (Pf‐MHC IIβ) homology genes from pufferfish (Takifugu obscurus) were cloned and their functional characterization in response to bacterial challenge was identified. The nucleotide sequences of the open reading frames (ORFs) of pufferfish Pf‐MHC IIα and Pf‐MHC IIβ were 708 bp and 750 bp, encoding 235 aa and 249 aa, respectively. The structure of Pf‐MHC IIα or Pf‐MHC IIβ contained a signal peptide, an α1/β1 domain, an α2/β2 domain, a transmembrane region and a cytoplasmic region. Multiple sequence alignment and phylogenetic analysis showed that Pf‐MHC IIα and Pf‐MHC IIβ molecules had the highest similarity with Fugu rubripes (Takifugu rubripes). Cellular localization analysis indicated that the distribution of Pf‐MHC IIα and Pf‐MHC IIβ was in the lymphocyte membrane and cytoplasm. qRT‐PCR results showed that Pf‐MHC IIα and Pf‐MHC IIβ expressed relatively high in skin, gills and gut. In addition, after stimulation challenge in vitro (lipopolysaccharide, or polyinosinic: polycytidylic acid) and in vivo (A. hydrophila), the mRNA expressions of Pf‐MHC IIα and Pf‐MHC IIβ were significantly up‐regulated in lymphocytes and in tissues of skin, gills, gut and head kidney. Moreover, Pf‐MHC IIα or Pf‐MHC IIβ neutralization reduced the ability of A. hydrophila to induce the expressions of lymphocyte cytokines (TNF‐α, IL‐1β and IL‐10). Overall, it is speculated that Pf‐MHC IIα and Pf‐MHC IIβ may play an important role in the host response against A. hydrophila in pufferfish.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Qian

Wang

et al. 2023

Journal of Fish Diseases

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“…Pull‐down assays were performed using previously described procedures (Gao et al ., 2021a; Zhou et al ., 2022). Pull‐down products were detected by Western blotting as described previously (Gao et al ., 2021a; Gao et al ., 2021b).…”

Section: Methodsmentioning

confidence: 99%

“…Interacting proteins were identified by Western blotting with anti‐GST (1:6000) and anti‐Myc antibodies (TRANS, catalogue no: HT101‐01) (1:6000). Other procedures were performed as described previously (Gao et al ., 2021a; Gao et al ., 2021b).…”

Section: Methodsmentioning

confidence: 99%

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Nile tilapia TRIM39 recruits I3K413 and I3KL45 as adaptors and is involved in the NF‐κB pathway

Gao

Zhou

et al. 2022

Journal of Fish Biology

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Tripartite motif (TRIM) proteins play a regulatory function in cancer, cell apoptosis and innate immunity. To understand the role of TRIM39 in Nile tilapia (Oreochromis niloticus), TRIM39 cDNA was isolated. The total length of TRIM39 cDNA was 5025 bp. The deduced OnTRIM39 protein contains 549 amino acids and has conserved domains of the TRIM family, which are the RING, B‐box, coiled‐coil and PRY‐SPRY domains. OnTRIM39 mRNA was widely expressed in various tissues. After challenge with Streptococcus agalactiae and stimulation with polyinosinic polycytidylic acid [poly (I:C)] and lipopolysaccharides (LPS), the amount of OnTRIM39 transcript was changed in various tested tissues. OnTRIM39 overexpression increased NF‐κB activity. OnTRIM39 was present in the cytoplasm. Mass spectrometry of proteins pulled down with recombinant OnTRIM39 showed that 250 proteins potentially interact with OnTRIM39. The authors selected I3K4I3 from the 250 candidate proteins to verify its interaction with TRIM39. They also selected I3KL45, a member of the same 14‐3‐3 protein family, to verify its interaction with TRIM39. The results of pull‐down assays showed that OnTRIM39 interacted with both I3K413 and I3KL45. These results contribute to further study of the innate immune mechanism of tilapia.

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The fish immune armaments in response to pathogen invasion—a tour inside the macrophages

Banerjee

2023

Bacterial Survival in the Hostile Environment

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Nile tilapia Toll-like receptor 7 subfamily: Intracellular TLRs that recruit MyD88 as an adaptor and activate the NF-κB pathway in the immune response

Cited by 14 publications

References 57 publications

Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Nile tilapia TRIM39 recruits I3K413 and I3KL45 as adaptors and is involved in the NF‐κB pathway

The fish immune armaments in response to pathogen invasion—a tour inside the macrophages

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