Nicotinamide abrogates acute lung injury caused by ischaemia/reperfusion

Su, Chain-Fa; Liu, Demeral David; Kao, Shu Huei; Chen, H. I.

doi:10.1183/09031936.00025107

Cited by 36 publications

(36 citation statements)

References 32 publications

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“…Previous studies have reported that niacinamide exerts protective effects on the acute lung injury induced by various challenges including endotoxin, ischemia/reperfusion, and oxidative stress [7,9,20,35-38]. In the present investigation, we found that this cytoprotective agent effectively abrogated the lung injury caused by phorbol myristate acetate in isolated lungs.…”

Section: Discussionsupporting

confidence: 67%

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Niacinamide mitigated the acute lung injury induced by phorbol myristate acetate in isolated rat's lungs

et al. 2012

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BackgroundPhorbol myristate acetate (PMA) is a strong neutrophil activator and has been used to induce acute lung injury (ALI). Niacinamide (NAC) is a compound of B complex. It exerts protective effects on the ALI caused by various challenges. The purpose was to evaluate the protective effects of niacinamide (NAC) on the PMA-induced ALI and associated changes.MethodsThe rat's lungs were isolated in situ and perfused with constant flow. A total of 60 isolated lungs were randomized into 6 groups to received Vehicle (DMSO 100 μg/g), PMA 4 μg/g (lung weight), cotreated with NAC 0, 100, 200 and 400 mg/g (lung weight). There were 10 isolated lungs in each group. We measured the lung weight and parameters related to ALI. The pulmonary arterial pressure and capillary filtration coefficient (Kfc) were determined in isolated lungs. ATP (adenotriphosphate) and PARP [poly(adenosine diphophate-ribose) polymerase] contents in lung tissues were detected. Real-time PCR was employed to display the expression of inducible and endothelial NO synthases (iNOS and eNOS). The neutrophil-derived mediators in lung perfusate were determined.ResultsPMA caused increases in lung weight parameters. This agent produced pulmonary hypertension and increased microvascular permeability. It resulted in decrease in ATP and increase in PARP. The expression of iNOS and eNOS was upregulated following PMA. PMA increased the neutrophil-derived mediators. Pathological examination revealed lung edema and hemorrhage with inflammatory cell infiltration. Immunohistochemical stain disclosed the presence of iNOS-positive cells in macrophages and endothelial cells. These pathophysiological and biochemical changes were diminished by NAC treatment. The NAC effects were dose-dependent.ConclusionsOur results suggest that neutrophil activation and release of neutrophil-derived mediators by PMA cause ALI and associated changes. NO production through the iNOS-producing cells plays a detrimental role in the PMA-induced lung injury. ATP is beneficial, while PARP plays a deteriorative effect on the PMA-induced ALI. NAC exerts protective effects on the inflammatory cascade leading to pulmonary injury. This B complex compound may be applied for clinical usage and therapeutic regimen.

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Section: Discussionsupporting

confidence: 67%

“…NAC was given simultaneously with PMA [20,37,38]. Examinations of biochemical factors were taken before administration of PMA and NAC, and at the end of the experiments.…”

Section: Methodsmentioning

confidence: 99%

Niacinamide mitigated the acute lung injury induced by phorbol myristate acetate in isolated rat's lungs

et al. 2012

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“…As such, supplementation may be useful over a broad range of metabolic disorders. In the clinical setting, niacinamide is used primarily for the treatment of acne and pellagra; however, niacinamide has also been studied in insulin-dependent diabetes, where it may preserve pancreatic ␤ cells (12), and ischemia-reperfusion injury, where it inhibits nitric oxide synthase (13). Niacinamide may mediate a variety of protective mechanisms through downregulation of TNF-␣ (14), increased free radical scavenging (15), and inhibition of poly-ADP-ribose synthetase, an emerging target for cardiovascular disease and cancer (16).…”

Section: Discussionmentioning

confidence: 99%

A Randomized, Double-Blind, Placebo-Controlled Trial of Niacinamide for Reduction of Phosphorus in Hemodialysis Patients

Cheng

Young

Huang

et al. 2008

Clinical Journal of the American Society of Nephrology

104

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Background and objectives: Niacinamide inhibits intestinal sodium/phosphorus transporters and reduces serum phosphorus in open-label studies. A prospective, randomized, double-blind, placebo-controlled crossover trial was performed for assessment of the safety and efficacy of niacinamide.Design, setting, participants, & measurements: Hemodialysis patients with phosphorus levels >5.0 mg/dl were randomly assigned to 8 wk of niacinamide or placebo, titrated from 500 to 1500 mg/d. After a 2-wk washout period, patients switched to 8 wk of the alternative therapy. Vitamin D analogs and calcimimetics were held constant; phosphorus binders were not changed unless safety criteria were met.Results: Thirty-three patients successfully completed the trial. Serum phosphorus fell significantly from 6.26 to 5.47 mg/dl with niacinamide but not with placebo (5.85 to 5.98 mg/dl). A concurrent fall in calcium-phosphorus product was seen with niacinamide, whereas serum calcium, intact parathyroid hormone, uric acid, platelet, triglyceride, LDL, and total cholesterol levels remained stable in both arms. Serum HDL levels rose with niacinamide (50 to 61 mg/dl but not with placebo. Adverse effects were similar between both groups. Among patients who were >80% compliant, results were similar, although the decrease in serum phosphorus with niacinamide was more pronounced (6.45 to 5.28 mg/dl) and the increase in HDL approached significance (49 to 58 mg/dl).Conclusions: In hemodialysis patients, niacinamide effectively reduces serum phosphorus when co-administered with binders and results in a potentially advantageous increase in HDL cholesterol. Further study in larger randomized trials and other chronic kidney disease populations is indicated.

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“…Allografted airways treated with INO-1001 demonstrated attenuated NF-B nuclear translocation and reduced transcription of tumour necrosis factor (TNF)-, indicating proinflammatory effects of PARP-1 activation [60]. Recently, Su et al [61] have demonstrated elevated levels of poly-ADP-ribose in the lungs following I/R. Moreover, nicotinamide reduced the expression of inducible nitric oxydase synthase (iNOS) and pro-inflammatory cytokines, as well as the levels of nitric oxide (NO) and free radicals, while restoring ATP levels.…”

Section: Parp-1 In Lung Disordersmentioning

confidence: 99%

“…Moreover, nicotinamide reduced the expression of inducible nitric oxydase synthase (iNOS) and pro-inflammatory cytokines, as well as the levels of nitric oxide (NO) and free radicals, while restoring ATP levels. These effects were suggested to be due to PARP-1 inhibition [61].…”

Section: Parp-1 In Lung Disordersmentioning

confidence: 99%

NAD+-Consuming Enzymes in the Regulation of Lung Immune Responses

Legutko¹,

Lekeux²,

Bureau³

2009

TOIJ

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Nicotinamide adenine dinucleotide (NAD+) plays a role as a coenzyme in numerous oxidation-reduction reactions and mediates not only energy metabolism and mitochondrial functions, but also calcium homeostasis, aging, and cell death. Moreover, extensive evidence attests to the great importance of the non-redox functions of NAD+ via NAD+-consuming enzymes. Indeed, ADP-ribose transferases, cADP-ribose synthases and sirtuins emerge as NAD+ dependent enzymes with potent regulatory function in many physiological and pathophysiological processes. They have been shown to be involved in several neurological and cardiovascular disorders as well as in cancer and inflammation. In this review we will summarize the current knowledge about function of NAD+-consuming enzymes in the regulation of the immune system with particular emphasis on lung inflammatory disorders.

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Nicotinamide abrogates acute lung injury caused by ischaemia/reperfusion

Cited by 36 publications

References 32 publications

Niacinamide mitigated the acute lung injury induced by phorbol myristate acetate in isolated rat's lungs

Niacinamide mitigated the acute lung injury induced by phorbol myristate acetate in isolated rat's lungs

A Randomized, Double-Blind, Placebo-Controlled Trial of Niacinamide for Reduction of Phosphorus in Hemodialysis Patients

NAD+-Consuming Enzymes in the Regulation of Lung Immune Responses

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