2018
DOI: 10.1111/wrr.12633
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Nifurpirinol: A more potent and reliable substrate compared to metronidazole for nitroreductase‐mediated cell ablations

Abstract: The zebrafish is a popular animal model with well-known regenerative capabilities. To study regeneration in this fish, the nitroreductase/metronidazole-mediated system is widely used for targeted ablation of various cell types. Nevertheless, we highlight here some variability in ablation efficiencies with the metronidazole prodrug that led us to search for a more efficient and reliable compound. Herein, we present nifurpirinol, another nitroaromatic antibiotic, as a more potent prodrug compared to metronidazol… Show more

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Cited by 40 publications
(54 citation statements)
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“…Of note, when higher dose assays were attempted, we observed near complete lethality with 24 h exposure at ≥5 μM NFP. This contrasts with a prior study reporting that lethality initiated at 15 μM NFP for 24 h treatments 32 .…”
Section: Ntr 20 Does Not Improve Nifurpirinol Ablation Efficacycontrasting
confidence: 99%
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“…Of note, when higher dose assays were attempted, we observed near complete lethality with 24 h exposure at ≥5 μM NFP. This contrasts with a prior study reporting that lethality initiated at 15 μM NFP for 24 h treatments 32 .…”
Section: Ntr 20 Does Not Improve Nifurpirinol Ablation Efficacycontrasting
confidence: 99%
“…Contrary to the ~100-fold improvement observed with MTZ, no appreciable enhancement of cell ablation efficacy was observed with nifurpirinol ( Supplementary Fig. S5); cell loss being evident only at 2.5 μM NFP, akin to results with NTR 1.1 32 . This suggests that the enhanced prodrug activity of NTR 2.0 is specific to 5-nitroimidazole prodrugs (e.g.…”
Section: Ntr 20 Does Not Improve Nifurpirinol Ablation Efficacymentioning
confidence: 77%
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