Nigella sativa as an anti-inflammatory agent in asthma

Abstract

ObjectiveNigella sativa (N. sativa) has several pharmacological actions which include antioxidant, antidiabetic, anticancer, antitussive, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, and bronchodilator. The purpose of this study is to measure the effectivity of N. sativa ethanol extract as anti-inflammation on peritoneal Wistar rat mast cells. The laboratory experiment was used to investigate the effectivity of N. sativa as an anti-inflammatory on mast cells. Six groups of mast cells were stimulated by C 48/80 to release histamine. Group 1 were without N. sativa, while group 2, 3, 4, 5, and 6 were given N. sativa with concentrations of 0.1 mg/ml, 0.2 mg/ml, 0.3 mg/ml, 0.4 mg/ml and 0.5 mg/ml, respectively. Histamine concentration was measured by high-performance liquid chromatography-fluorometry.ResultThe study showed that N. sativa ethanol extract effectively inhibit histamine release from peritoneal Wistar rat mast cells proportionally to its concentration. N. sativa is effective as an anti-inflammation on mast cells by inhibition of histamine release and has no toxic effect on mast cell. N. sativa could be considered as a potential therapy for asthma therapy and prevention.

Anti-Inflammatory, Anti-Arthritic and Anti-Nociceptive Activities of Nigella sativa Oil in a Rat Model of Arthritis

“…One of them is thymoquinone, which is the main bioactive component of the black seeds of Nigella sativa, a plant native to Asian and Arabic countries belonging to the Ranunculaceae family. Many pharmacological actions such as antioxidant, antimicrobial, anti-inflammatory, immunomodulator, analgesic, anti-arthritic, anti-diabetic, anti-asthmatic and anti-cancer have been attributed to thymoquinone and, at least in part, to the other compounds present in Nigella sativa [8,9]. In a previous in vitro study, we demonstrated the antioxidant and anti-inflammatory properties of a crude oil extracted from the seeds of Nigella sativa produced in the Marche region (Italy) [10].…”

In vitro Evaluation of the Anti-inflammatory Effects of Thymoquinone in Osteoarthritis and in silico Analysis of Inter-Related Pathways in Age-Related Degenerative Diseases

“…TQ was reported to downregulate pro-inflammatory genes such as IL-1 , TNF- α, and toll-like receptors ( TLR2 , TLR4 ) and attenuate rheumatoid arthritis via the NFkB pathway ( Arjumand et al, 2019 ). Earlier in vitro and in vivo animal studies have reported that TQ reduces pro-inflammatory cytokines such as TNF- α, COX-2 , and IL-6 in the brain, Schwann cell culture and peritoneal mast cells ( Chen et al, 2016 ; Ikhsan et al, 2018 ). TQ is also reported to offer neuroprotection by reducing the pro-inflammatory cytokines in IFN- γ activated microglial cells via NF-kB dependent signaling ( Cobourne-Duval et al, 2018 ).…”

Therapeutic effects of black seed oil supplementation on chronic obstructive pulmonary disease patients: A randomized controlled double blind clinical trial

“…Extensive clinical and animal researches that have been executed lately have shown that extracts of Nigella sativa (black seed or black cumin) (Family Ranunculaceae), have numerous medicinal and therapeutic properties which may include spasmolytic, a bronchodilator, immunomodulator, antidiabetic, antihistaminic, antimicrobial, anti-inflammatory, and antioxidant effects [ 12 , 13 , 14 , 15 ]. Clinical studies evaluating the impact of Nigella sativa on chronic bronchial asthma [ 16 , 17 , 18 ] showed a marked amelioration in clinical signs and lung function tests.…”