Nicotine-free vapor inhalation produces behavioral disruptions and anxiety-like behaviors in mice: Effects of puff duration, session length, sex, and flavor

Garrett, Patrick I.; Honeycutt, Sarah C.; Marston, Clarissa; Allen, Nicole E.; Barraza, Allyson G.; Dewey, Megan; Turner, Breeann; Peterson, Ashley M.; Hillhouse, Todd M.

doi:10.1016/j.pbb.2021.173207

Cited by 11 publications

(7 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the grounds of these observations, it is evident that this approach is based on the worst toxicological conditions, with the highest risks for users. A similar approach can be found in many articles using these worst risk exposure conditions in studying the effects of EC emissions on cell cultures 4 – 6 and on laboratory animals 7 – 9 .…”

Section: Introductionmentioning

confidence: 98%

Physical and chemical characterizations of a reference e-cigarette used in animal testing

Soulet,

Constans,

Quinty

2023

Sci Rep

View full text Add to dashboard Cite

A minimal necessary condition for preclinical studies to contribute to risk assessments of e-cigarettes (ECs) is the ability to expose laboratory animals to an appropriate dosage of aerosols. In this study, we examined the fulfilment of this essential consistency condition for the ECX-Joyetech E-Vic Mini (ECX), a piece of computerized exposure equipment manufactured by SCIREQ, which has been employed by numerous in vivo testing. We began by calibrating the customary Evic VTC mini device mod and the 4 coils available, reproducing in the laboratory the operation of the ECX in the power-control and temperature-control modes, using puffing parameters recommended by its documentation. We then conducted the following tests for each coil: (1) verifying whether the generated aerosols satisfy an optimal operational regime, free from overheating, as determined by a linear relation between the mass of vaporized e-liquid vs. supplied power and (2) obtaining the mean yields of aldehydes for each of the tested power settings and coils. The results of these tests show that, under the main conditions used in in vivo testing, the ECX equipment fails to comply with these consistency requirements, especially for coils with low subohm resistance, a shortcoming that can be corrected by applying much larger airflows for these coils. Therefore, the outcomes of preclinical studies using the ECX equipment should be examined with great scepticism and subjected to further testing.

show abstract

Section: Introductionmentioning

confidence: 98%

Physical and chemical characterizations of a reference e-cigarette used in animal testing

Soulet,

Constans,

Quinty

2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…MiceVAPORDot has the potential to be a powerful tool to fill the blank of assessing the behavior during the aerosol exposure procedure. Although previous studies have demonstrated that e-cigarette nicotine exposure could influence the behaviors of mice (Garrett et al, 2021; Grant et al, 2016; Ponzoni et al, 2020) no work have characterized behavioral alterations during smoke exposure. The key obstacle could be animal tracking under the occlusion of e-cigarette aerosols.…”

Section: Discussionmentioning

confidence: 99%

MiceVAPORDot: A novel automated approach for high-throughput behavioral characterization during E-cigarette exposure in mice

Han,

Xu,

et al. 2023

Preprint

View full text Add to dashboard Cite

The prosperity of electronic nicotine delivery systems (ENDS) or e-cigarette use has been regarded to lead an increasing risk of nicotine addiction, especially among youth. Understanding and evaluating the behaviors induced by ENDS are fundamental to the study of neuropsychiatric effects of e-cigarettes. However, little is known regarding the behavioral features during e-cigarette exposure in mice. Current behavioral assessments for nicotine addiction are based on nicotine withdrawal-induced anxiety which can be only performed after ENDS exposures. Here we developed MiceVAPORDot, a novel high-throughput tool for automatedin situbehavioral characterization during e-cigarette exposure. The integration of a deep learning-based animal pose tracking method by MiceVAPORDot allows precise characterization on behavioral phenotypes of e-vapor exposed mice, which were unable revealed by traditional evaluation methodology such as conditioned place preference and elevated plus maze tests. The behavioral fingerprints recognized by MiceVAPORDot can be used for high-throughput screening on incentive nature of e-cigarette flavors as well as medications for smoking cessation.

show abstract

“…However, the development of ENDS allows the study of nicotine effects by using the same route of administration of clinical populations. While some researchers have begun to employ ENDS-based vapor delivery systems to study the neurobiological effects of chronic nicotine exposure 15 – 17 or other substances 18 , most studies to date have investigated a limited number of dosing regimens or behavioral outputs.…”

Section: Introductionmentioning

confidence: 99%

Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice

Murdaugh,

Miliano,

Chen

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Nicotine use is a leading cause of preventable deaths worldwide, and most of those who attempt to quit will relapse. While electronic cigarettes and other electronic nicotine delivery systems (ENDS) were presented as a safer alternative to traditional cigarettes and promoted as devices to help traditional tobacco smokers reduce or quit smoking, they have instead contributed to increasing nicotine use among youths. Despite this, ENDS also represent a useful tool to create novel preclinical animal models of nicotine exposure that more accurately represent human nicotine use. In this study, we validated a chronic, intermittent, ENDS-based passive vapor exposure model in mice, and then measured changes in multiple behaviors related to nicotine abstinence. First, we performed a behavioral dose curve to investigate the effects of different nicotine inter-vape intervals on various measures including body weight, locomotor activity, and pain hypersensitivity. Next, we performed a pharmacokinetic study to measure plasma levels of nicotine and cotinine following chronic exposure for each inter-vape interval. Finally, we utilized a behavior test battery at a single dosing regimen that produces blood levels equivalent to human smokers in order to characterize the effects of chronic nicotine, vehicle, or passive airflow and identified nicotine-induced impairments in cognitive behavior.

show abstract

Nicotine-free vapor inhalation produces behavioral disruptions and anxiety-like behaviors in mice: Effects of puff duration, session length, sex, and flavor

Cited by 11 publications

References 54 publications

Physical and chemical characterizations of a reference e-cigarette used in animal testing

Physical and chemical characterizations of a reference e-cigarette used in animal testing

MiceVAPORDot: A novel automated approach for high-throughput behavioral characterization during E-cigarette exposure in mice

Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice

Contact Info

Product

Resources

About