Nicotinamide and ascorbic acid nanoparticles against the hepatic insult induced in rats by high fat high fructose diet: A comparative study

Abd-Allah, Hend; Nasr, Maha; Ahmed-Farid, Omar A.; Ibrahim, Bassant M. M.; Bakeer, Rofanda M.; Ahmed, Rania F.

doi:10.1016/j.lfs.2020.118540

Cited by 15 publications

(14 citation statements)

References 59 publications

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“…Nicotinamide and ascorbic acid chitosan nanoparticles were prepared using the ionotropic gelation method as previously described elsewhere. 34 Four nanoparticle formulations were prepared, of low concentration (2.5 mg/mL) of either nicotinamide (NL) or ascorbic acid (AL) and another two of high concentration (5 mg/mL) of either drugs (NH and AH), respectively. The formulations were characterized for particle size, for surface charge using a Zetasizer device (Nano ZS 3600, Malvern, U.K.), and for drugs' entrapment after separation of the unentrapped drugs by centrifugation (Hermle Labortechnik GmbH, Germany) and spectrophotometric analysis at 262 and 265 nm for nicotinamide and ascorbic acid, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The formulations were characterized for particle size, for surface charge using a Zetasizer device (Nano ZS 3600, Malvern, U.K.), and for drugs' entrapment after separation of the unentrapped drugs by centrifugation (Hermle Labortechnik GmbH, Germany) and spectrophotometric analysis at 262 and 265 nm for nicotinamide and ascorbic acid, respectively. 34 2.3. In Vivo Experiment.…”

Section: Methodsmentioning

confidence: 99%

“…Nicotinamide and ascorbic acid chitosan nanoparticles were prepared using the ionotropic gelation method as previously described elsewhere . Four nanoparticle formulations were prepared, of low concentration (2.5 mg/mL) of either nicotinamide (NL) or ascorbic acid (AL) and another two of high concentration (5 mg/mL) of either drugs (NH and AH), respectively.…”

Section: Experimental Sectionmentioning

confidence: 99%

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Biological and Pharmacological Characterization of Ascorbic Acid and Nicotinamide Chitosan Nanoparticles against Insulin-Resistance-Induced Cognitive Defects: A Comparative Study

et al. 2021

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High consumption of industrialized food with high fat content is generally associated with insulin resistance, which in turn causes memory impairment and cognitive decline. Nicotinamide and ascorbic acid are among the promising neuroprotective molecules; however, an appreciable therapeutic activity necessitates the administration of a large dose of either. Therefore, the study aimed to assess if loading them in chitosan nanoparticles in doses 5–10 times lower than the unencapsulated forms would achieve comparable therapeutic results. Animals were fed a high-fat-high-fructose (HFHF) diet for 75 days. The vitamins in their conventional form (100 mg/kg) and the nanoparticles under investigation (10 and 20 mg/kg) were given orally concomitantly with the diet in the last 15 days. The intake of HFHF diet for 75 days led to an insulin-resistant state, with memory impairment, which was verified behaviorally through the object recognition test. This was accompanied by significant reduction in brain insulin-like growth factor 1 (IGF-1), increased acetylcholine esterase activity, increase in the serotonin and dopamine turnover ratio, and increase in oxidative stress and 8-OHdG, indicating cellular DNA fragmentation. Cellular energy was also decreased, and immunohistochemical examination verified the high immunoreactivity in both the cortex and hippocampus of the brain. The administration of nanoparticulated nicotinamide or ascorbic acid with a 10 times lesser dose than the unencapsulated forms managed to reverse all aforementioned harmful effects, with an even lesser immunoreactivity score than the unencapsulated form. Therefore, it can be concluded that nicotinamide or ascorbic acid chitosan nanoparticles can be recommended as daily supplements for neuroprotection in patients suffering from insulin resistance after conduction of clinical investigations.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Experimental Sectionmentioning

confidence: 99%

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Biological and Pharmacological Characterization of Ascorbic Acid and Nicotinamide Chitosan Nanoparticles against Insulin-Resistance-Induced Cognitive Defects: A Comparative Study

et al. 2021

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“…Moreover, chitosan has gained attention as biomedical material [13,14] due to its multifaceted activities, such as anti-inflammatory [15], antibacterial [16], antitumoral [17][18][19], immunostimulatory [20], anti-hyperglycemic and anti-hyperlipidemic [21,22], antiulcer [23] and antioxidant [24,25] activities. The literature data highlight that this polymer can be formulated into various dosage forms: solutions, gels, solid mixtures, sponges, prolongedrelease tablets, pastes and nanoparticles, for different applications [26].…”

Section: Introductionmentioning

confidence: 99%

The Use of Chitosan-Coated Nanovesicles in Repairing Alcohol-Induced Damage of Liver Cells in Mice

et al. 2022

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Background and Objectives In the past few decades, the studies concerning the natural polysaccharide chitosan have been centered on a new direction: its hepatoprotective action. The aim of our study was to evaluate the influence of previously designed chitosan lipid vesicles on the liver damage induced by alcohol consumption in mice. Materials and Methods The study involved the oral administration of substances in one daily dose as follows: Group 1 (control): water; Group 2 (control alcohol): 5% alcohol in water; Group 3 (CHIT): 0.1 mL/10 g body weight chitosan solution in animals treated with alcohol; Group 4 (CHIT-ves): 0.1 mL/10 g body chitosan vesicles in animals treated with alcohol; Group 5 (AcA): 200 mg/kg body ascorbic acid in animals treated with alcohol. In order to evaluate liver damage after alcohol consumption, the following hematological parameters were tested: the activity of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase; serum values of urea and creatinine; the phagocytic capacity of polymorphonuclear neutrophilsin peripheral blood;serum opsonic capacity;bactericidal capacity of peritoneal macrophages; and the activity of malondialdehyde, glutathione peroxidase, superoxide dismutase and lactate dehydrogenase. Results and Conclusion The treatment with chitosan vesicles decreased liver enzyme activity and reduced the oxidative stress disturbances in alcoholic mice, thus repairing the hepatic functional and structural damages. These beneficial activities of chitosan vesicles were comparable with ascorbic acid effects in alcoholic mice.

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“…NAFLD can gradually evolve from the simple steatosis or nonalcoholic fatty liver to nonalcoholic steatohepatitis, cirrhosis, and eventually liver cancer . The development and progression of NAFLD are largely related to insulin resistance, oxidative stress, and lipid/glucose metabolism . However, the pathogenesis of NAFLD is very complicated and has not been fully elucidated so far.…”

Section: Introductionmentioning

confidence: 99%

Construction of Glycogen-Based Nanoparticles Loaded with Resveratrol for the Alleviation of High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease

Chen

et al. 2021

Biomacromolecules

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The purpose of this study was to construct a glycogen (Gly)-based nanoparticle (NP) with liver-targeted and redox response to effectively deliver resveratrol (Res) for improving nonalcoholic fatty liver disease (NAFLD). Herein, Gly was modified using α-lipoic acid (α-LA) and lactobionic acid (Lac) to obtain an amphiphilic polymer (Gly−LA−Lac), which was self-assembled in water and then encapsulated in Res to form Res NPs with excellent stability. As expected, the Res NPs exhibited liver-targeted and redox response release behavior. In vitro cell studies demonstrated that the nanocarrier treatment enhanced the cellular uptake of Res and reduced oxidative stress and inflammatory factor levels. Meanwhile, the in vivo tests proved that the nanocarriers effectively reduced hepatic lipid accumulation and oxidative stress levels via regulating the TLR4/NF-κB signal pathway to improve liver damage in NAFLD mice. In conclusion, this study provides a promising strategy through the construction of Gly-based nanocarriers for the encapsulation of Res to effectively alleviate the process of NAFLD.

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Nicotinamide and ascorbic acid nanoparticles against the hepatic insult induced in rats by high fat high fructose diet: A comparative study

Cited by 15 publications

References 59 publications

Biological and Pharmacological Characterization of Ascorbic Acid and Nicotinamide Chitosan Nanoparticles against Insulin-Resistance-Induced Cognitive Defects: A Comparative Study

Biological and Pharmacological Characterization of Ascorbic Acid and Nicotinamide Chitosan Nanoparticles against Insulin-Resistance-Induced Cognitive Defects: A Comparative Study

The Use of Chitosan-Coated Nanovesicles in Repairing Alcohol-Induced Damage of Liver Cells in Mice

Construction of Glycogen-Based Nanoparticles Loaded with Resveratrol for the Alleviation of High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease

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